The Effects of Salt Stress on Germination, Seedling Growth and Biochemical Responses of Tunisian Squash (Cucurbita maxima Duchesne) Germplasm
Abstract
:1. Introduction
2. Results
2.1. Effect of Salt Stress Level on Traits Related to Seed Germination and Seedling Growth Potential
2.2. Effect of Landrace on Traits Related to Seed Germination and Seedling Growth Potential
2.3. Effect of the Landrace and the Salt Stress Level on Traits Related to Seed Germination and Seedling Growth Potential
2.4. Effect of the Salt Stress Level on the Content of Malondialdehyde (MDA), Free Proline and Chlorophyll a and b
2.5. Effect of Landrace on the Content of Malondialdehyde, Free Proline and Chlorophyll a and b
2.6. Effect of Landrace and Salt Stress Level on the Content of Malondialdehyde, Free Proline and Chlorophyll a and b
3. Discussion
4. Materials and Methods
4.1. Plant Material and Growth Conditions
4.2. Salinity Stress Treatments
4.3. Determination of Germination and Seedling Growth Potential under Salt Stress
4.4. Evaluation of Salinity Tolerance Based on Biochemical Parameters
4.5. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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S.O.V. | DF | GP | SL | RL | SFW | RFW | SRR | GR | SLR | RLR | GSTI | SLSTI | RLSTI |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Accession | 14 | 4066.8 | 341.0 | 152.9 | 2.6 | 0.05 | 54.8 | 3801.8 | 487.9 | 115.3 | 0.39 | 26,109.5 | 18,310.1 |
Salinity | 3 | 17,405.8 | 1103.6 | 697.3 | 8.4 | 0.25 | 139.3 | 7553.0 | 22.9 | 227.9 | 0.98 | 1484.6 | 44,510.7 |
Accession x Salinity | 42 | 620.4 | 46.7 | 14.1 | 0.2 | 0.004 | 18.9 | 455.4 | 9.1 | 6.6 | 0.05 | 669.1 | 1321.4 |
CV (%) | 13.3 | 9.0 | 11.3 | 17.0 | 24.9 | 20.6 | 10.2 | 20.7 | 21.7 | 29.69 | 15.4 | 16.6 |
NaCl Concentration (mM) | GP (%) | SL (mm) | RL (mm) | SFW (g) | RFW (g) | SRR | GR (%) | SLR (%) | RRL (%) | GSTI (%) | SLSTI (%) | RLSTI (%) |
---|---|---|---|---|---|---|---|---|---|---|---|---|
Control | 86.3 ± 1.08 a | 12.3 ± 0.24 a | 7.4 ± 0.18 a | 0.9 ± 0.02 a | 0.14 a ± 0.004 a | 1.3 ± 0.02 c | - | - | - | - | - | - |
