Phenolic Composition and Antimicrobial Activity of Different Emirati Date (Phoenix dactylifera L.) Pits: A Comparative Study
Abstract
:1. Introduction
2. Materials and Methods
2.1. Date Pits Material
2.2. Date Fruit De-Pitting and Collection of Pits
2.3. Organic Solvent Extraction and Treatment Solution Preparation
2.4. Proximate Analysis
2.5. Dietary Fiber Analysis
2.6. Total Phenolic Content (Folin–Ciocalteau Assay)
2.7. Total Flavonoids
2.8. In Vitro Anti-Microbial Bioassays
2.9. Minimum Inhibitory Concentration
2.10. Statistical Analysis
3. Results
3.1. Proximate Analysis
3.2. Dietary Fiber Composition
3.3. Phenolic Compounds and Flavonoids
3.4. Experiment I
3.5. Experiment II
3.6. Minimum Inhibitory Concentration
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Date Palm Variety | Moisture | Fats | Protein | Ash | Carbohydrates | Dietary Fiber |
---|---|---|---|---|---|---|
Khalas | 16.28 ± 0.25 a | 10.24 ± 0.04 b | 5.21 ± 0.06 c | 0.15 ± 0.12 b | 4.80 ± 0.06 a | 72.74 ± 0.10 c |
Lulu | 11.14 ± 0.06 c | 8.45 ± 0.22 c | 6.25 ± 0.10 b | 1.45 ± 0.05 a | 3.20 ± 0.14 b | 70.10 ± 0.20 d |
Fard | 9.72 ± 0.20 e | 10.72 ± 0.10 b | 5.74 ± 0.14 c | 0.50 ± 0.22 b | 3.40 ± 0.10 b | 70.25 ± 0.24 d |
Ajwa | 10.6 ± 0.04 d | 10.45 ± 0.06 b | 8.62 ± 0.06 a | 0.74 ± 0.10 b | 2.80 ± 0.10 c | 74.60 ± 0.18 b |
Abu Maan | 10.32 ± 0.14 d | 8.25 ± 0.04 c | 5.67 ± 0.14 c | 1.80 ± 0.26 a | 3.20 ± 0.20 b | 75.50 ± 0.13 a |
Mabroom | 12.44 ± 0.10 b | 12.57 ± 0.10 a | 6.2 ± 0.20 b | 1.24 ± 0.04 a | 2.10 ± 0.22 c | 68.04 ± 0.15 e |
Date Palm Variety | Total Phenolic Content (mg GAE/100 g dw) | Total Flavonoid Contents (mg CEQ/100 g dw) |
---|---|---|
Khalas | 7.28 ± 0.02 a | 4.30 ± 0.02 a |
Lulu | 6.40 ± 0.03 b | 2.70 ± 0.04 c |
Fard | 6.10 ± 0.020 b | 1.60 ± 0.01 d |
Ajwa | 7.80 ± 0.05 a | 4.54 ± 0.0 a |
Abu Maan | 5.00 ± 0.01 c | 3.80 ± 0.10 b |
Mabroom | 4.65 ± 0.04 c | 3.50 ± 0.04 b |
# | Extract | Inhibition Zone Diameter (mm) | ||
---|---|---|---|---|
Staphylococcus aureus (ATCC29213) | Escherichia coli (ATCC 25922) | Candida albicans (ATCC 66027) | ||
1 | Khalas (Ethyl acetate) | 20 ± 1.2 a | 15 ± 1.1 | 0 |
2 | Khalas (Hexane) | 0 | 0 | 0 |
3 | Khalas (H2O: EtOH (1:1) | 0 | 0 | 0 |
4 | Khodari (Ethyl acetate) | 20 ± 1.3 a | 0 | 0 |
5 | Khodari (Hexane) | 0 | 0 | 0 |
6 | Khodari (H2O: EtOH (1:1) | 0 | 0 | 0 |
# | Extract | Inhibition Zone Diameter (mm) | ||
---|---|---|---|---|
Staphylococcus aureus (ATCC29213) | Escherichia coli (ATCC 25922) | Candida albicans (ATCC 66027) | ||
1 | Khalas (ethyl acetate) | 20 ± 1.0 b | 0 | 0 |
2 | Khalas (hexane) | 0 | 7 ± 0.45 b | 0 |
3 | Khalas (H2O: EtOH (1:1) | 12 ± 1.0 d | 7 ± 0.6 b | 0 |
4 | Khodari (ethyl acetate) | 22 ± 1.5 a | 11 ± 0.9 a | 0 |
5 | Khodari (hexane) | 17 ± 1.2 c | 12 ± 1.0 a | 0 |
6 | Khodari (H2O: EtOH (1:1) | 0 | 0 | 0 |
7 | Amikacin (30 μg) | 22 | 25 | - |
8 | Fluconazole (25 μg) | - | - | 29 |
Bacteria | Treatment | Diameter of Inhibition Zone (mm) | ||||||
