Next Article in Journal
Exploring New Extractive Phases for In-Tube Solid Phase Microextraction Coupled to Miniaturized Liquid Chromatography
Next Article in Special Issue
Total and Free Fatty Acids Analysis in Milk and Dairy Fat
Previous Article in Journal
Extraction and Isolation of Kaempferol Glycosides from the Leaves and Twigs of Lindera neesiana
Article Menu
Issue 1 (March) cover image

Export Article

Open AccessArticle

Biogenic Amines in Traditional Fiore Sardo PDO Sheep Cheese: Assessment, Validation and Application of an RP-HPLC-DAD-UV Method

Dipartimento di Chimica e Farmacia, Università degli Studi di Sassari, via Vienna 2, 07100 Sassari, Italy
Agris Sardegna, Loc. Bonassai Km.18,600, 07040 Olmedo, Italy
Author to whom correspondence should be addressed.
Separations 2019, 6(1), 11;
Received: 31 December 2018 / Revised: 5 February 2019 / Accepted: 12 February 2019 / Published: 19 February 2019
(This article belongs to the Special Issue Separation Techniques for Dairy Analysis)
PDF [1497 KB, uploaded 27 February 2019]


This contribution aimed to measure for the first time the amount of biogenic amines (BAs) in one of the most ancient and traditional sheep cheese produced in Sardinia, Italy: the Protected Designation of Origin (PDO) Fiore Sardo. To achieve this, an original RP-HPLC-DAD-UV method has been developed that was completely validated in terms of LoD, LoQ, linearity, precision and trueness, and tested on 36 real Fiore Sardo PDO cheese samples produced by four different cheesemakers and marketed by four stores. The average total concentration of the eight BAs (i.e., tyramine, tryptamine, histidine, putrescine, cadaverine, 2-phenylethylamine, spermine and spermidine) measured in Fiore Sardo cheese was 700 mg/kg, with a range between 170 mg/kg and 1100 mg/kg. A great variability in the total amount of BAs has been evidenced among the Fiore Sardo marketed in the four stores as well as for the cheeses purchased in different times in the same store. Tyramine (350 mg/kg), putrescine (150 mg/kg), histamine (80 mg/kg) and cadaverine (30 mg/kg) are the most abundant BAs found in this matrix. Among the many factors concurring, the dominant microflora of Fiore Sardo PDO is likely the principal cause of the qualitative and quantitative distribution of BAs in this matrix. Finally, the total amount of BAs found in Fiore Sardo PDO is not able to cause any health alert situation for consumers. View Full-Text
Keywords: Fiore Sardo PDO; biogenic amines; sheep’s cheese; RP-HPLC Fiore Sardo PDO; biogenic amines; sheep’s cheese; RP-HPLC

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Share & Cite This Article

MDPI and ACS Style

Zazzu, C.; Addis, M.; Caredda, M.; Scintu, M.F.; Piredda, G.; Sanna, G. Biogenic Amines in Traditional Fiore Sardo PDO Sheep Cheese: Assessment, Validation and Application of an RP-HPLC-DAD-UV Method. Separations 2019, 6, 11.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Metrics

Article Access Statistics



[Return to top]
Separations EISSN 2297-8739 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top