Next Article in Journal
The Morphological Features and Biology of a Relict and Endangered Woody Plant Species: Chamaedaphne calyculata (L.) Moench (Ericaceae)
Next Article in Special Issue
Expanding Avenue of Fast Neutron Mediated Mutagenesis for Crop Improvement
Previous Article in Journal
Non-cultivated Cotton Species (Gossypium spp.) Act as a Reservoir for Cotton Leaf Curl Begomoviruses and Associated Satellites
Article Menu
Issue 5 (May) cover image

Export Article

Open AccessReview

Mutation Breeding in Tomato: Advances, Applicability and Challenges

Department of Biology, Oberlin College, Oberlin, OH 44074, USA
National Agri-Food Biotechnology Institute (NABI), Mohali, Punjab 140308, India
National Research Center on Plant Biotechnology, New Delhi, Delhi 110012, India
Division of Plant Pathology, ICAR-IARI, New Delhi, Delhi 110001, Inida
National Bureau of Plant Genetic Resources, New Delhi, Delhi 110012, India
Faculté des sciences de l’agriculture et de l’alimentation (FSAA), Université Laval, Quebec, QC G1V 0A6, Canada
Authors to whom correspondence should be addressed.
Plants 2019, 8(5), 128;
Received: 1 April 2019 / Revised: 9 May 2019 / Accepted: 11 May 2019 / Published: 14 May 2019
(This article belongs to the Special Issue Advances in Plant Mutagenesis Research)
PDF [891 KB, uploaded 14 May 2019]


Induced mutagenesis is one of the most effective strategies for trait improvement without altering the well-optimized genetic background of the cultivars. In this review, several currently accessible methods such as physical, chemical and insertional mutagenesis have been discussed concerning their efficient exploration for the tomato crop improvement. Similarly, challenges for the adaptation of genome-editing, a newly developed technique providing an opportunity to induce precise mutation, have been addressed. Several efforts of genome-editing have been demonstrated in tomato and other crops, exploring its effectiveness and convenience for crop improvement. Descriptive data compiled here from such efforts will be helpful for the efficient exploration of technological advances. However, uncertainty about the regulation of genome-edited crops is still a significant concern, particularly when timely trait improvement in tomato cultivars is needed. In this regard, random approaches of induced mutagenesis are still promising if efficiently explored in breeding applications. Precise identification of casual mutation is a prerequisite for the molecular understanding of the trait development as well as its utilization for the breeding program. Recent advances in sequencing techniques provide an opportunity for the precise detection of mutagenesis-induced sequence variations at a large scale in the genome. Here, we reviewed several novel next-generation sequencing based mutation mapping approaches including Mutmap, MutChromeSeq, and whole-genome sequencing-based mapping which has enormous potential to accelerate the mutation breeding in tomato. The proper utilization of the existing well-characterized tomato mutant resources combined with novel mapping approaches would inevitably lead to rapid enhancement of tomato quality and yield. This article provides an overview of the principles and applications of mutagenesis approaches in tomato and discusses the current progress and challenges involved in tomato mutagenesis research. View Full-Text
Keywords: genome-editing; mutagenesis approaches; mutmap; mutation breeding; next generation sequencing tools; tomato genome-editing; mutagenesis approaches; mutmap; mutation breeding; next generation sequencing tools; tomato

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Share & Cite This Article

MDPI and ACS Style

Chaudhary, J.; Alisha, A.; Bhatt, V.; Chandanshive, S.; Kumar, N.; Mir, Z.; Kumar, A.; Yadav, S.K.; Shivaraj, S.M.; Sonah, H.; Deshmukh, R. Mutation Breeding in Tomato: Advances, Applicability and Challenges. Plants 2019, 8, 128.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Plants EISSN 2223-7747 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top