The Chemical and Biological Profiles of Leaves from Commercial Blueberry Varieties
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials
2.2. Chemicals
2.3. Biomolecules Extraction Assisted by Ultrasounds
2.4. Evaluation of Phenolic Profile and Content
2.4.1. HPLC-DAD-ESI-MS Chemical Profile
2.4.2. The Total Content of Phenolics
2.4.3. The Total Content of Flavonoids
2.4.4. The Total Content of Anthocyanins
2.5. DPPH Antioxidant Capacity
2.6. Antimicrobial Activity
2.6.1. Microorganisms
2.6.2. Microdilution Technique
2.6.3. Antifungal Test
2.7. Antimutagenic Activity
2.8. Statistical Interpretations of Results
3. Results and Discussion
3.1. Phenolic Profile of the Leaf Extracts from the Six Blueberry Varieties
3.2. Total Phenolic and Total Flavonoid Contents
3.3. Total Anthocyanins Content
3.4. DPPH Radical Scavenging Activity
3.5. Evaluation of the Antimicrobial Activity
3.6. Evaluation of the Antimutagenic Capacity of Romanian Blueberry Cultivars
4. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Peak No. | Retention Time Rt (min) | UV λmax (nm) | [M + H] + (m/z) | Compound | Subclass |
---|---|---|---|---|---|
1 | 2.97 | 279 | 307, 290 | Gallocatechin | Flavanol |
2 | 11.33 | 280 | 579, 291 | Procyanidin dimer I | Flavanol |
3 | 12.01 | 281, 329 | 355, 163 | 5-Caffeoylquinic acid (Chlorogenic acid) | Hydroxycinnamic acid |
4 | 12.58 | 280 | 291 | Catechin | Flavanol |
5 | 13.11 | 280 | 291 | Epicatechin | Flavanol |
6 | 13.41 | 282, 329 | 181, 163 | Caffeic acid | Hydroxycinnamic acid |
7 | 13.89 | 280 | 865, 291 | Procyanidin trimer | Flavanol |
8 | 14.79 | 283, 330 | 369 | Feruloylquinic acid I | Hydroxycinnamic acid |
9 | 15.35 | 263, 355 | 611, 303 | Quercetin-rutinoside (Rutin) | Flavonol |
10 | 16.20 | 263, 355 | 465, 303 | Quercetin-glucoside | Flavonol |
11 | 17.83 | 263, 356 | 493, 303 | Quercetin-acetyl-rhamnoside | Flavonol |
12 | 18.69 | 262, 355 | 435, 303 | Quercetin-arabinoside | Flavonol |
13 | 19.74 | 280 | 579, 291 | Procyanidin dimer II | Flavanol |
14 | 20.08 | 282, 329 | 517, 163 | Dicaffeoylquinic acid | Hydroxycinnamic acid |
15 | 21.15 | 263, 355 | 628, 303 | Quercetin-diglucoside | Flavonol |
16 | 21.88 | 261, 355 | 303 | Quercetin | Flavonol |
17 | 11.02 | 210, 517 | 449, 287 | Cyanidin-glucoside | Anthocyanins |
18 | 11.78 | 214, 517 | 419, 287 | Cyanidin-arabinoside | Anthocyanins |
19 | 14.28 | 218, 518 | 491, 287 | Cyanidin-acetyl-glucoside | Anthocyanins |
Phenolic Compounds | Cultivars | ||||||
---|---|---|---|---|---|---|---|
Elliot | Toro | Duke | Bluecrop | Spartan | Nelson | ||
Flavanols | Gallocatechin | 8.52 ± 0.07 b | 9.03 ± 0.08 a | 4.84 ± 0.05 e | 4.90 ± 0.05 e | 7.18 ± 0.06 c | 6.73 ± 0.08 d |