100 | 23.3 ± 1.50 b | 7.1 ± 0.33 b | 4.7 ± 0.24 b | 0.6 ± 0.03 b | 0.08 ± 0.004 b | 1.7 ± 0.06 b | 63.0 ± 1.49 c | 5.2 ± 0.37 b | 2.7 ± 0.22 c | 0.27 ± 0.016 a | 60.1 ± 2.83 a | 63.8 ± 2.94 a |
200 | 15.0 ± 1.08 c | 6.4 ± 0.31 c | 3.3 ± 0.20 c | 0.5 ± 0.05 c | 0.06 ± 0.003 c | 1.8 ± 0.10 b | 71.3 ± 1.18 b | 5.9 ± 0.35 a | 4.1 ± 0.19 b | 0.17 ± 0.011 b | 54.2 ± 2.58 b | 44.0 ± 2.34 b |
300 | 8.4 ± 0.67 d | 6.3 ± 0.30 c | 2.0 ± 0.14 d | 0.3 ± 0.01 d | 0.04 ± 0.003 d | 3.6 ± 0.27 a | 77.9 a ± 1.02 a | 5.9 ± 0.39 a | 5.3 ± 0.16 a | 0.09 ± 0.006 v | 54.6 ± 2.84 b | 27.5 ± 1.77 c |
F Value | 8816 ** | 2141.20 ** | 2879.11 ** | 815.17 ** | 649.38 ** | 745.38 ** | 362.56 ** | 143.95 ** | 791.03 ** | 296.89 ** | 19.75 ** | 16.58 ** |
Accession | GP (%) | SL (mm) | RL (mm) | SFW (g) | RFW (g) | SRR | GR (%) | SLR (%) | RRL (%) | GSTI (%) | SLSTI (%) | RLSTI (%) |
---|---|---|---|---|---|---|---|---|---|---|---|---|
“745” | 42.0 ± 4.72 bc | 8.0 ± 0.18 gh | 4.7 ± 0.29 de | 0.6 ± 0.02 de | 0.07 ± 0.008 e | 1.8 ± 0.58 h | 74.6 ± 3.55 c | 6.0 ± 1.11 d | 2.0 ± 0.56 h | 0.24 ± 0.04 e | 56.67 ± 7.45 e | 68.9 ± 7.47 b |
“746” | 48.4 ± 5.36 a | 8.3 ± 0.32 g | 4.5 ± 0.24 e | 0.6 ± 0.007 de | 0.02 ± 0.005 h | 2.4 ± 0.26 e | 67.0 ± 3.15 de | 2.1 ± 0.69 h | 3.6 ± 0.74 e | 0.32 ± 0.03 ab | 80.85 ± 5.66 a | 49.8 ± 7.55 e |
“747” | 49.3 ± 4.81 a | 7.8 ± 0.16 h | 3.0 ± 0.26 g | 0.6 ± 0.03 de | 0.03 ± 0.005 fg | 3.6 ± 0.36 b | 64.0 ± 2.64 ef | 1.6 ± 0.25 h | 2.5 ± 0.61 fh | 0.34 ± 0.07 a | 82.25 ± 7.23 a | 44.3 ± 8.50 ef |
“748” | 41.5 ± 7.02 cd | 9.3 ± 1.11 d | 4.7 ± 0.66 de | 0.8 ± 0.05 b | 0.10 ± 0.008 cd | 2.1 ± 0.72 f | 64.6 ± 2.49 ef | 4.8 ± 0.89 e | 2.0 ± 0.55 gh | 0.29 ± 0.02 cd | 64.20 ± 5.15 d | 67.6 ± 8.47 b |
“749” | 40.0 ± 4.93 cd | 9.3 ± 0.29 de | 4.0 ± 0.41 f | 0.6 ef ± 0.01 ef | 0.10 ± 0.006 cd | 2.9 ± 0.32 d | 60.0 ± 3.56 g | 4.1 ± 0.81 f | 4.1 ± 0.64 d | 0.30 ± 0.02 bc | 72.54 ± 6.80 b | 42.9 ± 7.89 gh |
“750” | 39.5 ± 5.05 d | 5.3 ± 0.81 i | 3.0 ± 0.51 g | 0.8 ± 0.05 c | 0.10 ± 0.006 cd | 3.3 ± 0.24 c | 71.3 ± 4.40 c | 2.0 ± 0.66 h | 2.8 ± 0.52 f | 0.23 ± 0.03 e | 73.00 ± 6.77 b | 44.7 ± 8.12 fg |
“751” | 31.2 ± 4.83 e | 9.8 ± 0.35 c | 8.0 ± 0.36 b | 0.9 ± 0.02 b | 0.09 ± 0.004 d | 1.2 ± 0.23 i | 65.0 ± 5.06 ef | 10.6 ± 1.35 c | 5.3 ± 0.79 c | 0.19 ± 0.05 f | 40.54 ± 5.44 f | 57.4 ± 7.36 c |
