Next Article in Journal
Fungi Active Microbial Metabolism Detection of Rhizopus sp. and Aspergillus sp. Section Nigri on Strawberry Using a Set of Chemical Sensors Based on Carbon Nanostructures
Next Article in Special Issue
Recent Trends in Field-Effect Transistors-Based Immunosensors
Previous Article in Journal / Special Issue
A Low-Cost Label-Free AFB1 Impedimetric Immunosensor Based on Functionalized CD-Trodes
Article Menu

Export Article

Open AccessArticle
Chemosensors 2016, 4(3), 18;

Miniaturized Aptamer-Based Assays for Protein Detection

Elettra-Sincrotone S.C.p.A., SS 14 km 163,5 in AREA Science Park, Basovizza, Trieste 34149, Italy
Department of Physics, University of Trieste, Via Valerio 9, Trieste 34127, Italy
INSTM-ST Unit, SS 14 km 163,5 in AREA Science Park, Basovizza, Trieste 34149, Italy
Centre of Excellence for Biosensors, Instrumentation and Process Control, Tovarniška 26, Ajdovščina SI-5270, Slovenia
Author to whom correspondence should be addressed.
Academic Editors: Paolo Ugo and Ligia Moretto
Received: 11 July 2016 / Revised: 12 August 2016 / Accepted: 29 August 2016 / Published: 2 September 2016
(This article belongs to the Special Issue Electrochemical Immunosensors and Aptasensors)
PDF [3255 KB, uploaded 2 September 2016]


The availability of devices for cancer biomarker detection at early stages of the disease is one of the most critical issues in biomedicine. Towards this goal, to increase the assay sensitivity, device miniaturization strategies empowered by the employment of high affinity protein binders constitute a valuable approach. In this work we propose two different surface-based miniaturized platforms for biomarker detection in body fluids: the first platform is an atomic force microscopy (AFM)-based nanoarray, where AFM is used to generate functional nanoscale areas and to detect biorecognition through careful topographic measurements; the second platform consists of a miniaturized electrochemical cell to detect biomarkers through electrochemical impedance spectroscopy (EIS) analysis. Both devices rely on robust and highly-specific protein binders as aptamers, and were tested for thrombin detection. An active layer of DNA-aptamer conjugates was immobilized via DNA directed immobilization on complementary single-stranded DNA self-assembled monolayers confined on a nano/micro area of a gold surface. Results obtained with these devices were compared with the output of surface plasmon resonance (SPR) assays used as reference. We succeeded in capturing antigens in concentrations as low as a few nM. We put forward ideas to push the sensitivity further to the pM range, assuring low biosample volume (μL range) assay conditions. View Full-Text
Keywords: biosensors; aptamers; AFM; nanoarray; EIS biosensors; aptamers; AFM; nanoarray; EIS

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material

Printed Edition Available!
A printed edition of this Special Issue is available here.

Share & Cite This Article

MDPI and ACS Style

Bosco, A.; Ambrosetti, E.; Mavri, J.; Capaldo, P.; Casalis, L. Miniaturized Aptamer-Based Assays for Protein Detection. Chemosensors 2016, 4, 18.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Chemosensors EISSN 2227-9040 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top