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Proteomes 2018, 6(4), 51;

Cell-Type-Specific Proteomics: A Neuroscience Perspective

Yale/NIDA Neuroproteomics Center, 300 George St., New Haven, CT 06511, USA
Department of Psychiatry, Yale School of Medicine, Connecticut Mental Health Center, New Haven, CT 06511, USA
Author to whom correspondence should be addressed.
Received: 13 November 2018 / Revised: 4 December 2018 / Accepted: 5 December 2018 / Published: 9 December 2018
(This article belongs to the Special Issue Neuroproteomics)
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Cell-type-specific analysis has become a major focus for many investigators in the field of neuroscience, particularly because of the large number of different cell populations found in brain tissue that play roles in a variety of developmental and behavioral disorders. However, isolation of these specific cell types can be challenging due to their nonuniformity and complex projections to different brain regions. Moreover, many analytical techniques used for protein detection and quantitation remain insensitive to the low amounts of protein extracted from specific cell populations. Despite these challenges, methods to improve proteomic yield and increase resolution continue to develop at a rapid rate. In this review, we highlight the importance of cell-type-specific proteomics in neuroscience and the technical difficulties associated. Furthermore, current progress and technological advancements in cell-type-specific proteomics research are discussed with an emphasis in neuroscience. View Full-Text
Keywords: cell type; neuroscience; proteomics; mass spectrometry; neuron; proximity labeling; affinity chromatography; neuroproteomics; biotinylation cell type; neuroscience; proteomics; mass spectrometry; neuron; proximity labeling; affinity chromatography; neuroproteomics; biotinylation

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Wilson, R.S.; Nairn, A.C. Cell-Type-Specific Proteomics: A Neuroscience Perspective. Proteomes 2018, 6, 51.

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