“752” | 42.7 ± 4.10 b | 8.8 ± 0.37 f | 5.8 ± 0.36 c | 1.1 ± 0.03 a | 0.15 ± 0.006 a | 1.5 ± 0.34 h | 71.0 ± 2.64 cd | 3.8 ± 0.85 f | 2.3 ± 0.50 gh | 0.26 ± 0.02 de | 67.43 ± 6.70 cd | 70.5 ± 5.47 b |
“753” | 28.7 ± 5.47 f | 9.0 ± 0.24 ef | 3.0 ± 0.31 g | 0.6 ± 0.05 d | 0.11 ± 0.005 b | 4.5 ± 0.52 a | 61.7 ± 4.04 fg | 3.0 ± 0.80 h | 3.4 ± 0.71 e | 0.18 ± 0.04 f | 81.11 ± 8.30 a | 32.6 ± 4.55 h |
“1004” | 23.2 ± 5.09 gh | 10.9 ± 0.24 b | 4.7 ± 0.57 d | 0.5 ± 0.02 f | 0.10 ± 0.006 cd | 3.1 ± 0.21 c | 66.3 ± 3.20 e | 3.6 ± 0.84 f | 4.1 ± 0.82 d | 0.09 ± 0.03 h | 73.98 ± 6.21 b | 50.8 ± 8.24 de |
“1005” | 22.0 ± 3.23 gh | 10.7 ± 0.22 b | 5.9 ± 0.62 c | 0.6 ± 0.04 de | 0.10 ± 0.006 cd | 1.9 ± 0.18 fg | 54.7 ± 6.80 h | 4.2 ± 0.91 f | 4.2 ± 0.80 d | 0.13 ± 0.20 g | 69.99 ± 5.41 bc | 54.6 ± 4.91 cd |
“1006” | 28.1 ± 4.95 f | 13.4 ± 0.85 a | 8.3 ± 0.38 a | 0.4 ± 0.03 g | 0.10 ± 0.006 cd | 1.7 ± 0.16 gh | 92.3 ± 2.77 a | 2.8 ± 0.60 g | 2.1 ± 0.58 gh | 0.15 ± 0.01 i | 81.98 ± 7.16 a | 81.6 ± 7.93 a |
“1007” | 21.5 ± 6.76 h | 2.7 ± 0.34 k | 1.8 ± 0.30 i | 0.2 ± 0.03 h | 0.04 ± 0.007 f | 0.4 ± 0.10 j | 86.0 ± 2.58 b | 10.7 ± 1.42 c | 7.4 ± 0.88 b | 0.03 ± 0.01 i | 0.00 ± 0.00 g | 0.0 ± 0.00 i |
“1008” | 16.7 ± 6.30 i | 2.9 ± 0.78 k | 1.3 ± 0.54 j | 0.1 ± 0.06 i | 0.03 ± 0.001 h | 0.5 ± 0.13 j | 67.0 ± 4.99 de | 11.6 ± 1.54 b | 5.3 ± 0.84 c | 0.04 ± 0.01 i | 0.00 ± 0.00 g | 0.0 ± 0.00 i |
“1009” | 24.0 ± 4.94 g | 3.8 ± 0.38 j | 2.3 ± 0.21 h | 0.2 ± 0.04 h | 0.03 ± 0.006 h | 0.4 ± 0.10 j | 96.0 ± 4.40 a | 15.1 ± 1.66 a | 9.1 ± 0.96 a | 0.03 ± 0.01 i | 0.00 ± 0.00 g | 0.0 ± 0.00 i |
CV % | 13.54 | 8.96 | 11.32 | 16.98 | 23.92 | 20.63 | 10.23 | 20.69 | 21.74 | 26.69 | 15.39 | 16.62 |
NaCl Concentration (mM) | Accession | GP (%) | SL (mm) | RL (mm) | SFW (g) | RFW (g) | SRR |
---|---|---|---|---|---|---|---|
Control | “745” | 97.9 ± 1.5 | 12.5 ± 1.6 | 6.2 ± 0.8 | 0.9 ± 0.2 | 0.15 ± 0.02 | 2.0 ± 0.4 |
“746” | 98.7 ± 1.5 | 9.9 ± 1.8 | 7.2 ± 0.7 | 0.8 ± 0.1 | 0.04 ± 0.01 | 1.4 ± 0.2 | |
“747” | 97.3 ± 2.3 | 9.1 ± 0.7 | 4.9 ± 0.5 | 0.7 ± 0.4 | 0.07 ± 0.03 | 1.9 ± 0.2 | |
“748” | 90.0 ± 8.7 | 12.9 ± 1.8 | 6.2 ± 0.6 | 1.1 ± 0.3 | 0.15 ± 0.02 | 2.1 ± 0.4 | |
