Mdivi-1 Protects Adult Rat Hippocampal Neural Stem Cells against Palmitate-Induced Oxidative Stress and Apoptosis
Abstract
:1. Introduction
2. Results
2.1. Mdivi-1 Protects Hippocampal NSCs against Palmitate Insult
2.2. Mdivi-1 Inhibits Palmitate-Induced Drp1 Expression and Mitochondrial Translocation
2.3. Mdivi-1 Inhibits Palmitate-Induced Increase in ROS Generation
2.4. Mdivi-1 Stabilizes Mitochondrial Transmembrane Potential (ΔψM)
2.5. Mdivi-1 Increases Bcl-2 and Decreases Bax, Thereby Increasing the Bcl-2/Bax Ratio
2.6. Mdivi-1 Inhibits Palmitate-Induced Increase in Cytochrome C Release and Caspase-3 Activation
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Adult Rat Hippocampal NSCs Cultures and Treatment
4.3. Detection of Cell Death and Apoptosis
4.4. Detection of Intracellular and Mitochondrial ROS Production
4.5. Assessment of Mitochondrial Membrane Potential (ΔψM)
4.6. Western Blot Analysis
4.7. Immunocytochemical Detection of Drp1 or Bax Translocation and Cytochrome C Release
4.8. Statistical Analysis
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
Abbreviations
NSCs | Neural stem cells |
ROS | Reactive oxygen species |
FFA | Free fatty acid |
Drp1 | Dynamin-regulated protein 1 |
BSA | Bovine serum albumin |
DCFDA | 2′,7′-Dichlorodihydrofluorescein diacetate |
JC-1 | 5,5′,6,6′-Tetrachloro-1,1′,3′,3′-tetraethylbenzimidazolylcarbocyanine iodide |
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Kim, S.; Kim, C.; Park, S. Mdivi-1 Protects Adult Rat Hippocampal Neural Stem Cells against Palmitate-Induced Oxidative Stress and Apoptosis. Int. J. Mol. Sci. 2017, 18, 1947. https://doi.org/10.3390/ijms18091947
Kim S, Kim C, Park S. Mdivi-1 Protects Adult Rat Hippocampal Neural Stem Cells against Palmitate-Induced Oxidative Stress and Apoptosis. International Journal of Molecular Sciences. 2017; 18(9):1947. https://doi.org/10.3390/ijms18091947
Chicago/Turabian StyleKim, Sehee, Chanyang Kim, and Seungjoon Park. 2017. "Mdivi-1 Protects Adult Rat Hippocampal Neural Stem Cells against Palmitate-Induced Oxidative Stress and Apoptosis" International Journal of Molecular Sciences 18, no. 9: 1947. https://doi.org/10.3390/ijms18091947