Comparative Proteomic Analysis of Cotton Fiber Development and Protein Extraction Method Comparison in Late Stage Fibers
by
Hana Mujahid 1, Ken Pendarvis 2,†, Joseph S. Reddy 3,‡, Babi Ramesh Reddy Nallamilli 1,§, K. R. Reddy 4, Bindu Nanduri 3 and Zhaohua Peng 1,*
Hana Mujahid 1, Ken Pendarvis 2,†, Joseph S. Reddy 3,‡, Babi Ramesh Reddy Nallamilli 1,§, K. R. Reddy 4, Bindu Nanduri 3 and Zhaohua Peng 1,*
1
Department of Biochemistry, Molecular Biology, Entomology, and Plant Pathology, Mississippi State University, Mississippi State, MS 39762, USA
2
Institute for Genomics, Biocomputing and Biotechnology, Mississippi Agricultural and Forestry Experiment Station, Mississippi State University, Mississippi State, MS 39762, USA
3
College of Veterinary Medicine, Mississippi State University, Mississippi State, MS 39762, USA
4
Department of Plant and Soil Sciences, Mississippi State University, Mississippi State, MS 39762, USA
*
Author to whom correspondence should be addressed.
†
Present Address: College of Agriculture and Life Sciences, University of Arizona, P.O. Box 210036, Tucson, AZ 85721, USA
‡
Present Address: Department of Health Sciences Research, Mayo Clinic, Jacksonville, FL 32224, USA
§
Present Address: Department of Human Genetics, Emory University School of Medicine, Atlanta, GA 30322, USA.
Academic Editors: Ganesh Kumar Agrawal, Sun Tae Kim and Randeep Rakwal
Proteomes 2016, 4(1), 7; https://doi.org/10.3390/proteomes4010007
Received: 20 November 2015 / Revised: 19 January 2016 / Accepted: 28 January 2016 / Published: 3 February 2016
(This article belongs to the Special Issue Advances in Plant Proteomics: Methodology to Biology)
The distinct stages of cotton fiber development and maturation serve as a single-celled model for studying the molecular mechanisms of plant cell elongation, cell wall development and cellulose biosynthesis. However, this model system of plant cell development is compromised for proteomic studies due to a lack of an efficient protein extraction method during the later stages of fiber development, because of a recalcitrant cell wall and the presence of abundant phenolic compounds. Here, we compared the quality and quantities of proteins extracted from 25 dpa (days post anthesis) fiber with multiple protein extraction methods and present a comprehensive quantitative proteomic study of fiber development from 10 dpa to 25 dpa. Comparative analysis using a label-free quantification method revealed 287 differentially-expressed proteins in the 10 dpa to 25 dpa fiber developmental period. Proteins involved in cell wall metabolism and regulation, cytoskeleton development and carbohydrate metabolism among other functional categories in four fiber developmental stages were identified. Our studies provide protocols for protein extraction from maturing fiber tissues for mass spectrometry analysis and expand knowledge of the proteomic profile of cotton fiber development.
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Keywords:
cotton; fiber elongation; cell wall; comparative proteomics; label-free
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MDPI and ACS Style
Mujahid, H.; Pendarvis, K.; Reddy, J.S.; Nallamilli, B.R.R.; Reddy, K.R.; Nanduri, B.; Peng, Z. Comparative Proteomic Analysis of Cotton Fiber Development and Protein Extraction Method Comparison in Late Stage Fibers. Proteomes 2016, 4, 7.
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