“749” | 85.0 ± 4.3 | 12.4 ± 1.6 | 7.0 ± 1.2 | 0.76 ± 0.06 | 0.15 ± 0.01 | 1.8 ± 0.4 | |
“750” | 93.0 ± 2.6 | 6.8 ± 0.7 | 5.1 ± 0.3 | 1.1 ± 0.1 | 0.16 ± 0.02 | 1.3 ± 0.2 | |
“751” | 80.0 ± 4.3 | 17.8 ± 1.8 | 12.0 ± 2.0 | 1.3 ± 0.2 | 0.14 ± 0.02 | 1.5 ± 0.1 | |
“752” | 96.0 ± 2.6 | 11.7 ± 1.1 | 7.5 ± 0.6 | 1.7 ± 0.2 | 0.19 ± 0.05 | 1.4 ± 0.2 | |
“753” | 75.0 ± 4.3 | 10.4 ± 0.4 | 5.6 ± 0.3 | 0.83 ± 0.03 | 0.18 ± 0.02 | 1.9 ± 0.1 | |
“1004” | 73.0 ± 5.7 | 13.5 ± 1.1 | 7.7 ± 1.4 | 0.63 ± 0.05 | 0.15 ± 0.01 | 1.8 ± 0.3 | |
“1005” | 63.0 ± 6.7 | 13.9 ± 1.0 | 9.1 ± 1.1 | 0.8 ± 0.1 | 0.15 ± 0.03 | 1.5 ± 0.2 | |
“1006” | 97.3 ± 1.9 | 15.5 ± 0.5 | 9.9 ± 0.4 | 0.69 ± 0.07 | 0.16 ± 0.02 | 1.6 ± 0.1 | |
“1007” | 86.0 ± 5.2 | 10.7 ± 0.4 | 7.4 ± 0.4 | 0.86 ± 0.03 | 0.17 ± 0.02 | 1.4 ± 0.1 | |
“1008” | 67.0 ± 5.7 | 11.6 ± 0.8 | 5.3 ± 0.3 | 0.52 ± 0.03 | 0.16 ± 0.02 | 2.2 ± 0.2 | |
“1009” | 96.0 ± 2.6 | 15.1 ± 0.8 | 9.1 ± 0.3 | 0.73 ± 0.09 | 0.10 ± 0.01 | 1.7 ± 0.1 | |
100 | “745” | 50.0 ± 5.7 | 7.7 ± 0.6 | 5.0 ± 0.5 | 0.7 ± 0.04 | 0.08 ± 0.01 | 1.5 ± 0.2 |
“746” | 40.0 ± 4.3 | 9.0 ± 0.6 | 5.7 ± 0.5 | 0.6 ± 0.2 | 0.03 ± 0.05 | 1.6 ± 0.2 | |
“747” | 45.0 ± 4.3 | 8.0 ± 0.4 | 3.8 ± 0.4 | 0.68 ± 0.04 | 0.04 ± 0.01 | 2.1 ± 0.2 | |
“748” | 35.0 ± 3.3 | 8.6 ± 0.5 | 5.3 ± 0.2 | 0.9 ± 0.2 | 0.10 ± 0.03 | 1.6 ± 0.1 | |
“749” | 40.0 ± 4.8 | 9.0 ± 0.9 | 4.4 ± 0.3 | 0.64 ± 0.03 | 0.10 ± 0.01 | 2.0 ± 0.3 | |
“750” | 30.0 ± 5.3 | 4.8 ± 0.9 | 4.5 ± 0.3 | 0.92 ± 0.06 | 0.11 ± 0.01 | 1.1 ± 0.2 | |
“751” | 30.0 ± 5.3 | 8.5 ± 0.5 | 9.3 ± 0.3 | 1.00 ± 0.04 | 0.11 ± 0.01 | 0.9 ± 0.1 | |
“752” | 35.0 ± 4.3 | 8.9 ± 0.5 | 6.3 ± 0.3 | 1.29 ± 0.08 | 0.17 ± 0.01 | 1.4 ± 0.1 | |
“753” | 25.0 ± 3.3 | 7.6 ± 0.4 | 3.5 ± 0.3 | 0.71 ± 0.04 | 0.12 ± 0.01 | 2.2 ± 0.2 | |
“1004” | 25.0 ± 3.3 | 10.4 ± 0.7 | 6.2 ± 0.3 | 0.58 ± 0.02 | 0.11 ± 0.01 | 1.7 ± 0.1 | |
“1005” | 15.0 ± 2.6 | 11.1 ± 0.8 | 6.5 ± 0.2 | 0.69 ± 0.02 | 0.10 ± 0.01 | 1.7 ± 0.2 | |
“1006” | 10.0 ± 4.4 | 12.2 ± 0.5 | 9.4 ± 0.3 | 0.44 ± 0.03 | 0.10 ± 0.01 | 1.3 ± 0.1 | |
“1007” | 0 | 0 | 0 | 0 | 0 | 0 | |
“1008” | 0 | 0 | 0 | 0 | 0 | 0 | |
“1009” | 0 | 0 | 0 | 0 | 0 | 0 | |
