Identification of Biomarkers for Resistance to Fusarium oxysporum f. sp. cubense Infection and in Silico Studies in Musa paradisiaca Cultivar Puttabale through Proteomic Approach

Ramu, Venkatesh; Venkatarangaiah, Krishna; Krishnappa, Pradeepa; Shimoga Rajanna, Santosh Kumar; Deeplanaik, Nagaraja; Chandra Pal, Anup; Kini, Kukkundoor Ramachandra

doi:10.3390/proteomes4010009

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Identification of Biomarkers for Resistance to Fusarium oxysporum f. sp. cubense Infection and in Silico Studies in Musa paradisiaca Cultivar Puttabale through Proteomic Approach

Venkatesh Ramu

^1,†,

Krishna Venkatarangaiah

^1,*,

Pradeepa Krishnappa

²,

Santosh Kumar Shimoga Rajanna

¹,

Nagaraja Deeplanaik

¹,

Anup Chandra Pal

³ and

Kukkundoor Ramachandra Kini

Department of Post-Graduate Studies and Research in Biotechnology and Bioinformatics, Kuvempu University, Shankaraghatta 577 451, India

Department of Biotechnology Engineering, M S Ramaiah Institute of Technology, Bangalore 560 054, Karnataka, India

Department of Studies in Biotechnology, University of Mysore, Manasagangotri, Mysore 570 006, India

Author to whom correspondence should be addressed.

^†

Present address: Department of Biochemistry, Indian Institute of Science, Bangalore 560 012, India

Academic Editors: Ganesh Kumar Agrawal, Sun Tae Kim and Randeep Rakwal

Proteomes 2016, 4(1), 9; https://doi.org/10.3390/proteomes4010009

Received: 23 December 2015 / Revised: 12 February 2016 / Accepted: 17 February 2016 / Published: 24 February 2016

(This article belongs to the Special Issue Advances in Plant Proteomics: Methodology to Biology)

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Abstract

Panama wilt caused by Fusarium oxysporum f. sp. cubense (Foc) is one of the major disease constraints of banana production. Previously, we reported the disease resistance Musa paradisiaca cv. puttabale clones developed from Ethylmethanesulfonate and Foc culture filtrate against Foc inoculation. Here, the same resistant clones and susceptible clones were used for the study of protein accumulation against Foc inoculation by two-dimensional gel electrophoresis (2-DE), their expression pattern and an in silico approach. The present investigation revealed mass-spectrometry identified 16 proteins that were over accumulated and 5 proteins that were under accumulated as compared to the control. The polyphosphoinositide binding protein ssh2p (PBPssh2p) and Indoleacetic acid-induced-like (IAA) protein showed significant up-regulation and down-regulation. The docking of the pathogenesis-related protein (PR) with the fungal protein endopolygalacturonase (PG) exemplify the three ionic interactions and seven hydrophobic residues that tends to good interaction at the active site of PG with free energy of assembly dissociation (1.5 kcal/mol). The protein-ligand docking of the Peptide methionine sulfoxide reductase chloroplastic-like protein (PMSRc) with the ligand β-1,3 glucan showed minimum binding energy (−6.48 kcal/mol) and docking energy (−8.2 kcal/mol) with an interaction of nine amino-acid residues. These explorations accelerate the research in designing the host pathogen interaction studies for the better management of diseases. View Full-Text

Keywords: panama wilt; Musa paradisiaca cv. puttabale; PR proteins; two dimensional gel electrophoresis; protein-protein docking

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Ramu, V.; Venkatarangaiah, K.; Krishnappa, P.; Shimoga Rajanna, S.K.; Deeplanaik, N.; Chandra Pal, A.; Kini, K.R. Identification of Biomarkers for Resistance to Fusarium oxysporum f. sp. cubense Infection and in Silico Studies in Musa paradisiaca Cultivar Puttabale through Proteomic Approach. Proteomes 2016, 4, 9. https://doi.org/10.3390/proteomes4010009

AMA Style

Ramu V, Venkatarangaiah K, Krishnappa P, Shimoga Rajanna SK, Deeplanaik N, Chandra Pal A, Kini KR. Identification of Biomarkers for Resistance to Fusarium oxysporum f. sp. cubense Infection and in Silico Studies in Musa paradisiaca Cultivar Puttabale through Proteomic Approach. Proteomes. 2016; 4(1):9. https://doi.org/10.3390/proteomes4010009

Chicago/Turabian Style

Ramu, Venkatesh, Krishna Venkatarangaiah, Pradeepa Krishnappa, Santosh Kumar Shimoga Rajanna, Nagaraja Deeplanaik, Anup Chandra Pal, and Kukkundoor Ramachandra Kini. 2016. "Identification of Biomarkers for Resistance to Fusarium oxysporum f. sp. cubense Infection and in Silico Studies in Musa paradisiaca Cultivar Puttabale through Proteomic Approach" Proteomes 4, no. 1: 9. https://doi.org/10.3390/proteomes4010009

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Identification of Biomarkers for Resistance to Fusarium oxysporum f. sp. cubense Infection and in Silico Studies in Musa paradisiaca Cultivar Puttabale through Proteomic Approach

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