A Low-Cost Label-Free AFB1 Impedimetric Immunosensor Based on Functionalized CD-Trodes

Foguel, Marcos Vinicius; Furlan Giordano, Gabriela; De Sylos, Célia Maria; Carlos, Iracilda Zeppone; Pupim Ferreira, Antonio Aparecido; Benedetti, Assis Vicente; Yamanaka, Hideko

doi:10.3390/chemosensors4030017

Open AccessTechnical Note

A Low-Cost Label-Free AFB₁ Impedimetric Immunosensor Based on Functionalized CD-Trodes

by

Marcos Vinicius Foguel

^1,*,

Gabriela Furlan Giordano

¹,

Célia Maria De Sylos

²,

Iracilda Zeppone Carlos

³,

Antonio Aparecido Pupim Ferreira

¹,

Assis Vicente Benedetti

¹ and

Hideko Yamanaka

^1,*

¹

Department of Analytical Chemistry, Institute of Chemistry, UNESP-Univ Estadual Paulista, Rua Francisco Degni, 55, Quitandinha, 14800-060 Araraquara, SP, Brazil

²

Department of Food and Nutrition, School of Pharmaceutical Sciences, UNESP-Univ Estadual Paulista, Rodovia Araraquara Jaú, Km 01, s/n, Campos Ville, 14800-903 Araraquara, SP, Brazil

³

Department of Clinical Analysis, School of Pharmaceutical Sciences, UNESP-Univ Estadual Paulista, Rodovia Araraquara Jaú, Km 01, s/n, Campos Ville, 14800-903 Araraquara, SP, Brazil

^*

Authors to whom correspondence should be addressed.

Chemosensors 2016, 4(3), 17; https://doi.org/10.3390/chemosensors4030017

Submission received: 29 April 2016 / Revised: 22 June 2016 / Accepted: 19 August 2016 / Published: 1 September 2016

(This article belongs to the Special Issue Electrochemical Immunosensors and Aptasensors)

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Abstract

This work describes the investigation of a label-free immunosensor for the detection of aflatoxin B₁ (AFB₁). CD-trodes (electrodes obtained from recordable compact disks) were used as low-cost and disposable transducers after modification with a self-assembled monolayer (SAM) of lipoic acid. The anti-aflatoxin B₁ antibody was immobilized via EDC/NHS activation, followed by blocking with bovine serum albumin and immunoassays with AFB₁. The optimization of analytical parameters and the detection were carried out using electrochemical impedance measurements. Using chemometric tools, the best conditions for the immunosensor development were defined as: anti-AFB₁ antibody at 1:2000 dilution and surface blocking with 0.5% bovine serum albumin, both incubated for 1 h, and antibody–antigen immunoreaction for 30 min. The impedimetric immunosensor showed a linear range from 5 × 10⁻⁹ to 1 × 10⁻⁷ mol·L⁻¹ (1.56–31.2 ng·mL⁻¹), limit of detection and limit of quantification, respectively, 3.6 × 10⁻¹⁰ and 1.1 × 10⁻⁹mol·L⁻¹ (0.11 and 0.34 ng·mL⁻¹). The proposed immunosensor was applied to analyze peanut samples.

Keywords: aflatoxin B₁; immunosensor; electrochemical impedance spectroscopy

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MDPI and ACS Style

Foguel, M.V.; Furlan Giordano, G.; De Sylos, C.M.; Carlos, I.Z.; Pupim Ferreira, A.A.; Benedetti, A.V.; Yamanaka, H. A Low-Cost Label-Free AFB₁ Impedimetric Immunosensor Based on Functionalized CD-Trodes. Chemosensors 2016, 4, 17. https://doi.org/10.3390/chemosensors4030017

AMA Style

Foguel MV, Furlan Giordano G, De Sylos CM, Carlos IZ, Pupim Ferreira AA, Benedetti AV, Yamanaka H. A Low-Cost Label-Free AFB₁ Impedimetric Immunosensor Based on Functionalized CD-Trodes. Chemosensors. 2016; 4(3):17. https://doi.org/10.3390/chemosensors4030017

Chicago/Turabian Style

Foguel, Marcos Vinicius, Gabriela Furlan Giordano, Célia Maria De Sylos, Iracilda Zeppone Carlos, Antonio Aparecido Pupim Ferreira, Assis Vicente Benedetti, and Hideko Yamanaka. 2016. "A Low-Cost Label-Free AFB₁ Impedimetric Immunosensor Based on Functionalized CD-Trodes" Chemosensors 4, no. 3: 17. https://doi.org/10.3390/chemosensors4030017

APA Style

Foguel, M. V., Furlan Giordano, G., De Sylos, C. M., Carlos, I. Z., Pupim Ferreira, A. A., Benedetti, A. V., & Yamanaka, H. (2016). A Low-Cost Label-Free AFB₁ Impedimetric Immunosensor Based on Functionalized CD-Trodes. Chemosensors, 4(3), 17. https://doi.org/10.3390/chemosensors4030017

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A Low-Cost Label-Free AFB₁ Impedimetric Immunosensor Based on Functionalized CD-Trodes

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