200 | “745” | 15.0 ± 4.3 | 6.6 ± 0.4 | 4.1 ± 0.2 | 0.53 ± 0.03 | 0.04 ± 0.00 | 1.6 ± 0.1 |
“746” | 35.0 ± 4.3 | 7.7 ± 0.6 | 3.4 ± 0.3 | 0.50 ± 0.04 | 0.01 ± 0.00 | 2.3 ± 0.3 | |
“747” | 35.0 ± 4.3 | 7.5 ± 0.4 | 2.2 ± 0.3 | 0.55 ± 0.03 | 0.01 ± 0.00 | 3.5 ± 0.5 | |
“748” | 25.0 ± 3.2 | 8.2 ± 0.3 | 4.1 ± 0.2 | 0.74 ± 0.03 | 0.08 ± 0.03 | 2.0 ± 0.1 | |
“749” | 25.0 ± 3.2 | 8.2 ± 0.7 | 3.0 ± 0.3 | 0.49 ± 0.14 | 0.08 ± 0.01 | 2.8 ± 0.3 | |
“750” | 25.0 ± 3.2 | 3.7 ± 0.5 | 1.6 ± 0.2 | 0.64 ± 0.02 | 0.08 ± 0.01 | 2.3 ± 0.3 | |
“751” | 10.0 ± 4.2 | 7.2 ± 0.4 | 6.4 ± 0.3 | 0.85 ± 0.04 | 0.08 ± 0.01 | 1.1 ± 0.1 | |
“752” | 25.0 ± 3.3 | 7.6 ± 0.4 | 5.9 ± 0.3 | 0.88 ± 0.03 | 0.13 ± 0.01 | 1.3 ± 0.1 | |
“753” | 10.0 ± 3.3 | 8.3 ± 0.3 | 2.2 ± 0.2 | 0.57 ± 0.03 | 0.09 ± 0.03 | 3.9 ± 0.3 | |
“1004” | 5.0 ± 1.7 | 9.8 ± 0.7 | 3.4 ± 0.3 | 0.53 ± 0.02 | 0.08 ± 0.01 | 2.9 ± 0.4 | |
“1005” | 10.0 ± 4.2 | 9.8 ± 0.3 | 4.7 ± 0.1 | 0.51 ± 0.03 | 0.08 ± 0.01 | 2.1 ± 0.1 | |
“1006” | 5.0 ± 1.7 | 10.8 ± 0.5 | 8.5 ± 0.4 | 0.33 ± 0.02 | 0.08 ± 0.01 | 1.3 ± 0.1 | |
“1007” | 0 | 0 | 0 | 0 | 0 | 0 | |
“1008” | 0 | 0 | 0 | 0 | 0 | 0 | |
“1009” | 0 | 0 | 0 | 0 | 0 | 0 | |
300 | “745” | 5.0 ± 1.7 | 5.3 ± 0.6 | 3.5 ± 0.3 | 0.33 ± 0.03 | 0.02 ± 0.00 | 1.5 ± 0.2 |
“746” | 20.0 ± 3.3 | 6.7 ± 0.7 | 1.6 ± 0.3 | 0.50 ± 0.01 | 0.01 ± 0.00 | 4.0 ± 0.9 | |
“747” | 25.0 ± 3.3 | 6.7 ± 0.4 | 1.0 ± 0.2 | 0.47 ± 0.03 | 0.01 ± 0.00 | 7.0 ± 1.4 | |
“748” | 17.0 ± 4.1 | 7.7 ± 0.3 | 3.0 ± 0.3 | 0.5 ± 0.1 | 0.07 ± 0.01 | 2.6 ± 0.2 | |
“749” | 10.0 ± 3.2 | 7.5 ± 0.5 | 1.6 ± 0.2 | 0.35 ± 0.03 | 0.05 ± 0.01 | 5.0 ± 1.0 | |
“750” | 10.0 ± 3.2 | 6.0 ± 1.0 | 0.7 ± 0.1 | 0.32 ± 0.04 | 0.06 ± 0.02 | 8.5 ± 1.3 | |
“751” | 5.0 ± 1.7 | 5.6 ± 0.6 | 4.4 ± 0.3 | 0.35 ± 0.03 | 0.04 ± 0.01 | 1.3 ± 0.1 | |
“752” | 15.0 ± 4.3 | 6.9 ± 0.4 | 3.6 ± 0.2 | 0.64 ± 0.03 | 0.12 ± 0.01 | 1.9 ± 0.1 | |
“753” | 5.0 ± 1.7 | 9.4 ± 0.3 | 0.9 ± 0.1 | 0.38 ± 0.02 | 0.05 ± 0.02 | 10.3 ± 1.5 | |
“1004” | 5.0 ± 1.7 | 9.8 ± 0.7 | 1.6 ± 0.2 | 0.35 ± 0.02 | 0.05 ± 0.01 | 6.2 ± 0.8 | |
“1005” | 5.0 ± 1.7 | 8.1 ± 0.5 | 3.5 ± 0.2 | 0.3 ± 0.1 | 0.05 ± 0.03 | 2.3 ± 0.1 | |
“1006” | 5.0 ± 1.7 | 15.2 ± 0.5 | 5.4 ± 0.3 | 0.21 ± 0.02 | 0.06 ± 0.02 | 2.8 ± 0.1 | |
“1007” | 0 | 0 | 0 | 0 | 0 | 0 | |
