Quantitation of Acetyl Hexapeptide-8 in Cosmetics by Hydrophilic Interaction Liquid Chromatography Coupled to Photo Diode Array Detection
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemical and Reagents
2.2. Stock and Calibration Standard Solutions
2.3. Sample Preparation Procedure
2.3.1. Cosmetic Formulation
2.3.2. Cosmetic Cream
2.4. HILIC-PDA
2.5. Method Validation and Application to the Analysis of Real Samples
3. Results and Discussions
3.1. HILIC-PDA Method Development
Chromatography
3.2. Statistical Analysis of Data
3.2.1. Selectivity
3.2.2. Linearity Data
3.2.3. Accuracy and Precision
3.3. Application to the Analysis of Real Samples
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Concentration Range (μg mL−1) | 20–30 |
Regression Equation | S = 18.08 (±0.64) × 103 C − 20 (±16) × 103 |
Correlation Coefficient, R | 0.998 |
Standard Error of Estimation, Sr | 5072 |
Limit of Detection, LOD (μg mL−1) | 0.5 |
Limit of Quantitation, LOQ (μg mL−1) | 1.5 |
texperimental: a/Sa 1 | 1.24 |
tp, f = 3, p = 0.05 2 | 4.30 |
Concentration Range (% w/w) | 0.004–0.007 |
Regression Equation | S = 1105 (±41) × C + 0.71 (±0.23) |
Correlation Coefficient, r | 0.998 |
Standard Error of Estimation, Sr | 0.098 |
Limit of Detection, LOD (% w/w) | 6.8 × 10−4 |
Limit of Quantitation, LOQ (% w/w) | 0.002 |
texperimental: a/Sa 1 | 3.10 |
tp, f = 3, p = 0.05 2 | 4.30 |
Matrix | Concentration Levels | |||
---|---|---|---|---|
Cosmetic Formulation | ||||
Added concentration (μg mL−1) | 20 | 25 | ||
Overall mean | 19.95 (±0.91) | 24.74 (±0.42) | ||
Intraday CV(%)1 | 4.76 | 1.66 | ||
Total precision CV(%) 1 | 4.37 | 1.74 | ||
Total accuracy Er%2 | 99.8 | 98.9 | ||
Cosmetic cream | ||||
Added concentration (% w/w) | 0.004 | 0.005 | 0.007 | |
Overall mean | 39.7 (±0.82) × 10−4 | 50.62 (±0.39) × 10−4 | 69.71 (±0.79) × 10−4 | |
Intraday, CV(%) 1 | 2.41 | 3.33 | 3.11 | |
Total precision, CV(%) 1 | 2.46 | 3.35 | 3.53 | |
Total accuracy, Relative Recovery (%) 2 | 99.3 | 101.2 | 99.6 |
Cosmetic Product | % Label Claim (±SD) 1 (n = 5) | % CV 2 |
---|---|---|
Cosmetic formulation | ||
Batch No F1 | 0.0511 (±0.0015) | 2.93 |
Batch No F2 | 0.0507 (±0.0011) | 2.16 |
Batch No F3 | 0.0491 (±0.0016) | 3.25 |
Cosmetic cream | ||
Batch No C1 | 0.00491 (±0.00018) | 3.7 |
Batch No C2 | 0.00501 (±0.00013) | 2.6 |
Batch No C3 | 0.00509 (±0.00021) | 4.1 |
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Raikou, V.; Kalogria, E.; Varvaresou, A.; Tsirivas, E.; Panderi, I. Quantitation of Acetyl Hexapeptide-8 in Cosmetics by Hydrophilic Interaction Liquid Chromatography Coupled to Photo Diode Array Detection. Separations 2021, 8, 125. https://doi.org/10.3390/separations8080125
Raikou V, Kalogria E, Varvaresou A, Tsirivas E, Panderi I. Quantitation of Acetyl Hexapeptide-8 in Cosmetics by Hydrophilic Interaction Liquid Chromatography Coupled to Photo Diode Array Detection. Separations. 2021; 8(8):125. https://doi.org/10.3390/separations8080125
Chicago/Turabian StyleRaikou, Vasiliki, Eleni Kalogria, Athanasia Varvaresou, Efthimios Tsirivas, and Irene Panderi. 2021. "Quantitation of Acetyl Hexapeptide-8 in Cosmetics by Hydrophilic Interaction Liquid Chromatography Coupled to Photo Diode Array Detection" Separations 8, no. 8: 125. https://doi.org/10.3390/separations8080125