Next Article in Journal
Anti-Cancer Properties of Flaxseed Proteome
Next Article in Special Issue
Quantitative Proteomics Reveal Region-Specific Alterations in Neuroserpin-Deficient Mouse Brain and Retina: Insights into Serpini1 Function
Previous Article in Journal
Benefits of FAIMS to Improve the Proteome Coverage of Deteriorated and/or Cross-Linked TMT 10-Plex FFPE Tissue and Plasma-Derived Exosomes Samples
Previous Article in Special Issue
Comparative Proteomic Analysis of Two Commonly Used Laboratory Yeast Strains: W303 and BY4742
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Proteomes
Volume 11
Issue 4
10.3390/proteomes11040036
proteomes-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Proteome-Wide Profiling Using Sample Multiplexing of a Human Cell Line Treated with Cannabidiol (CBD) and Tetrahydrocannabinol (THC)

by
Morteza Abyadeh
1,†,
Vivek Gupta
2,
Xinyue Liu
3,
Valentina Rossio
3,
Mehdi Mirzaei
2,
Jennifer Cornish
4,
Joao A. Paulo
3 and
Paul A. Haynes
5,*
1
ProGene Technologies Pty Ltd., Macquarie Park, NSW 2113, Australia
2
Macquarie Medical School, Faculty of Medicine, Health and Human Sciences, Macquarie University, Sydney, NSW 2109, Australia
3
Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
4
School of Psychological Sciences, Faculty of Medicine, Health and Human Sciences, Macquarie University, Sydney, NSW 2109, Australia
5
School of Natural Sciences, Macquarie University, North Ryde, NSW 2109, Australia
*
Author to whom correspondence should be addressed.
†
Current address: Department of Biology, Virginia Commonwealth University, Richmond, VA 23284, USA.
Proteomes 2023, 11(4), 36; https://doi.org/10.3390/proteomes11040036
Submission received: 29 August 2023 / Revised: 25 October 2023 / Accepted: 31 October 2023 / Published: 2 November 2023
(This article belongs to the Special Issue Quantitative Proteomics: Techniques and Applications)
Browse Figures
Versions Notes

Abstract

Cannabis has been used historically for both medicinal and recreational purposes, with the most notable cannabinoids being cannabidiol (CBD) and tetrahydrocannabinol (THC). Although their therapeutic effects have been well studied and their recreational use is highly debated, the underlying mechanisms of their biological effects remain poorly defined. In this study, we use isobaric tag-based sample multiplexed proteome profiling to investigate protein abundance differences in the human neuroblastoma SH-SY5Y cell line treated with CBD and THC. We identified significantly regulated proteins by each treatment and performed a pathway classification and associated protein–protein interaction analysis. Our findings suggest that these treatments may lead to mitochondrial dysfunction and induce endoplasmic reticulum stress. These data can potentially be interrogated further to investigate the potential role of CBD and THC in various biological and disease contexts, providing a foundation for future studies.
Keywords: tandem mass tags; TMTpro; real-time database searching; unfolded protein response; high-field asymmetric ion mobility spectrometry; quantitative proteomics; cannabidiol; Delta-9-tetrahydrocannabinol; mitochondrial dysfunction; endoplasmic reticulum stress tandem mass tags; TMTpro; real-time database searching; unfolded protein response; high-field asymmetric ion mobility spectrometry; quantitative proteomics; cannabidiol; Delta-9-tetrahydrocannabinol; mitochondrial dysfunction; endoplasmic reticulum stress

Share and Cite

MDPI and ACS Style

Abyadeh, M.; Gupta, V.; Liu, X.; Rossio, V.; Mirzaei, M.; Cornish, J.; Paulo, J.A.; Haynes, P.A. Proteome-Wide Profiling Using Sample Multiplexing of a Human Cell Line Treated with Cannabidiol (CBD) and Tetrahydrocannabinol (THC). Proteomes 2023, 11, 36. https://doi.org/10.3390/proteomes11040036

AMA Style

Abyadeh M, Gupta V, Liu X, Rossio V, Mirzaei M, Cornish J, Paulo JA, Haynes PA. Proteome-Wide Profiling Using Sample Multiplexing of a Human Cell Line Treated with Cannabidiol (CBD) and Tetrahydrocannabinol (THC). Proteomes. 2023; 11(4):36. https://doi.org/10.3390/proteomes11040036

Chicago/Turabian Style

Abyadeh, Morteza, Vivek Gupta, Xinyue Liu, Valentina Rossio, Mehdi Mirzaei, Jennifer Cornish, Joao A. Paulo, and Paul A. Haynes. 2023. "Proteome-Wide Profiling Using Sample Multiplexing of a Human Cell Line Treated with Cannabidiol (CBD) and Tetrahydrocannabinol (THC)" Proteomes 11, no. 4: 36. https://doi.org/10.3390/proteomes11040036

APA Style

Abyadeh, M., Gupta, V., Liu, X., Rossio, V., Mirzaei, M., Cornish, J., Paulo, J. A., & Haynes, P. A. (2023). Proteome-Wide Profiling Using Sample Multiplexing of a Human Cell Line Treated with Cannabidiol (CBD) and Tetrahydrocannabinol (THC). Proteomes, 11(4), 36. https://doi.org/10.3390/proteomes11040036

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop