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Article

Spider Silk Fibroin Protein Heterologously Produced in Rice Seeds Reduce Diabetes and Hypercholesterolemia in Mice

1
College of Life Science and Natural Resources, Dong-A University, Busan 49315, Korea
2
College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
3
National Key Laboratory of Crop Genetic Improvement, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to the work.
Plants 2020, 9(10), 1282; https://doi.org/10.3390/plants9101282
Received: 6 August 2020 / Revised: 25 September 2020 / Accepted: 26 September 2020 / Published: 28 September 2020
(This article belongs to the Special Issue Plant Molecular Breeding and Biotechnology)
Silk fibroin proteins are biomaterials with diverse applications. These spider and silkworm proteins have specific biological effects when consumed by mammals; in addition to reducing blood pressure and blood glucose and cholesterol levels, they have anti-human immunodeficiency virus activity. In the present study, rice (Oryza sativa) was engineered to produce the C-terminus of the major ampullate spidroin protein from the spider Araneus ventricosus under the control of a Prolamin promoter. Homozygous transgenic rice lines were identified, and the therapeutic effect of this spider silk fibroin protein on the lipid and glucose metabolism was analyzed in a mouse model. Feeding fat-fed mice, the transgenic rice seeds for four weeks reduced serum concentrations of triglycerides, total cholesterol, low-density lipoprotein cholesterol, glutamic oxaloacetic transaminase, and glutamic pyruvic transaminase, and lowered blood glucose levels. This is the first study to investigate the effects of consumption of rice seeds heterologously expressing spider silk fibroin protein in a mammalian model. Our findings suggest that functional foods containing spider silk fibroin protein might be useful as potential pharmaceutical materials for preventing and treating diabetes, hyperlipidemia, and hypercholesterolemia. View Full-Text
Keywords: transgenic rice; spider silk fibroin protein; glucose metabolism; lipid metabolism transgenic rice; spider silk fibroin protein; glucose metabolism; lipid metabolism
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MDPI and ACS Style

Yang, W.T.; Lee, K.S.; Hur, Y.J.; Kim, B.Y.; Li, J.; Yu, S.; Jin, B.R.; Kim, D.H. Spider Silk Fibroin Protein Heterologously Produced in Rice Seeds Reduce Diabetes and Hypercholesterolemia in Mice. Plants 2020, 9, 1282. https://doi.org/10.3390/plants9101282

AMA Style

Yang WT, Lee KS, Hur YJ, Kim BY, Li J, Yu S, Jin BR, Kim DH. Spider Silk Fibroin Protein Heterologously Produced in Rice Seeds Reduce Diabetes and Hypercholesterolemia in Mice. Plants. 2020; 9(10):1282. https://doi.org/10.3390/plants9101282

Chicago/Turabian Style

Yang, Won T., Kwang S. Lee, Yeon J. Hur, Bo Y. Kim, Jianhong Li, Sibin Yu, Byung R. Jin, and Doh H. Kim. 2020. "Spider Silk Fibroin Protein Heterologously Produced in Rice Seeds Reduce Diabetes and Hypercholesterolemia in Mice" Plants 9, no. 10: 1282. https://doi.org/10.3390/plants9101282

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