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Open AccessArticle

Association between Serum Zinc and Calcification Propensity (T50) in Patients with Type 2 Diabetes Mellitus and In Vitro Effect of Exogenous Zinc on T50

1
Department of Metabolism, Endocrinology and Molecular Medicine, Osaka City University Graduate School of Medicine, 1-4-3 Asahi-machi, Abeno-ku, Osaka 545-8585, Japan
2
Department of Nephrology, Osaka City University Graduate School of Medicine, 1-4-3 Asahi-machi, Abeno-ku, Osaka 545-8585, Japan
3
Division of Internal Medicine, Inoue Hospital, 16-17 enoki-machi, Osaka 564-0053, Japan
4
Department of Vascular Medicine, Osaka City University Graduate School of Medicine, 1-4-3 Asahi-machi, Osaka 545-8585, Japan
5
Vascular Science Center for Translational Research, Osaka City University Graduate School of Medicine, 1-4-3 Asahi-machi, Abeno-ku, Osaka 545-8585, Japan
*
Author to whom correspondence should be addressed.
Biomedicines 2020, 8(9), 337; https://doi.org/10.3390/biomedicines8090337
Received: 31 August 2020 / Revised: 6 September 2020 / Accepted: 8 September 2020 / Published: 9 September 2020
(This article belongs to the Section Molecular and Translational Medicine)
Zinc inhibits vascular calcification in vivo and in vitro. Patients with type 2 diabetes mellitus show hypozincemia and are at an elevated risk of cardiovascular events. Recently, an in vitro test (T50-test) was developed for determination of serum calcification propensity and a shorter T50 means a higher calcification propensity. This cross-sectional study investigated the association between serum zinc and T50 in 132 type 2 diabetes mellitus patients with various kidney functions. Furthermore, the effect of exogenous zinc on T50 was also investigated in vitro using separately pooled serum samples obtained from healthy volunteers and patients with hemodialysis. We measured T50 levels using the established nephelometric method. The median (interquartile range) levels of T50 and serum zinc were 306 (269 to 332) min, and 80.0 (70.1 to 89.8) µg/dL, respectively. Serum zinc level showed a weak, but positive correlation with T50 (rs = 0.219, p = 0.012). This association remained significant in multivariable-adjusted analysis, and was independent of known factors including phosphate, calcium, and magnesium. Kidney function and glycemic control were not significantly associated with T50. Finally, in vitro experiments showed that addition of a physiological concentration of exogenous zinc chloride significantly increased serum T50. Our results indicate that serum zinc is an independent factor with a potential role in suppressing calcification propensity in serum. View Full-Text
Keywords: serum calcification propensity; zinc; type 2 diabetes mellitus serum calcification propensity; zinc; type 2 diabetes mellitus
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MDPI and ACS Style

Nakatani, S.; Mori, K.; Sonoda, M.; Nishide, K.; Uedono, H.; Tsuda, A.; Emoto, M.; Shoji, T. Association between Serum Zinc and Calcification Propensity (T50) in Patients with Type 2 Diabetes Mellitus and In Vitro Effect of Exogenous Zinc on T50. Biomedicines 2020, 8, 337. https://doi.org/10.3390/biomedicines8090337

AMA Style

Nakatani S, Mori K, Sonoda M, Nishide K, Uedono H, Tsuda A, Emoto M, Shoji T. Association between Serum Zinc and Calcification Propensity (T50) in Patients with Type 2 Diabetes Mellitus and In Vitro Effect of Exogenous Zinc on T50. Biomedicines. 2020; 8(9):337. https://doi.org/10.3390/biomedicines8090337

Chicago/Turabian Style

Nakatani, Shinya; Mori, Katsuhito; Sonoda, Mika; Nishide, Kozo; Uedono, Hideki; Tsuda, Akihiro; Emoto, Masanori; Shoji, Tetsuo. 2020. "Association between Serum Zinc and Calcification Propensity (T50) in Patients with Type 2 Diabetes Mellitus and In Vitro Effect of Exogenous Zinc on T50" Biomedicines 8, no. 9: 337. https://doi.org/10.3390/biomedicines8090337

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