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Open AccessFeature PaperArticle

Real-Time Nanoplasmonic Sensor for IgG Monitoring in Bioproduction

1
Laboratory of Molecular Materials, Division of Biophysics and Bioengineering, Department of Physics, Chemistry and Biology, Linköping University, 581 83 Linköping, Sweden
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Laboratory of Biotechnology, Division of Biophysics and Bioengineering, Department of Physics, Chemistry and Biology, Linköping University, 581 83 Linköping, Sweden
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Sensor and Actuator Systems, Department of Physics, Chemistry and Biology, Linköping University, 581 83 Linköping, Sweden
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ArgusEye AB, Spannmålsgatan 55, 583 36 Linköping, Sweden
*
Author to whom correspondence should be addressed.
Processes 2020, 8(10), 1302; https://doi.org/10.3390/pr8101302
Received: 21 September 2020 / Revised: 6 October 2020 / Accepted: 14 October 2020 / Published: 16 October 2020
(This article belongs to the Special Issue Measurement Technologies for up- and Downstream Bioprocessing)
Real-time monitoring of product titers during process development and production of biotherapeutics facilitate implementation of quality-by-design principles and enable rapid bioprocess decision and optimization of the production process. Conventional analytical methods are generally performed offline/at-line and, therefore, are not capable of generating real-time data. In this study, a novel fiber optical nanoplasmonic sensor technology was explored for rapid IgG titer measurements. The sensor combines localized surface plasmon resonance transduction and robust single use Protein A-modified sensor chips, housed in a flexible flow cell, for specific IgG detection. The sensor requires small sample volumes (1–150 µL) and shows a reproducibility and sensitivity comparable to Protein G high performance liquid chromatography-ultraviolet (HPLC-UV). The dynamic range of the sensor system can be tuned by varying the sample volume, which enables quantification of IgG samples ranging from 0.0015 to 10 mg/mL, without need for sample dilution. The sensor shows limited interference from the sample matrix and negligible unspecific protein binding. IgG titers can be rapidly determined in samples from filtered unpurified Chinese hamster ovary (CHO) cell cultures and show good correlation with enzyme-linked immunosorbent assay (ELISA). View Full-Text
Keywords: PAT; IgG titer; real-time; on-line; bioprocess; nanoplasmonic PAT; IgG titer; real-time; on-line; bioprocess; nanoplasmonic
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Tran, T.; Eskilson, O.; Mayer, F.; Gustavsson, R.; Selegård, R.; Lundström, I.; Mandenius, C.-F.; Martinsson, E.; Aili, D. Real-Time Nanoplasmonic Sensor for IgG Monitoring in Bioproduction. Processes 2020, 8, 1302.

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