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A Polyamine Oxidase from Selaginella lepidophylla (SelPAO5) can Replace AtPAO5 in Arabidopsis through Converting Thermospermine to Norspermidine instead to Spermidine

1
Department of Genetics and Plant Breeding, Faculty of Agriculture, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh
2
Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Aoba, Sendai 980-8577, Japan
3
Laboratory Center, Senckenberg Biodiversity and Climate Research Center, Georg-Voigt-Str. 14-16, D-60325 Frankfurt am Main, Germany
*
Author to whom correspondence should be addressed.
Plants 2019, 8(4), 99; https://doi.org/10.3390/plants8040099
Received: 11 February 2019 / Revised: 8 April 2019 / Accepted: 11 April 2019 / Published: 15 April 2019
(This article belongs to the Special Issue Plant Polyamines)
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Abstract

Of the five polyamine oxidases in Arabidopsis thaliana, AtPAO5 has a substrate preference for the tetraamine thermospermine (T-Spm) which is converted to triamine spermidine (Spd) in a back-conversion reaction in vitro. A homologue of AtPAO5 from the lycophyte Selaginella lepidophylla (SelPAO5) back-converts T-Spm to the uncommon polyamine norspermidine (NorSpd) instead of Spd. An Atpao5 loss-of-function mutant shows a strong reduced growth phenotype when growing on a T-Spm containing medium. When SelPAO5 was expressed in the Atpao5 mutant, T-Spm level decreased to almost normal values of wild type plants, and NorSpd was produced. Furthermore the reduced growth phenotype was cured by the expression of SelPAO5. Thus, a NorSpd synthesis pathway by PAO reaction and T-Spm as substrate was demonstrated in planta and the assumption that a balanced T-Spm homeostasis is needed for normal growth was strengthened. View Full-Text
Keywords: polyamine oxidase; norspermidine; thermospermine; Selaginella lepidophylla; Arabidopsis thaliana mutant polyamine oxidase; norspermidine; thermospermine; Selaginella lepidophylla; Arabidopsis thaliana mutant
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Sagor, G.H.M.; Kusano, T.; Berberich, T. A Polyamine Oxidase from Selaginella lepidophylla (SelPAO5) can Replace AtPAO5 in Arabidopsis through Converting Thermospermine to Norspermidine instead to Spermidine. Plants 2019, 8, 99.

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