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Plants 2017, 6(4), 53; https://doi.org/10.3390/plants6040053

Genus-Specific Real-Time PCR and HRM Assays to Distinguish Liriope from Ophiopogon Samples

1
Biomolecular Technology Group, Allied Health Sciences, Faculty of Health and Life Sciences, De Montfort University, Leicester LE1 9BH, UK
2
CSIR-North East Institute of Science & Technology, Jorhat 785006, Assam, India
3
Environmental Industries and Business, Brooksby Melton College, Brooksby Campus, Brooksby, Leicestershire LE14 2LJ, UK
*
Author to whom correspondence should be addressed.
Received: 20 September 2017 / Revised: 17 October 2017 / Accepted: 19 October 2017 / Published: 26 October 2017
(This article belongs to the Special Issue Medicinal Plants and Natural Product Research)
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Abstract

Liriope and Ophiopogon species have a long history of use as traditional medicines across East Asia. They have also become widely used around the world for ornamental and landscaping purposes. The morphological similarities between Liriope and Ophiopogon taxa have made the taxonomy of the two genera problematic and caused confusion about the identification of individual specimens. Molecular approaches could be a useful tool for the discrimination of these two genera in combination with traditional methods. Seventy-five Liriope and Ophiopogon samples from the UK National Plant Collections of Ophiopogon and Liriope were analyzed. The 5′ end of the DNA barcode region of the gene for the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (rbcLa) was used for the discrimination of the two genera. A single nucleotide polymorphism (SNP) between the two genera allowed the development of discriminatory tests for genus-level identification based on specific PCR and high-resolution melt curve (HRM) assays. The study highlights the advantage of incorporating DNA barcoding methods into plant identification protocols and provides simple assays that could be used for the quality assurance of commercially traded plants and herbal drugs. View Full-Text
Keywords: Ophiopogon; Liriope; rbcL; DNA barcoding; high-resolution melt curve (HRM) analysis Ophiopogon; Liriope; rbcL; DNA barcoding; high-resolution melt curve (HRM) analysis
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Masiero, E.; Banik, D.; Abson, J.; Greene, P.; Slater, A.; Sgamma, T. Genus-Specific Real-Time PCR and HRM Assays to Distinguish Liriope from Ophiopogon Samples. Plants 2017, 6, 53.

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