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Open AccessFeature PaperArticle

TLC-Densitometric Determination of Five Coxibs in Pharmaceutical Preparations

Department of Inorganic and Analytical Chemistry, Faculty of Pharmacy, Jagiellonian University Medical College, 9 Medyczna St., 30-688 Kraków, Poland
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Processes 2020, 8(5), 620; https://doi.org/10.3390/pr8050620
Received: 30 April 2020 / Revised: 17 May 2020 / Accepted: 18 May 2020 / Published: 22 May 2020
A class of drugs called coxibs (COX-2 inhibitors) were created to help relieve pain and inflammation of osteoarthritis and rheumatoid arthritis with the lowest amount of side effects possible. The presented paper describes a new developed, optimized and validated thin layer chromatographic (TLC)-densitometric procedure for the simultaneous assay of five coxibs: celecoxib, etoricoxib, firecoxib, rofecoxib and cimicoxib. Chromatographic separation was conducted on HPTLC F254 silica gel chromatographic plates as a stationary phase using chloroform–acetone–toluene (12:5:2, v/v/v) as a mobile phase. Densitometric detection was carried out at two wavelengths of 254 and 290 nm. The method was tested according to ICH guidelines for linearity, recovery and specificity. The presented method was linear in a wide range of concentrations for all analyzed compounds, with correlation coefficients greater than 0.99. The method is specific, precise (%RSD < 1) and accurate (more than 95%, %RSD < 2). Low-cost, simple and rapid, it can be used in laboratories for drug monitoring and quality control. View Full-Text
Keywords: coxibs; TLC-densitometry; validation of the method; human and veterinary drugs coxibs; TLC-densitometry; validation of the method; human and veterinary drugs
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MDPI and ACS Style

Gumułka, P.; Dąbrowska, M.; Starek, M. TLC-Densitometric Determination of Five Coxibs in Pharmaceutical Preparations. Processes 2020, 8, 620.

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