Next Article in Journal
Proteomic Contributions to Medicinal Plant Research: From Plant Metabolism to Pharmacological Action
Previous Article in Journal
Variety and Dynamics of Proteoforms in the Human Proteome: Aspects of Markers for Hepatocellular Carcinoma
Previous Article in Special Issue
Proteogenomics in Aid of Host–Pathogen Interaction Studies: A Bacterial Perspective
Article Menu

Export Article

Open AccessArticle
Proteomes 2017, 5(4), 34;

Isolation and Characterization of Serum Extracellular Vesicles (EVs) from Atlantic Salmon Infected with Piscirickettsia Salmonis

Department of Animal and Aquacultural Sciences, Norwegian University of Life Sciences, 1433 Aas, Norway
Center of Integrative Microbial Evolution and Department of Pharmaceutical Biosciences, School of Pharmacy, Faculty of Mathematics and Natural Science, University of Oslo, 0316 Oslo, Norway
Department of Food Safety and Infection Biology, Norwegian University of Life Sciences, 0454 Oslo, Norway
Norwegian Veterinary Institute, Pb 750 Sentrum, 0106 Oslo, Norway
Author to whom correspondence should be addressed.
Received: 23 October 2017 / Revised: 21 November 2017 / Accepted: 29 November 2017 / Published: 1 December 2017
(This article belongs to the Special Issue Proteomic Analysis of Host-Microbial Pathogen Interactions)
Full-Text   |   PDF [1871 KB, uploaded 1 December 2017]   |  


Secretion of extracellular vesicles (EVs) is a common feature of both eukaryotic and prokaryotic cells. Isolated EVs have been shown to contain different types of molecules, including proteins and nucleic acids, and are reported to be key players in intercellular communication. Little is known, however, of EV secretion in fish, or the effect of infection on EV release and content. In the present study, EVs were isolated from the serum of healthy and Piscirickettsia salmonis infected Atlantic salmon in order to evaluate the effect of infection on EV secretion. P. salmonis is facultative intracellular bacterium that causes a systemic infection disease in farmed salmonids. EVs isolated from both infected and non-infected fish had an average diameter of 230–300 nm, as confirmed by transmission electron microscopy, nanoparticle tracking, and flow cytometry. Mass spectrometry identified 180 proteins in serum EVs from both groups of fish. Interestingly, 35 unique proteins were identified in serum EVs isolated from the fish infected with P. salmonis. These unique proteins included proteasomes subunits, granulins, and major histocompatibility class I and II. Our results suggest that EV release could be part of a mechanism in which host stimulatory molecules are released from infected cells to promote an immune response. View Full-Text
Keywords: extracellular vesicles (EVs); piscirickettsia salmonis; proteome; immunity extracellular vesicles (EVs); piscirickettsia salmonis; proteome; immunity

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material


Share & Cite This Article

MDPI and ACS Style

Lagos, L.; Tandberg, J.; Kashulin-Bekkelund, A.; Colquhoun, D.J.; Sørum, H.; Winther-Larsen, H.C. Isolation and Characterization of Serum Extracellular Vesicles (EVs) from Atlantic Salmon Infected with Piscirickettsia Salmonis. Proteomes 2017, 5, 34.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Proteomes EISSN 2227-7382 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top