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Miniaturized Aptamer-Based Assays for Protein Detection

by 1,†,‡, 1,2,3,†, 4, 1,2,3 and 1,*
Elettra-Sincrotone S.C.p.A., SS 14 km 163,5 in AREA Science Park, Basovizza, Trieste 34149, Italy
Department of Physics, University of Trieste, Via Valerio 9, Trieste 34127, Italy
INSTM-ST Unit, SS 14 km 163,5 in AREA Science Park, Basovizza, Trieste 34149, Italy
Centre of Excellence for Biosensors, Instrumentation and Process Control, Tovarniška 26, Ajdovščina SI-5270, Slovenia
Author to whom correspondence should be addressed.
Academic Editors: Paolo Ugo and Ligia Moretto
Chemosensors 2016, 4(3), 18;
Received: 11 July 2016 / Revised: 12 August 2016 / Accepted: 29 August 2016 / Published: 2 September 2016
(This article belongs to the Special Issue Electrochemical Immunosensors and Aptasensors)
The availability of devices for cancer biomarker detection at early stages of the disease is one of the most critical issues in biomedicine. Towards this goal, to increase the assay sensitivity, device miniaturization strategies empowered by the employment of high affinity protein binders constitute a valuable approach. In this work we propose two different surface-based miniaturized platforms for biomarker detection in body fluids: the first platform is an atomic force microscopy (AFM)-based nanoarray, where AFM is used to generate functional nanoscale areas and to detect biorecognition through careful topographic measurements; the second platform consists of a miniaturized electrochemical cell to detect biomarkers through electrochemical impedance spectroscopy (EIS) analysis. Both devices rely on robust and highly-specific protein binders as aptamers, and were tested for thrombin detection. An active layer of DNA-aptamer conjugates was immobilized via DNA directed immobilization on complementary single-stranded DNA self-assembled monolayers confined on a nano/micro area of a gold surface. Results obtained with these devices were compared with the output of surface plasmon resonance (SPR) assays used as reference. We succeeded in capturing antigens in concentrations as low as a few nM. We put forward ideas to push the sensitivity further to the pM range, assuring low biosample volume (μL range) assay conditions. View Full-Text
Keywords: biosensors; aptamers; AFM; nanoarray; EIS biosensors; aptamers; AFM; nanoarray; EIS
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MDPI and ACS Style

Bosco, A.; Ambrosetti, E.; Mavri, J.; Capaldo, P.; Casalis, L. Miniaturized Aptamer-Based Assays for Protein Detection. Chemosensors 2016, 4, 18.

AMA Style

Bosco A, Ambrosetti E, Mavri J, Capaldo P, Casalis L. Miniaturized Aptamer-Based Assays for Protein Detection. Chemosensors. 2016; 4(3):18.

Chicago/Turabian Style

Bosco, Alessandro, Elena Ambrosetti, Jan Mavri, Pietro Capaldo, and Loredana Casalis. 2016. "Miniaturized Aptamer-Based Assays for Protein Detection" Chemosensors 4, no. 3: 18.

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