A Microsomal Proteomics View of H2O2- and ABA-Dependent Responses
1
Cambridge Centre for Proteomics, Cambridge Systems Biology Centre, Department of Biochemistry, University of Cambridge Tennis Court Road, Cambridge CB2 1QR, UK
2
Biological and Environmental Sciences & Engineering Division, King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia
3
HM. Clause, rue Louis Saillant, Z.I. La Motte, BP83, 26802 Portes-lès-Valence, France
4
Department of Chemistry, Biology & Biotechnology, University of Perugia, Borgo XX giugno 74, 06121 Perugia, Italy
5
Laboratoire de Physiologie Cellulaire et Végétale, Université Grenoble Alpes, CEA/BIG, 17, avenue des Martyrs, 38054 Grenoble, France
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Proteomes 2017, 5(3), 22; https://doi.org/10.3390/proteomes5030022
Received: 18 May 2017
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Revised: 28 July 2017
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Accepted: 16 August 2017
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Published: 18 August 2017
(This article belongs to the Special Issue Plant Proteomics 2017)
The plant hormone abscisic acid (ABA) modulates a number of plant developmental processes and responses to stress. In planta, ABA has been shown to induce reactive oxygen species (ROS) production through the action of plasma membrane-associated nicotinamide adenine dinucleotide phosphate (NADPH)-oxidases. Although quantitative proteomics studies have been performed to identify ABA- or hydrogen peroxide (H2O2)-dependent proteins, little is known about the ABA- and H2O2-dependent microsomal proteome changes. Here, we examined the effect of 50 µM of either H2O2 or ABA on the Arabidopsis microsomal proteome using tandem mass spectrometry and identified 86 specifically H2O2-dependent, and 52 specifically ABA-dependent proteins that are differentially expressed. We observed differential accumulation of proteins involved in the tricarboxylic acid (TCA) cycle notably in response to H2O2. Of these, aconitase 3 responded to both H2O2 and ABA. Additionally, over 30 proteins linked to RNA biology responded significantly to both treatments. Gene ontology categories such as ‘response to stress’ and ‘transport’ were enriched, suggesting that H2O2 or ABA directly and/or indirectly cause complex and partly overlapping cellular responses. Data are available via ProteomeXchange with identifier PXD006513.
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Keywords:
hydrogen peroxide (H2O2); abscisic acid (ABA); microsomal proteomics; quantitative proteomics; mass spectrometry
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MDPI and ACS Style
Alqurashi, M.; Thomas, L.; Gehring, C.; Marondedze, C. A Microsomal Proteomics View of H2O2- and ABA-Dependent Responses. Proteomes 2017, 5, 22. https://doi.org/10.3390/proteomes5030022
AMA Style
Alqurashi M, Thomas L, Gehring C, Marondedze C. A Microsomal Proteomics View of H2O2- and ABA-Dependent Responses. Proteomes. 2017; 5(3):22. https://doi.org/10.3390/proteomes5030022
Chicago/Turabian StyleAlqurashi, May, Ludivine Thomas, Chris Gehring, and Claudius Marondedze. 2017. "A Microsomal Proteomics View of H2O2- and ABA-Dependent Responses" Proteomes 5, no. 3: 22. https://doi.org/10.3390/proteomes5030022
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