“1008” | 0 | 0 | 0 | 0 | 0 | 0 | |
“1009” | 0 | 0 | 0 | 0 | 0 | 0 |
NaCl concentration (mM) | MDA (µmol g−1) | Proline (µg mg−1) | Chl a (mg mg−1) | Chl b (mg mg−1) |
---|---|---|---|---|
Control | 1.17 ± 0.53 b | 0.82 ± 0.06 a | 30.09 ± 2.87 b | 60.64 ± 5.96 b |
100 | 1.75 ± 0.30 a | 0.51 ± 0.02 b | 35.47 ± 3.90 a | 69.88 ± 7.68 a |
200 | 1.43 ± 0.21 b | 0.82 ± 0.04 a | 25.70 ± 2.43 d | 52.44 ± 5.50 d |
300 | 1.34 ± 0.18 b | 0.92 ± 0.05 a | 28.91 ± 2.46 c | 56.34 ± 5.98 c |
F Value | 33.6 ** | 471.2 ** | 552.3 ** | 737.5 ** |
SD |
Accession | MDA (µmol g−1) | Proline (µg mg−1) | Chl a (mg mg−1) | Chl b (mg mg−1) |
---|---|---|---|---|
“746” | 1.6 ± 0.12 a | 1.0 ± 0.06 a | 12.5 ± 1.50 d | 25.7 ± 2.08 d |
“747” | 0.8 ± 0.19 b | 0.8 ± 0.02 b | 14.8 ± 1.16 c | 27.9 ± 2.15 c |
“748” | 0.8 ± 0.12 b | 0.5 ± 0.04 d | 41.8 ± 1.50 b | 77.0 ± 2.08 b |
“751” | 0.9 ± 0.26 b | 0.6 ± 0.05 c | 51.1 ± 1.29 a | 108.7 ± 3.82 a |
F Value | 15.5 ** | 1466.3 ** | 12473.9 ** | 21337.9 ** |
SD |
Accession | NaCl Concentration (mM) | MDA (µmol g−1) | Proline (µg mg−1) | Chl a (mg mg−1) | Chl b (mg mg−1) |
---|---|---|---|---|---|
“746” | Control | 0.88 ± 0.03 | 1.42 ± 0.03 | 14.7 ± 0.2 | 31.1 ± 0.7 |
100 | 4.24 ± 0.03 | 0.74 ± 0.01 | 11.6 ± 0.2 | 24.4 ± 0.5 | |
200 | 0.58 ± 0.08 | 1.13 ± 0.05 | 9.9 ± 0.2 | 20.7 ± 0.3 | |
300 | 0.68 ± 0.09 | 0.71 ± 0.08 | 13.7 ± 0.1 | 26.6 ± 0.8 | |
“747” | Control | 0.9 ± 0.1 | 0.59 ± 0.02 | 13.7 ± 0.1 | 26.0 ± 0.3 |
100 | 1.9 ± 0.6 | 0.44 ± 0.01 | 13.2 ± 0.2 | 25.8 ± 0.2 | |
200 | 0.36 ± 0.06 | 0.56 ± 0.02 | 15.6 ± 0.4 | 29.5 ± 0.5 | |
300 | 0.5 ± 0.2 | 0.8 ± 0.009 | 16.8 ± 0.4 | 30.2 ± 1.1 | |
“748” | Control | 1.8 ± 0.4 | 0.47 ± 0.05 | 37.5 ± 0.6 | 72.2 ± 0.9 |
100 | 0.65 ± 0.03 | 0.48 ± 0.04 | 57.0 ± 0.8 | 103.6 ± 1.5 | |
200 | 0.60 ± 0.04 | 0.55 ± 0.05 | 30.2 ± 0.6 | 55.2 ± 0.6 | |
300 | 0.20 ± 0.07 | 0.40 ± 0.01 | 42.5 ± 0.8 | 76.9 ± 0.9 | |
“751” | Control | 1.1 ± 0.2 | 0.63 ± 0.09 | 54.5 ± 0.5 | 113.3 ± 1.7 |
100 | 0.2 ± 0.1 | 0.46 ± 0.01 | 60.0 ± 3.8 | 125.7 ± 3.9 | |
200 | 0.20 ± 0.03 | 1.06 ± 0.05 | 47.1 ± 0.4 | 104.3 ± 3.5 | |
300 | 1.56 ± 0.20 | 0.98 ± 0.03 | 42.7 ± 0.3 | 91.7 ± 2.4 |
Landrace Inventory Number | Local Name | Origin | Latitude | Longitude | Short Description |
