Overexpression of Sucrose Phosphate Synthase Enhanced Sucrose Content and Biomass Production in Transgenic Sugarcane
Abstract
:1. Introduction
2. Results
2.1. Expression of SoSPS1 Gene in Transgenic Sugarcane
2.2. Sucrose Metabolizing Enzymes Activities
2.3. Increasing Sugar Content in the Leaves and Stalks of Transgenic Sugarcane
2.4. The Effect of SPS Overexpression on Sugarcane Growth
3. Discussion
4. Materials and Methods
4.1. Plant Transformation and Growth Condition
4.2. Genomic and Gene Expression Analysis
4.3. Protein Extraction, Enzyme Assay, and Immunoblotting
4.4. Sugar Analysis
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Lines | Leaf Tissue | Stalk Tissue | ||||
---|---|---|---|---|---|---|
Sucrose (mg/g FW) | Fructose (mg/g FW) | Glucose (mg/g FW) | Sucrose (mg/g FW) | Fructose (mg/g FW) | Glucose (mg/g FW) | |
NT | 2.27 ± 0.10 c | 0.35 ± 0.17 b | 0.18 ± 0.06 b | 71.07 ± 3.30 b | 2.33 ± 0.31 b | 3.23 ± 1.02 c |
SP1 | 3.59 ± 0.04 b | 3.34 ± 0.39 a | 2.21 ± 0.58 a | 80.40 ± 8.32 b | 2.87 ± 0.46 ab | 4.40 ± 1.15 bc |
SP3 | 5.51 ± 0.24 a | 3.38 ± 0.58 a | 1.52 ± 0.64 ab | 94.23 ± 3.34 a | 3.62 ± 0.20 a | 6.25 ± 1.06 a |
SP9 | 3.02 ± 0.34 b | 2.47 ± 0.08 a | 1.30 ± 0.83 ab | 98.52 ± 5.55 a | 3.31 ± 0.26 ab | 4.86 ± 0.30 ab |
Lines | Plant Height (cm) | Stalk Diameter (cm) | Stalk Number | Stalk Weight per Pot (g) |
---|---|---|---|---|
NT | 99.67 ± 3.67 b | 2.24 ± 0.03 b | 9.00 ± 1.00 b | 3537.90 ± 680 b |
SP1 | 115.33 ± 6.00 a | 2.38 ± 0.03 a | 10.67 ± 1.53 ab | 4193.06 ± 600 ab |
SP3 | 112.78 ± 5.01 a | 2.24 ± 0.04 b | 12.67 ± 0.58 a | 4979.26 ± 226 a |
SP9 | 124.33 ± 3.93 a | 2.18 ± 0.05 b | 11.67 ± 0.58 a | 4586.16 ± 226 ab |
Primer Names | Sequence (5′–3′) | Product (bp) | Target Genes |
---|---|---|---|
F1 | TGAATGAACTGCAGGACGAG | 550 | npt II |
R1 | AGCCAACGTATGTCCTGAT | 550 | npt II |
F2 | TGAAGGACACACCGGCAGATG | 750 | SoSPS1 |
R2 | CTTTGATGAGGAAGGCGAAGC | 750 | SoSPS1 |
F3 | GCAACTGGGATGACATGGAG | 568 | Actin |
R3 | ATGGCTGGAAGAGGACCTCAG | 568 | Actin |
F4 | TGCACTAGTCGCCCTTCCCA | 3425 | SoSPS1 |
R4 | TCCACTAGTAACGGCCGCCA | 3425 | SoSPS1 |
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Anur, R.M.; Mufithah, N.; Sawitri, W.D.; Sakakibara, H.; Sugiharto, B. Overexpression of Sucrose Phosphate Synthase Enhanced Sucrose Content and Biomass Production in Transgenic Sugarcane. Plants 2020, 9, 200. https://doi.org/10.3390/plants9020200
Anur RM, Mufithah N, Sawitri WD, Sakakibara H, Sugiharto B. Overexpression of Sucrose Phosphate Synthase Enhanced Sucrose Content and Biomass Production in Transgenic Sugarcane. Plants. 2020; 9(2):200. https://doi.org/10.3390/plants9020200
Chicago/Turabian StyleAnur, Risky Mulana, Nurul Mufithah, Widhi Dyah Sawitri, Hitoshi Sakakibara, and Bambang Sugiharto. 2020. "Overexpression of Sucrose Phosphate Synthase Enhanced Sucrose Content and Biomass Production in Transgenic Sugarcane" Plants 9, no. 2: 200. https://doi.org/10.3390/plants9020200