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Chromatography 2015, 2(2), 188-194; https://doi.org/10.3390/chromatography2020188
Effects of Different Levels of Echinostoma caproni Miracidial Dose on Glucose and Maltose Composition of Biomphalaria glabrata Snails as Determined by High Performance Thin-Layer Chromatography-Densitometry
1
Department of Chemistry, Lafayette College, Easton, PA 18042, USA
2
Department of Biology, Lafayette College, Easton, PA 18042, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Mark Devlin Maloney
Received: 9 March 2015 / Revised: 25 March 2015 / Accepted: 31 March 2015 / Published: 9 April 2015
(This article belongs to the Special Issue New Trends in Thin-Layer Chromatography)
Abstract
The effects of 5, 25, and 40 Echinostoma caproni miracidia on the sugar content of young adult and mature adult Biomphalaria glabrata were studied using high performance thin layer chromatography (HPTLC)-densitometry. Analysis was done on the snail’s digestive gland gonad complex (DGG) at two and four weeks postmiracidial exposure. The sugars were extracted from the DGG using 70% ethanol and analyzed on silica gel HPTLC plates with a preadsorbent zone using 1-butanol-glacial acetic acid-diethyl ether-deionized water (27:18:5:3) mobile phase. The separated bands were then detected using alpha-naphthol-sulfuric reagent and quantified by densitometry at 515 nm. Significant differences were found in the maltose content between two and four weeks post exposure for both age groups. Additionally, significantly lower maltose and glucose levels were observed in the high exposure groups of both ages. View Full-TextKeywords:
Biomphalaria glabrata; Echinostoma caproni; miracidia; digestive-gland gonad complex; DGG; glucose; maltose; sugars; thin-layer chromatography
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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DeGrandchamp, D.; Hartlaub, S.; Fried, B.; Sherma, J. Effects of Different Levels of Echinostoma caproni Miracidial Dose on Glucose and Maltose Composition of Biomphalaria glabrata Snails as Determined by High Performance Thin-Layer Chromatography-Densitometry. Chromatography 2015, 2, 188-194.
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Chromatography
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