---|---|---|---|---|---|
NGBTUN745 (“745”) | Batati Green | Ariana (Kalaat Andalous) | 37°033″ N | 10°11′7″ E | Globular fruit, light green skin, green flesh |
NGBTUN746 (“746”) | Batati orange | Siliana (SidiHamada) | 35°57′28″ N | 9°32′57″ E | Globular fruit, orange skin, light orange flesh |
NGBTUN747 (“747”) | Galaoui | Ariana (Kalaa Andalous) | 37°033″ N | 10°11′7″ E | Raised fruit with basal tip, green skin, green flesh |
NGBTUN748 (“748”) | Karkoubi orange | Sousse (SidiBouali) | 35°54′22.21″ N | 10°32′47.81″ E | Flattened fruit, dark yellow skin, yellow flesh |
NGBTUN749 (“749”) | Batati yellow spotted with white | Siliana (SidiHamada) | 35°57′28″ N | 9°32′57″ E | Globular fruit, orange skin spotted with white, orange flesh |
NGBTUN750 (“750”) | Batati white | Monastir (Sahline) | 35°45′05″ N | 10°42′39″ E | Globular fruit, white skin, white flesh |
NGBTUN751 (“751”) | Bejaoui Green | Siliana (SidiHamada) | 35°57′28″ N | 9°32′57″ E | Flattened fruit, dark green skin, light green flesh |
NGBTUN752 (“752”) | Batati yellow | Siliana (North) | 35°57′28″ N | 9°32′57″ E | Globular fruit, yellow skin, light orange flesh |
NGBTUN753 (“753”) | Béjaoui Green | Siliana (South) | 35°57′28″ N | 9°32′57″ E | Flattened fruit, dark green skin, light green flesh |
NGBTUN1004 (“1004”) | Galaoui large seeds | Ariana (Kalaat Andalous) | 37°033″ N | 10°11′7″ E | Turbinate interior fruit with basal tip, green skin, white green flesh |
NGBTUN1005 (“1005”) | Galaoui smoll seeds | Ariana (Kalaat Andalous) | 37°033″ N | 10°11′7″ E | Turbinate interior fruit with a big basal tip, green skin, white green flesh |
NGBTUN1006 (“1006”) | Karkoubi orange | Monastir (Sahline) | 35°45′05″ N | 10°42′39″ E | Flattened fruit, dark yellow skin, yellow flesh |
NGBTUN1007 (“1007”) | Batati Green | Siliana | 35°57′28″ N | 9°32′57″ E | Rounded fruit, green skin, green flesh |
NGBTUN1008 (“1008”) | Batati Green | Monastir (Teboulba) | 35°45′05″ N | 10°42′39″ E | Globular fruit, flat stem end, green skin, light green flesh |
NGBTUN1009 (“1009”) | Bejaoui spotted with yellow | Siliana (SidiHamada) | 35°57′28″ N | 9°32′57″ E | Globular fruit with flat stem end, spotted with yellow light green skin, light green flesh |
