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Open AccessArticle

Analysis and Anticancer Effects of Active Compounds from Spatholobi Caulis in Human Breast Cancer Cells

1
Department of Preventive medicine, College of Korean Medicine, Gachon University, Seongnam 13120, Korea
2
Department of Korean International Medicine, College of Korean Medicine, Gachon University, Seongnam 13120, Korea
3
Department of Life Science, College of Bio-Nano Technology, Gachon University, Seongnam 13120, Korea
4
Department of Physiology, College of Korean Medicine, Gachon University, Seongnam 13120, Korea
5
Herbal Medicine Research Division, Korea Institute of Oriental Medicine, Daejeon 34054, Korea
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Processes 2020, 8(9), 1193; https://doi.org/10.3390/pr8091193
Received: 21 July 2020 / Revised: 18 September 2020 / Accepted: 18 September 2020 / Published: 21 September 2020
(This article belongs to the Special Issue Metabolic Analysis in Food Processing)
Breast cancer is the most common malignancy in both developing and developed countries. In this study, we simultaneously analyzed nine constituent compounds from Spatholobi Caulis (gallic acid, (−)-gallocatechin, 3,4-dihydroxybenzoic acid, procyanidin B1, 3,4-dihydroxybenzaldehyde, catechin, procyanidin B2, epicatechin, and (−)-epicatechin gallate) and examined their anticancer effects on MCF-7 and MDA-MB-231 human breast cancer cells. The experimental results indicated that the gallic acid showed the strongest cytotoxic effect on MCF-7 cells among tested compounds whilst most of samples did not express inhibitory effect on viability of MDA-MB-231 cells, except for 70% ethanol extract of S. Caulis. Thus, gallic acid was chosen to extend anticancer mechanism study on MCF-7 cells. Our data showed that the gallic acid induced apoptotic MCF-7 cell death through both extrinsic and intrinsic pathways, which increased the expression of cleaved caspase-7, -8, and -9, Bax and p53, but reduced the expression of Bcl-2 and poly (ADP-ribose) polymerase (PARP). In addition, the network pharmacological analysis pointed out that the p53, mitogen-activated protein kinase (MAPK), estrogen, and Wnt signaling pathways have a great correlation with the targets of gallic acid. This study suggested that gallic acid is a bioactive component of S. Caulis with potential to be used in chemotherapy for breast cancer. View Full-Text
Keywords: MCF-7; breast cancer; Spatholobi Caulis; gallic acid; apoptosis; p53; network pharmacology MCF-7; breast cancer; Spatholobi Caulis; gallic acid; apoptosis; p53; network pharmacology
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MDPI and ACS Style

Phung, H.M.; Lee, H.; Lee, S.; Jang, D.; Kim, C.-E.; Kang, K.S.; Seo, C.-S.; Choi, Y.-K. Analysis and Anticancer Effects of Active Compounds from Spatholobi Caulis in Human Breast Cancer Cells. Processes 2020, 8, 1193.

AMA Style

Phung HM, Lee H, Lee S, Jang D, Kim C-E, Kang KS, Seo C-S, Choi Y-K. Analysis and Anticancer Effects of Active Compounds from Spatholobi Caulis in Human Breast Cancer Cells. Processes. 2020; 8(9):1193.

Chicago/Turabian Style

Phung, Hung M.; Lee, Hesol; Lee, Sullim; Jang, Dongyeop; Kim, Chang-Eop; Kang, Ki S.; Seo, Chang-Seob; Choi, You-Kyung. 2020. "Analysis and Anticancer Effects of Active Compounds from Spatholobi Caulis in Human Breast Cancer Cells" Processes 8, no. 9: 1193.

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