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Separations 2018, 5(2), 21; https://doi.org/10.3390/separations5020021

Supercritical CO2 Extracts and Volatile Oil of Basil (Ocimum basilicum L.) Comparison with Conventional Methods

1
Chemical Engineering and Biotechnology Research Center, Instituto Superior de Engenharia de Lisboa, IPL, 1959-007 Lisboa, Portugal
2
Centro de Química Estrutural, Instituto Superior Técnico, Universidade de Lisboa, 1049-001 Lisboa, Portugal
3
CITAB-Centre for the Research and Technology of Agro-Environmental and Biological Sciences, University of Trás-os-Montes and Alto Douro, 5000-801 Vila Real, Portugal
4
CBiOS, Research Center for Biosciences & Health Technologies, ULHT, 1749-024 Lisboa, Portugal
5
iMED, ULisboa, Research Institute for Medicines, Faculty of Pharmacy, ULisboa, 1749-003 Lisboa, Portugal
6
IBEB, Biophysics and Biomedical Engineering, Faculty of Sciences, ULisboa, Campo Grande, 1749-016 Lisboa, Portugal
*
Author to whom correspondence should be addressed.
Received: 23 January 2018 / Revised: 6 March 2018 / Accepted: 19 March 2018 / Published: 27 March 2018
(This article belongs to the Special Issue Supercritical Fluid Extraction)
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Abstract

Interest in new products from aromatic plants as medical and nutritional compounds is increasing. The aim of this work was to apply different extraction methods, including the use of supercritical carbon dioxide extraction, and to test the antioxidant activity of basil (Ocimum basilicum L.) extracts. In vitro efficacy assessments were performed using enzymatic assays. Essential oil obtained by hydrodistillation and volatile oil obtained from supercritical fluid extraction were analyzed by gas chromatography to quantify components. The total phenolic content in the extracts ranged from 35.5 ± 2.9 to 85.3 ± 8.6 mg of gallic acid equivalents and the total flavonoid content ranged from 35.5 ± 2.9 to 93.3 ± 3.9 micromole catechin equivalents per gram of dry weight of extract. All the extracts showed an antioxidant activity with 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid (ABTS), and the reducing power test. Extracts obtained from methanol had a higher antioxidant capacity per the DPPH test results (IC50 = 3.05 ± 0.36 mg/mL) and the reducing power test assay 306.8 ± 21.8 μmol of trolox equivalents per gram of extract (TE/g) compared with ethanolic or supercritical fluid extracts. However, using the ABTS assay, the extract obtained by supercritical fluid extraction had a higher antioxidant capacity with an IC50 of 1.74 ± 0.05 mg/mL. Finally, the examined extracts showed practically no acetylcholinesterase (AChE) inhibitory capacity and a slight inhibitory activity against tyrosinase. View Full-Text
Keywords: Ocimum basilicum; supercritical fluid extraction; phenolic and flavonoids content; antioxidant activity; in vitro efficacy tests Ocimum basilicum; supercritical fluid extraction; phenolic and flavonoids content; antioxidant activity; in vitro efficacy tests
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Coelho, J.; Veiga, J.; Karmali, A.; Nicolai, M.; Pinto Reis, C.; Nobre, B.; Palavra, A. Supercritical CO2 Extracts and Volatile Oil of Basil (Ocimum basilicum L.) Comparison with Conventional Methods. Separations 2018, 5, 21.

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