Antioxidant Artemisia princeps Extract Enhances the Expression of Filaggrin and Loricrin via the AHR/OVOL1 Pathway
Abstract
:1. Introduction
2. Results
2.1. APE Upregulated Antioxidant NRF2/NQO1 Pathway in Normal Human Epidermal Keratinocytes
2.2. APE-Induced AHR Activation in NHEKs
2.3. APE Induced FLG and LOR Gene Upregulation in an AHR/OVOL1-Dependent Manner
3. Discussion
4. Materials and Methods
4.1. Preparation of APE (Artemisia Princeps Extract)
4.2. Cell Culture
4.3. Cytotoxicity Evaluation
4.4. Real-Time PCR (Polymerase Chain Reaction)
4.5. Immunofluorescence Analysis
4.6. Transfection with siRNA against AHR (Aryl Hydrocarbon Receptor)
4.7. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Conflicts of Interest
References
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Gene | Forward Primer | Reverse Primer |
---|---|---|
ACTB | 5′-TTGTTACAGGAAGTCCCTTGCC-3′ | 5′-ATGCTATCACCTCCCCTGTGTG-3′ |
CYP1A1 | 5′-TAGACACTGATCTGGCTGCAG-3′ | 5′-GGGAAGGCTCCATCAGCATC-3′ |
NQO1 | 5′-GGATTGGACCGAGCTGGAA-3′ | 5′-AATTGCAGTGAAGATGAAGGCAAC-3′ |
HO1 | 5′-AAGACTGCGTTCCTGCTCAAC-3′ | 5′-AAAGCCCTACAGCAACTGTCG-3′ |
FLG | 5′-CATGGCAGCTATGGTAGTGCAGA-3′ | 5′-ACCAAACGCACTTGCTTTACAGA-3′ |
LOR | 5′-GGCTGCATCTAGTTCTGCTGTTTA-3′ | 5′-CAAATTTATTGACTGAGGCACTGG-3′ |
OVOL1 | 5′-ACGATGCCCATCCACTACCTG-3′ | 5′-TTTCTGAGGTGCTGGTCATCATTC-3′ |
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Hirano, A.; Goto, M.; Mitsui, T.; Hashimoto-Hachiya, A.; Tsuji, G.; Furue, M. Antioxidant Artemisia princeps Extract Enhances the Expression of Filaggrin and Loricrin via the AHR/OVOL1 Pathway. Int. J. Mol. Sci. 2017, 18, 1948. https://doi.org/10.3390/ijms18091948
Hirano A, Goto M, Mitsui T, Hashimoto-Hachiya A, Tsuji G, Furue M. Antioxidant Artemisia princeps Extract Enhances the Expression of Filaggrin and Loricrin via the AHR/OVOL1 Pathway. International Journal of Molecular Sciences. 2017; 18(9):1948. https://doi.org/10.3390/ijms18091948
Chicago/Turabian StyleHirano, Akiko, Masashi Goto, Tsukasa Mitsui, Akiko Hashimoto-Hachiya, Gaku Tsuji, and Masutaka Furue. 2017. "Antioxidant Artemisia princeps Extract Enhances the Expression of Filaggrin and Loricrin via the AHR/OVOL1 Pathway" International Journal of Molecular Sciences 18, no. 9: 1948. https://doi.org/10.3390/ijms18091948
APA StyleHirano, A., Goto, M., Mitsui, T., Hashimoto-Hachiya, A., Tsuji, G., & Furue, M. (2017). Antioxidant Artemisia princeps Extract Enhances the Expression of Filaggrin and Loricrin via the AHR/OVOL1 Pathway. International Journal of Molecular Sciences, 18(9), 1948. https://doi.org/10.3390/ijms18091948