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Open AccessFeature PaperArticle

VectorDisk: A Microfluidic Platform Integrating Diagnostic Markers for Evidence-Based Mosquito Control

1
Hahn-Schickard, Georges-Koehler-Allee 103, 79110 Freiburg, Germany
2
Laboratory for MEMS Applications, IMTEK—Department of Microsystems Engineering, University of Freiburg, Georges-Koehler-Allee 103, 79110 Freiburg, Germany
3
Swiss Tropical and Public Health Institute, Socinstrasse 57, 4002 Basel, Switzerland
4
University of Basel, Petersplatz 1, 4001 Basel, Switzerland
5
Foundation for Research and Technology-Hellas, Institute of Molecular Biology and Biotechnology, 70013 Heraklion, Greece
6
Pesticide Science Laboratory, Department of Crop Science, Agricultural University of Athens, 11855 Athens, Greece
*
Author to whom correspondence should be addressed.
These authors have equally contributed.
Processes 2020, 8(12), 1677; https://doi.org/10.3390/pr8121677
Received: 24 November 2020 / Revised: 14 December 2020 / Accepted: 15 December 2020 / Published: 18 December 2020
(This article belongs to the Special Issue Advances in Microfluidics Technology for Diagnostics and Detection)
Effective mosquito monitoring relies on the accurate identification and characterization of the target population. Since this process requires specialist knowledge and equipment that is not widely available, automated field-deployable systems are highly desirable. We present a centrifugal microfluidic cartridge, the VectorDisk, which integrates TaqMan PCR assays in two feasibility studies, aiming to assess multiplexing capability, specificity, and reproducibility in detecting disk-integrated vector-related assays. In the first study, pools of 10 mosquitoes were used as samples. We tested 18 disks with 27 DNA and RNA assays each, using a combination of multiple microfluidic chambers and detection wavelengths (geometric and color multiplexing) to identify mosquito and malaria parasite species as well as insecticide resistance mechanisms. In the second study, purified nucleic acids served as samples to test arboviral and malaria infective mosquito assays. Nine disks were tested with 14 assays each. No false positive results were detected on any of the disks. The coefficient of variation in reproducibility tests was <10%. The modular nature of the platform, the easy adaptation of the primer/probe panels, the cold chain independence, the rapid (2–3 h) analysis, and the assay multiplexing capacity are key features, rendering the VectorDisk a potential candidate for automated vector analysis. View Full-Text
Keywords: microfluidics; LabDisk; vector-borne diseases; malaria; arboviruses; insecticide resistances; mosquito monitoring microfluidics; LabDisk; vector-borne diseases; malaria; arboviruses; insecticide resistances; mosquito monitoring
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MDPI and ACS Style

Hin, S.; Baumgartner, D.; Specht, M.; Lüddecke, J.; Mahmodi Arjmand, E.; Johannsen, B.; Schiedel, L.; Rombach, M.; Paust, N.; von Stetten, F.; Zengerle, R.; Wipf, N.; Müller, P.; Mavridis, K.; Vontas, J.; Mitsakakis, K. VectorDisk: A Microfluidic Platform Integrating Diagnostic Markers for Evidence-Based Mosquito Control. Processes 2020, 8, 1677. https://doi.org/10.3390/pr8121677

AMA Style

Hin S, Baumgartner D, Specht M, Lüddecke J, Mahmodi Arjmand E, Johannsen B, Schiedel L, Rombach M, Paust N, von Stetten F, Zengerle R, Wipf N, Müller P, Mavridis K, Vontas J, Mitsakakis K. VectorDisk: A Microfluidic Platform Integrating Diagnostic Markers for Evidence-Based Mosquito Control. Processes. 2020; 8(12):1677. https://doi.org/10.3390/pr8121677

Chicago/Turabian Style

Hin, Sebastian; Baumgartner, Desirée; Specht, Mara; Lüddecke, Jan; Mahmodi Arjmand, Ehsan; Johannsen, Benita; Schiedel, Larissa; Rombach, Markus; Paust, Nils; von Stetten, Felix; Zengerle, Roland; Wipf, Nadja; Müller, Pie; Mavridis, Konstantinos; Vontas, John; Mitsakakis, Konstantinos. 2020. "VectorDisk: A Microfluidic Platform Integrating Diagnostic Markers for Evidence-Based Mosquito Control" Processes 8, no. 12: 1677. https://doi.org/10.3390/pr8121677

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