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Article

Overexpression of Sucrose Phosphate Synthase Enhanced Sucrose Content and Biomass Production in Transgenic Sugarcane

1
Center for Development of Advanced Science and Technology (CDAST), University of Jember, Jember 68121, Indonesia
2
Present address: Department of Agronomy, Faculty of Agriculture, University of Gadjahmada, Yogyakarta 55281, Indonesia
3
RIKEN Center for Sustainable Resource Sciences, Yokohama 230-0045, Japan
4
Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan
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Department of Biology, Faculty of Mathematic and Natural Science, University of Jember, Jember 68121, Indonesia
*
Author to whom correspondence should be addressed.
Plants 2020, 9(2), 200; https://doi.org/10.3390/plants9020200
Received: 13 December 2019 / Revised: 4 February 2020 / Accepted: 5 February 2020 / Published: 6 February 2020
Sucrose phosphate synthase (SPS) is a key enzyme in sucrose synthesis, which controls sucrose content in plants. This study was designed to examine the efficacy of the overexpression of SoSPS1 gene on sucrose accumulation and carbon partitioning in transgenic sugarcane. The overexpression of SoSPS1 gene increased SPS activity and sucrose content in transgenic sugarcane leaves. More importantly, the overexpression enhanced soluble acid invertase (SAI) activity concomitant with the increase of glucose and fructose levels in the leaves, whereas sucrose synthase activity exhibited almost no change. In the stalk, a similar correlation was observed, but a higher correlation was noted between SPS activity and sugar content. These results suggest that SPS overexpression has both direct and indirect effects on sugar concentration and SAI activity in sugarcane. In addition, SPS overexpression resulted in a significant increase in plant height and stalk number in some transgenic lines compared to those in non-transgenic control. Taken together, these results strongly suggest that enhancing SPS activity is a useful strategy for improving sugarcane yield. View Full-Text
Keywords: biomass; sucrose; soluble acid invertase; sucrose phosphate synthase; transgenic sugarcane biomass; sucrose; soluble acid invertase; sucrose phosphate synthase; transgenic sugarcane
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MDPI and ACS Style

Anur, R.M.; Mufithah, N.; Sawitri, W.D.; Sakakibara, H.; Sugiharto, B. Overexpression of Sucrose Phosphate Synthase Enhanced Sucrose Content and Biomass Production in Transgenic Sugarcane. Plants 2020, 9, 200. https://doi.org/10.3390/plants9020200

AMA Style

Anur RM, Mufithah N, Sawitri WD, Sakakibara H, Sugiharto B. Overexpression of Sucrose Phosphate Synthase Enhanced Sucrose Content and Biomass Production in Transgenic Sugarcane. Plants. 2020; 9(2):200. https://doi.org/10.3390/plants9020200

Chicago/Turabian Style

Anur, Risky M., Nurul Mufithah, Widhi D. Sawitri, Hitoshi Sakakibara, and Bambang Sugiharto. 2020. "Overexpression of Sucrose Phosphate Synthase Enhanced Sucrose Content and Biomass Production in Transgenic Sugarcane" Plants 9, no. 2: 200. https://doi.org/10.3390/plants9020200

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