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Plants 2017, 6(2), 20;

Attenuation of Drought Stress in Brassica Seedlings with Exogenous Application of Ca2+ and H2O2

Institute of Biotechnology and Genetic Engineering, University of Agriculture, Peshawar 25120, Pakistan
Department of Biotechnology, Bacha Khan University Charsadda, Charsadda 24420, Pakistan
Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia
Department of Agronomy, Faculty of Agriculture, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka 1207, Bangladesh
Author to whom correspondence should be addressed.
Academic Editor: Masayuki Fujita
Received: 30 January 2017 / Revised: 8 May 2017 / Accepted: 10 May 2017 / Published: 13 May 2017
(This article belongs to the Special Issue Abiotic Environmental Stress Responses of Plants)
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Drought is one of the most common abiotic stresses, affecting the growth and productivity of crop plants globally, particularly in arid and semi-arid regions. Different strategies are used to mitigate the impact of drought among crop plants. Exogenous application of different substances are known to decrease the effects of various abiotic stresses, including drought stress. The aim of this study was to evaluate the effect of Ca2+ and H2O2 in developing drought stress tolerance in Brassica napus “Bulbul-98” seedlings. Brassica napus “Bulbul-98” seedlings were exposed to 5, 10 and 15 mM Ca2+ and 2, 5 and 10 μM H2O2 concentrations twice at an interval of two days for up to 20 days after germination. Drought stress decreased relative water content (RWC), chlorophyll content and increased proline, H2O2, soluble protein and electrolyte leakage in Brassica seedlings. Exogenous Ca2+ (5, 10,15 mM) and H2O2 (2, 5, 10 μM) supplementations, during drought stress induction, showed a significant increase in RWC by 5.4%, 18.06%, 26.2% and 6.87%, 13.9%, 18.3% respectively. Similarly, with the exogenous application of Ca2+ (5, 10, 15 mM) and H2O2 (2, 5, 10 μM), chlorophyll content was increased by 15.03%, 22.2%, and 28.4%, and 9.6%, 23.3%, and 27.5% respectively. It was confirmed that the seedlings under drought stress that were supplemented with Ca2+ and H2O2 recovered from water content reduction and chlorosis, and were able to grow normally. View Full-Text
Keywords: drought stress; relative water content; electrolyte leakage; proline; SDS-PAGE drought stress; relative water content; electrolyte leakage; proline; SDS-PAGE

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Khan, A.; Anwar, Y.; Hasan, M.M.; Iqbal, A.; Ali, M.; Alharby, H.F.; Hakeem, K.R.; Hasanuzzaman, M. Attenuation of Drought Stress in Brassica Seedlings with Exogenous Application of Ca2+ and H2O2. Plants 2017, 6, 20.

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