Catechin | 6.46 ± 0.07 b | 7.97 ± 0.07 a | 6.03 ± 0.07 c | 5.21 ± 0.05 d | 6.15 ± 0.05 c | 4.87 ± 0.08 e | |
Epicatechin | 4.19 ± 0.03 | n.d | n.d | n.d | n.d | n.d | |
Procyanidin dimer I | 3.69 ± 0.04 | n.d | n.d | n.d | n.d | n.d | |
Procyanidin dimer II | 12.50 ± 0.1 c | 14.13 ± 0.1 b | 12.30 ± 0.1 d | 12.41 ± 0.1 cd | 17.15 ± 0.1 a | 3.92 ± 0.03 e | |
Procyanidin trimer | 6.36 ± 0.08 f | 15.99 ± 0.2 a | 7.02 ± 0.12 d | 8.98 ± 0.10 c | 6.80 ± 0.09 de | 12.27 ± 0.1 b | |
Hydroxycinnamic acids | Chlorogenic acid | 0.90 ± 0.01 c | 1.23 ± 0.02 a | 0.52 ± 0.01 d | 0.54 ± 0.01 d | 0.44 ± 0.01 e | 1.03 ± 0.01 b |
Caffeic acid | 5.93 ± 0.06 a | 4.75 ± 0.03 c | 4.49 ± 0.05 d | 2.62 ± 0.02 f | 3.61 ± 0.02 e | 5.36 ± 0.05 b | |
Feruloylquinic acid | 40.94 ± 0.3 c | 44.43 ± 0.3 b | 29.15 ± 0.2 d | 25.36 ± 0.2 e | 19.23 ± 0.1 f | 49.62 ± 0.4 a | |
Dicaffeoylquinic acid | 7.99 ± 0.07 b | 7.30 ± 0.09 c | 4.23 ± 0.05 d | 2.73 ± 0.02 e | 4.58 ± 0.03 d | 10.18 ± 0.1 a | |
Flavonols (quercetin derivatives) | Quercetin-rutinoside (Rutin) | 31.53 ± 0.1 c | 35.77 ± 0.1 a | 17.64 ± 0.1 e | 19.10 ± 0.1 d | 14.44 ± 0.1 f | 32.50 ± 0.2 b |
Quercetin-glucoside | 3.46 ± 0.04 b | 6.70 ± 0.08 a | 2.86 ± 0.02 d | 3.54 ± 0.03 b | 3.08 ± 0.03 c | 2.09 ± 0.02 e | |
Quercetin-acetyl-rhamnoside | 1.22 ± 0.01 b | 1.92 ± 0.02 a | 0.45 ± 0.01 e | 0.68 ± 0.01 d | 0.46 ± 0.01 e | 0.78 ± 0.01 c | |
Quercetin-arabinoside | n.d | n.d | n.d | n.d | 1.40 ± 0.01 | n.d | |
Quercetin-diglucoside | 0.35 ± 0.01 b | 0.53 ± 0.01 a | n.d | n.d | n.d | n.d | |
Quercetin | 2.70 ± 0.02 c | 3.68 ± 0.04 a | 2.25 ± 0.06 d | 2.07 ± 0.04 e | 3.69 ± 0.02 a | 3.56 ± 0.02 ab | |
Anthocyanins | Cyanidin-glucoside | 0.63 ± 0.01 b | 1.08 ± 0.08 a | n.d | n.d | 0.52 ± 0.01 b | n.d |
Cyanidin-arabinoside | 0.41 ± 0.01 b | 0.80 ± 0.01 a | n.d | n.d | 0.42 ± 0.01 b | n.d | |
Cyanidin-acetyl-glucoside | 0.32 ± 0.01 a | 0.35 ± 0.01 a | n.d | n.d | 0.26 ± 0.01 b | n.d | |
Total phenols | 138.09 b | 155.67 a | 92.46 d | 88.14 e | 89.40 e | 132.91 c |
Type of Strains | Gram-Positive | Gram-Negative | Fungi | |||||||
---|---|---|---|---|---|---|---|---|---|---|
Sample | Varieties | S. aureus | E. fecalis | R. equi | E. coli enterotoxigen | K. pneumonia | P. aeruginosa | Candida albicans | Candida zeylanoides | Candida parapsilosis |
mg/mL | ||||||||||
Leaves | Elliot | 0.12 | 0.12 | 0.06 | 0.48 | 0.12 | 0.48 | 125 | 62.5 | 31.25 |
Toro | 0.06 | 0.12 | 0.06 | 0.48 | 0.12 | 1.92 | 125 | 62.5 | 31.25 | |
Duke | 0.24 | 0.24 | 0.06 | 0.48 | 0.12 | 0.96 | 125 | 62.5 | 31.25 | |
Bluecrop | 0.12 | 0.24 | 0.06 | 0.48 | 0.24 | 0.48 | 125 | 31.25 | 31.25 | |
