What You Extract Is What You Get: Different Methods of Protein Extraction from Hemp Seeds
Dipartimento di Scienze e Innovazione Tecnologica-DiSIT, Università del Piemonte Orientale, 13100 Vercelli, Italy
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to the work.
Academic Editor: Ernesto Reverchon
Separations 2021, 8(12), 231; https://doi.org/10.3390/separations8120231
Received: 25 October 2021
/
Revised: 23 November 2021
/
Accepted: 30 November 2021
/
Published: 2 December 2021
(This article belongs to the Special Issue Extraction and Analysis of Plant Active Ingredients)
Cannabis sativa L. seeds are rich in essential polyunsaturated fatty acids and highly digestible proteins, with a good nutritional value. Proteomics studies on hempseed reported so far have mainly been conducted on processed seeds and, to our knowledge, no optimization of protein extraction from hemp seeds has been performed. This study investigates the SDS-PAGE profile of hempseed proteins comparing different methods of extraction, (Osborne sequential extraction, TCA/acetone, MTBE/methanol, direct protein solubilization of defatted hempseed flour), two conditions to keep low temperature during seed grinding (liquid nitrogen or ice) and two solubilization buffers (urea-based or Laemmli buffer). Among the tested conditions, the combination of liquid nitrogen + TCA/acetone + Laemmli buffer was not compatible with SDS-PAGE of proteins. On the other hand, urea-based buffer achieved more reproducible results if combined with all the other conditions. TCA/acetone, MTBE/methanol, and direct protein solubilization of defatted hempseed flour demonstrated a good overview of protein content, but less abundant proteins were poorly represented. The Osborne sequential separation was helpful in diluting abundant proteins thus enhancing the method sensitivity.
View Full-Text
Keywords:
protein extraction; hempseed; seed storage proteins; SDS-PAGE
▼
Show Figures
Figure 1
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
- Supplementary File 1:
ZIP-Document (ZIP, 526 KiB)
MDPI and ACS Style
Givonetti, A.; Cattaneo, C.; Cavaletto, M. What You Extract Is What You Get: Different Methods of Protein Extraction from Hemp Seeds. Separations 2021, 8, 231. https://doi.org/10.3390/separations8120231
AMA Style
Givonetti A, Cattaneo C, Cavaletto M. What You Extract Is What You Get: Different Methods of Protein Extraction from Hemp Seeds. Separations. 2021; 8(12):231. https://doi.org/10.3390/separations8120231
Chicago/Turabian StyleGivonetti, Annalisa, Chiara Cattaneo, and Maria Cavaletto. 2021. "What You Extract Is What You Get: Different Methods of Protein Extraction from Hemp Seeds" Separations 8, no. 12: 231. https://doi.org/10.3390/separations8120231
Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.
