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From the first issue of 2016, Chromatography has changed its name to Separations.

Open AccessReview

Monoliths in Bioprocess Technology

Research Division Biochemical Engineering, Institute of Chemical Engineering, Vienna University of Technology, Gumpendorfer Strasse 1A/166-4, 1060 Vienna, Austria
Christian Doppler Laboratory for Mechanistic and Physiological Methods for Improved Bioprocesses, Institute of Chemical Engineering, Vienna University of Technology, Getreidemarkt 9/166, A-1060 Vienna, Austria
Author to whom correspondence should be addressed.
Academic Editor: Zuzana Zajickov
Chromatography 2015, 2(2), 195-212;
Received: 6 February 2015 / Revised: 8 April 2015 / Accepted: 14 April 2015 / Published: 17 April 2015
(This article belongs to the Special Issue Monolithic Columns in Separation Sciences)
PDF [1712 KB, uploaded 17 April 2015]


Monolithic columns are a special type of chromatography column, which can be used for the purification of different biomolecules. They have become popular due to their high mass transfer properties and short purification times. Several articles have already discussed monolith manufacturing, as well as monolith characteristics. In contrast, this review focuses on the applied aspect of monoliths and discusses the most relevant biomolecules that can be successfully purified by them. We describe success stories for viruses, nucleic acids and proteins and compare them to conventional purification methods. Furthermore, the advantages of monolithic columns over particle-based resins, as well as the limitations of monoliths are discussed. With a compilation of commercially available monolithic columns, this review aims at serving as a ‘yellow pages’ for bioprocess engineers who face the challenge of purifying a certain biomolecule using monoliths. View Full-Text
Keywords: monolith; pDNA purification; virus purification; protein purification; commercially available monolith monolith; pDNA purification; virus purification; protein purification; commercially available monolith

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Rajamanickam, V.; Herwig, C.; Spadiut, O. Monoliths in Bioprocess Technology. Chromatography 2015, 2, 195-212.

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