---|---|---|---|---|---|---|---|---|
Khalas | Abu Maan | Ajwa | Fard | Lulu | Mabroom | |||
Gram-positive | Acet + H2O | 13 ± 0.3 a | 15 ± 0.3 a | 13 ± 0.2 a | 6 ± 0.1 b | 10 ± 0.2 a | 6 ± 0.1 b | |
S. aureus | ETAC | 8 ± 0.2 c | 8 ± 0.19 b | 11 ± 0.19 b | 6 ± 0.1 b | 6 ± 0.1 b | 6 ± 0.1 b | |
(ATCC29213) | MeOH + CFM | 9 ± 0.2 b | 15 ± 0.4 a | 10 ± 0.2 c | 10 ± 0.2 a | 6 ± 0.1 b | 13 ± 0.3 a | |
Gram-negative | Acet + H2O | 13 ± 0.3 a | 12 ± 0.3 b | 15 ± 0.4 c | 6 ± 0.19 a | 12 ± 0.3 a | 6 ± 0.1 a | |
E. coli | ETAC | 12 ± 0.3 b | 12 ± 0.19 b | 16 ± 0.38 b | 6 ± 0.18 a | 10 ± 0.19 b | 6 ± 0.14 a | |
(ATCC 25922) | MeOH + CFM | 10 ± 0.2 c | 15 ± 0.34 a | 18 ± 0.5 a | 6 ± 0.2 a | 6 ± 0.17 c | 6 ± 0.12 a | |
Fungi | Acet + H2O | 0 | 0 | 0 | 0 | 0 | 0 | |
C. albicans | ETAC | 0 | 0 | 0 | 0 | 0 | 0 | |
(ATCC 66027) | MeOH + CFM | 0 | 0 | 0 | 0 | 0 | 0 |
Code | Date Palm Variety | Organic Solvents | Date Pit Extract (mg/mL) |
---|---|---|---|
(MIC) | |||
A1 | Khalas | Acetone + water (1:1) | 10 ± 0.3 |
B1 | Abu Maan | Acetone + water (1:1) | 10 ± 0.3 |
B3 | Abu Maan | MeOH + chloroform (1:1) | 2.5 ± 0.3 |
C1 | Ajwa | Acetone + water (1:1) | 7.5 ± 0.5 |
C3 | Ajwa | MeOH + chloroform (1:1) | 5 ± 0.3 |
D3 | Fard | MeOH + chloroform (1:1) | 7.5 ± 0.3 |
E1 | Lulu | Acetone + water (1:1) | 5 ± 0.5 |
E2 | Lulu | Ethyl acetate | 7.5 ± 0.3 |
F1 | Mabroom | Acetone + water (1:1) | 2.5 ± 0.5 |
F3 | Mabroom | MeOH + chloroform (1:1) | 5 ± 0.3 |
Positive Control (Vancomycin) | 10 µg/mL |
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Hussain, M.I.; Semreen, M.H.; Shanableh, A.; Khattak, M.N.K.; Saadoun, I.; Ahmady, I.M.; Mousa, M.; Darwish, N.; Radeef, W.; Soliman, S.S.M. Phenolic Composition and Antimicrobial Activity of Different Emirati Date (Phoenix dactylifera L.) Pits: A Comparative Study. Plants 2019, 8, 497. https://doi.org/10.3390/plants8110497
Hussain MI, Semreen MH, Shanableh A, Khattak MNK, Saadoun I, Ahmady IM, Mousa M, Darwish N, Radeef W, Soliman SSM. Phenolic Composition and Antimicrobial Activity of Different Emirati Date (Phoenix dactylifera L.) Pits: A Comparative Study. Plants. 2019; 8(11):497. https://doi.org/10.3390/plants8110497
Chicago/Turabian StyleHussain, M. Iftikhar, Mohammad H. Semreen, Abdallah Shanableh, Muhammad Nasir Khan Khattak, Ismail Saadoun, Islam M. Ahmady, Muath Mousa, Nora Darwish, Wameed Radeef, and Sameh S. M. Soliman. 2019. "Phenolic Composition and Antimicrobial Activity of Different Emirati Date (Phoenix dactylifera L.) Pits: A Comparative Study" Plants 8, no. 11: 497. https://doi.org/10.3390/plants8110497
APA StyleHussain, M. I., Semreen, M. H., Shanableh, A., Khattak, M. N. K., Saadoun, I., Ahmady, I. M., Mousa, M., Darwish, N., Radeef, W., & Soliman, S. S. M. (2019). Phenolic Composition and Antimicrobial Activity of Different Emirati Date (Phoenix dactylifera L.) Pits: A Comparative Study. Plants, 8(11), 497. https://doi.org/10.3390/plants8110497