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Article

Separation of the Glycosylated Carotenoid Myxoxanthophyll from Synechocystis Salina by HPCCC and Evaluation of Its Antioxidant, Tyrosinase Inhibitory and Immune-Stimulating Properties

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Laboratory of Algal Biotechnology—Centre ALGATECH, Institute of Microbiology of the Czech Academy of Sciences, Opatovický Mlýn, 379 81 Třeboň, Czech Republic
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Department of Pharmacognosy, Faculty of Pharmacy, Charles University, Akademika Heyrovského 1203, 500 05 Hradec Králové, Czech Republic
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Institute of Clinical Immunology and Allergology, Faculty of Medicine and University Hospital, Charles University, Sokolská 581, 500 05 Hradec Králové, Czech Republic
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Contipro a.s., Dolní Dobrouč 401, 561 02 Dolní Dobrouč, Czech Republic
*
Author to whom correspondence should be addressed.
Separations 2020, 7(4), 73; https://doi.org/10.3390/separations7040073
Received: 23 November 2020 / Revised: 7 December 2020 / Accepted: 10 December 2020 / Published: 15 December 2020
(This article belongs to the Special Issue Applications of Liquid–Liquid Chromatography)
Global demand for natural pigments has increased in the past few years. Myxoxanthophyll, a glycosylated monocyclic carotenoid, is a pigment that occurs naturally in cyanobacteria but no scalable isolation process has been developed to obtain it from its natural source to date. In this study, myxoxanthophyll was isolated from unicellular cyanobacterium Synechocystis salina (S. salina) using high-performance countercurrent chromatography (HPCCC), where the lower phase of the biphasic solvent system composed of n-heptane–ethanol–water (2:4:4, v/v/v) was used as a mobile phase, whereas its upper phase was the stationary phase. For the HPCCC isolation, a multi-injection method was developed, and four consecutive sample injections (70 mg each) were performed, obtaining, in total, 20 mg of myxoxanthophyll, which was finally purified with high-performance liquid chromatography (HPLC). Overall, a final myxoxanthophyll yield of 15 mg (98% purity) was obtained. The target pigment showed a weak antioxidant and tyrosinase inhibitory effect, and exhibited immune-stimulating properties by activating human granulocytes. The results presented here form a basis for the large-scale production of myxoxanthophyll, and show the potential benefits of this pigment for human health. View Full-Text
Keywords: myxoxanthophyll; cyanobacteria; Synechocystis; high-performance countercurrent chromatography (HPCCC); countercurrent chromatography (CCC); centrifugal partition chromatography (CPC) myxoxanthophyll; cyanobacteria; Synechocystis; high-performance countercurrent chromatography (HPCCC); countercurrent chromatography (CCC); centrifugal partition chromatography (CPC)
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MDPI and ACS Style

Nováková, M.; Fábryová, T.; Vokurková, D.; Dolečková, I.; Kopecký, J.; Hrouzek, P.; Tůmová, L.; Cheel, J. Separation of the Glycosylated Carotenoid Myxoxanthophyll from Synechocystis Salina by HPCCC and Evaluation of Its Antioxidant, Tyrosinase Inhibitory and Immune-Stimulating Properties. Separations 2020, 7, 73. https://doi.org/10.3390/separations7040073

AMA Style

Nováková M, Fábryová T, Vokurková D, Dolečková I, Kopecký J, Hrouzek P, Tůmová L, Cheel J. Separation of the Glycosylated Carotenoid Myxoxanthophyll from Synechocystis Salina by HPCCC and Evaluation of Its Antioxidant, Tyrosinase Inhibitory and Immune-Stimulating Properties. Separations. 2020; 7(4):73. https://doi.org/10.3390/separations7040073

Chicago/Turabian Style

Nováková, Michaela, Tereza Fábryová, Doris Vokurková, Iva Dolečková, Jiří Kopecký, Pavel Hrouzek, Lenka Tůmová, and José Cheel. 2020. "Separation of the Glycosylated Carotenoid Myxoxanthophyll from Synechocystis Salina by HPCCC and Evaluation of Its Antioxidant, Tyrosinase Inhibitory and Immune-Stimulating Properties" Separations 7, no. 4: 73. https://doi.org/10.3390/separations7040073

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