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Chemosensors 2018, 6(3), 33; https://doi.org/10.3390/chemosensors6030033

Label-Free Biosensing Method for the Detection of a Pancreatic Cancer Biomarker Based on Dielectrophoresis Spectroscopy

Department of Electrical and Computer Engineering, North Dakota State University, Fargo, ND 58108-6050, USA
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Received: 9 July 2018 / Revised: 7 August 2018 / Accepted: 7 August 2018 / Published: 11 August 2018
(This article belongs to the Special Issue Label-Free Biosensors and Chemical Sensors)
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Abstract

We show that negative dielectrophoresis (DEP) spectroscopy is an effective transduction mechanism of a biosensor for the diagnosis and prognosis of pancreatic cancer using the biomarker CA 19-9. A substantial change in the negative DEP force applied to functionalized polystyrene microspheres (PM) was observed with respect to both the concentration level of the pancreatic cancer biomarker CA 19-9 and the frequency of the electric field produced by a pearl shaped interdigitated gold micro-electrode. The velocity of repulsion of a set of PM functionalized to a monoclonal antibody to CA 19-9 was calculated for several concentration cutoff levels of CA 19-9, including 0 U/mL and 37 U/mL, at the frequency range from 0.5 to 2 MHz. The velocity of repulsion of the PM from the electrode was determined using a side illumination and an automated software using a real-time image processing technique that captures the Mie scattering from the PM. Since negative DEP spectroscopy is an effective transduction mechanism for the detection of the cutoff levels of CA 19-9, it has the potential to be used in the early stage diagnosis and in the prognosis of pancreatic cancer. View Full-Text
Keywords: bioelectronics; dielectrophoresis; pancreatic cancer; label free; spectroscopy bioelectronics; dielectrophoresis; pancreatic cancer; label free; spectroscopy
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Dackson Gudagunti, F.; Velmanickam, L.; Nawarathna, D.; Lima, I.T., Jr. Label-Free Biosensing Method for the Detection of a Pancreatic Cancer Biomarker Based on Dielectrophoresis Spectroscopy. Chemosensors 2018, 6, 33.

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