Spartan | 0.06 | 0.12 | 0.06 | 0.48 | 0.12 | 1.92 | 125 | 62.5 | 31.25 | |
Nelson | 0.12 | 0.12 | 0.06 | 0.48 | 0.12 | 0.48 | 125 | 62.5 | 31.25 | |
Control | Fluconazole μg/mL | - | - | - | - | - | - | 15.62 | 7.81 | 15.62 |
Streptomicyn μg/mL | 0.03 | 0.06 | 0.06 | 0.12 | 0.06 | 0.06 | - | - | - |
Type of Strains | Gram-Positive | Gram-Negative | Fungi | |||||||
---|---|---|---|---|---|---|---|---|---|---|
Sample | Varieties | S. aureus | E. fecalis | R. equi | E. coli enterotoxigen | K. pneumonia | P. aeruginosa | Candida albicans | Candida zeylanoides | Candida parapsilosis |
mg/mL | ||||||||||
Leaves | Elliot | 0.24 | 0.24 | 0.12 | 0.96 | 0.24 | 0.96 | 250 | 125 | 62.5 |
Toro | 0.12 | 0.24 | 0.12 | 0.96 | 0.24 | 3.84 | 250 | 125 | 62.5 | |
Duke | 0.48 | 0.48 | 0.12 | 0.96 | 0.24 | 1.92 | 250 | 125 | 62.5 | |
Bluecrop | 0.24 | 0.48 | 0.12 | 0.96 | 0.48 | 0.96 | 250 | 62.5 | 62.5 | |
Spartan | 0.12 | 0.24 | 0.12 | 0.96 | 0.24 | 3.84 | 250 | 125 | 62.5 | |
Nelson | 0.24 | 0.24 | 0.12 | 0.96 | 0.24 | 0.96 | 250 | 125 | 62.5 | |
Control | Fluconazole μg/mL | - | - | - | - | - | - | 31.24 | 15.62 | 31.24 |
Streptomicyn μg/mL | 0.06 | 0.12 | 0.12 | 0.24 | 0.12 | 0.12 | - | - | - |
Sample | Varieties | Number of Revertants | |||
---|---|---|---|---|---|
TA98 | TA100 | ||||
Mean ± S.D | Inhibition % | Mean ± S.D | Inhibition % | ||
Leaves | Negative control | 9.35 ± 3.2 a | 9.35 ± 2.1 a | ||
Elliot | 157 ± 5.4 | 19.07 | 250 ± 6.5 | 28.36 | |
Toro | 130 ± 5.9 | 32.98 | 214 ± 3.2 | 38.68 | |
Duke | 152 ± 7.8 | 21.64 | 221 ± 6.5 | 36.67 | |
Bluecrop | 142 ± 8.6 | 26.8 | 224 ± 4.4 | 35.81 | |
Spartan | 139 ± 5.7 | 28.35 | 232 ± 4.1 | 33.52 | |
Nelson | 151 ± 6.2 | 22.16 | 254 ± 4.4 | 27.22 | |
4-NPD b | 194 ± 3.3 | - | - | - | |
NaN3 b | - | - | 349 ± 15.22 | - |
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Ștefănescu, B.-E.; Călinoiu, L.F.; Ranga, F.; Fetea, F.; Mocan, A.; Vodnar, D.C.; Crișan, G. The Chemical and Biological Profiles of Leaves from Commercial Blueberry Varieties. Plants 2020, 9, 1193. https://doi.org/10.3390/plants9091193
Ștefănescu B-E, Călinoiu LF, Ranga F, Fetea F, Mocan A, Vodnar DC, Crișan G. The Chemical and Biological Profiles of Leaves from Commercial Blueberry Varieties. Plants. 2020; 9(9):1193. https://doi.org/10.3390/plants9091193
Chicago/Turabian StyleȘtefănescu, Bianca-Eugenia, Lavinia Florina Călinoiu, Floricuța Ranga, Florinela Fetea, Andrei Mocan, Dan Cristian Vodnar, and Gianina Crișan. 2020. "The Chemical and Biological Profiles of Leaves from Commercial Blueberry Varieties" Plants 9, no. 9: 1193. https://doi.org/10.3390/plants9091193