Trait | Unit | Description/Formula | Reference |
---|---|---|---|
Germination percentage (GP) | % | Scott et al. (1984) | |
Shoot length (SL) | mm | At the day of germination | Sivakumar et al. (2020) |
Root length (RL) | mm | At the day of germination | Sivakumar et al. (2020) |
Shoot fresh weight (SFW) | g | Recorded by using a sensitive balance (Sartorius AC 1215, Germany) | Jamil et al. (2006) |
Root fresh weight (RFW) | g | Recorded by using a sensitive balance (Sartorius AC 1215, Germany) | Jamil et al. (2006) |
Shoot length/Root length Ratio (SRR) | - | Ratio of SL to RL | Thabet et al. (2018) |
Germination reduction (GR) | % | GR = GP of controls − GP of stress plants | Thabet et al. (2018) |
Shoot length reduction (SLR) | mm | SLR = SL of controls − SL of stress plants | Partheeban et al. (2017) |
Root length reduction (RLR) | mm | RLR = RL of controls − RL of stress plants | Thabet et al. (2018) |
Germination stress tolerance index (GSTI) | % | Partheeban et al. (2017) | |
Shoot length stress tolerance index (SLSTI) | % | Partheeban et al. (2017) | |
Root length stress tolerance index (RLSTI) | % | Partheeban et al. (2017) |
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Tarchoun, N.; Saadaoui, W.; Mezghani, N.; Pavli, O.I.; Falleh, H.; Petropoulos, S.A. The Effects of Salt Stress on Germination, Seedling Growth and Biochemical Responses of Tunisian Squash (Cucurbita maxima Duchesne) Germplasm. Plants 2022, 11, 800. https://doi.org/10.3390/plants11060800
Tarchoun N, Saadaoui W, Mezghani N, Pavli OI, Falleh H, Petropoulos SA. The Effects of Salt Stress on Germination, Seedling Growth and Biochemical Responses of Tunisian Squash (Cucurbita maxima Duchesne) Germplasm. Plants. 2022; 11(6):800. https://doi.org/10.3390/plants11060800
Chicago/Turabian StyleTarchoun, Neji, Wassim Saadaoui, Najla Mezghani, Ourania I. Pavli, Hanen Falleh, and Spyridon A. Petropoulos. 2022. "The Effects of Salt Stress on Germination, Seedling Growth and Biochemical Responses of Tunisian Squash (Cucurbita maxima Duchesne) Germplasm" Plants 11, no. 6: 800. https://doi.org/10.3390/plants11060800