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Article

Isolation and Characterization of a Novel Pathogenesis-Related Protein-1 Gene (AvPR-1) with Induced Expression in Oat (Avena sativa L.) during Abiotic and Hormonal Stresses

by
Khalid A. AlHudaib
1,2,*,
Naimah Asid Alanazi
3,
Mouna Ghorbel
3,
Sherif Mohamed El-Ganainy
1,2 and
Faiçal Brini
4
1
Department of Arid Land Agriculture, College of Agriculture and Food Sciences, King Faisal University, P.O. Box 420, Al-Ahsa 31982, Saudi Arabia
2
Pests and Plant Diseases Unit, College of Agriculture and Food Sciences, King Faisal University, P.O. Box 420, Al-Ahsa 31982, Saudi Arabia
3
Department of Biology, College of Sciences, University of Hail, P.O. Box 2440, Ha’il City 81451, Saudi Arabia
4
Laboratory of Biotechnology and Plant Improvement, Center of Biotechnology of Sfax, University of Sfax, P.O. Box 1177, Sfax 3018, Tunisia
*
Author to whom correspondence should be addressed.
Plants 2022, 11(17), 2284; https://doi.org/10.3390/plants11172284
Submission received: 6 August 2022 / Revised: 20 August 2022 / Accepted: 27 August 2022 / Published: 31 August 2022
(This article belongs to the Special Issue Responses of Plants to Environmental Stresses Volume II)
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Abstract

Pathogenesis-related protein-1 (PR-1) plays crucial roles in regulating plant responses to biotic and abiotic stresses. This study aimed to isolate and characterize the first PR-1 (AvPR-1) gene in oat (Avena sativa L.). AvPR-1 presented conserved signal peptide motifs and core amino acid composition in the functional protein domains as the protein sequence of AvPR-1 presented 98.28%, 97.7%, and 95.4% identity with known PR1 proteins isolated from Triticum aestivum PRB1-2-like, Triticum dicoccoides PRB1-2-like, and Aegilops tauschii subsp. tauschii, respectively. Bioinformatic analysis showed that the AvPR-1 protein belongs to the CAP superfamily (PF00188). Secondary and 3D structure analyses of the AvPR-1 protein were also conducted, confirming sequence conservation of PR-1 among studied species. The AvPR-1 protein harbors a calmodulin-binding domain located in its C-terminal part as previously shown for its wheat homolog TdPR1.2. Moreover, gene expression analysis showed that AvPR-1 was induced in response to many abiotic and hormonal stresses especially in leaves after treatment for 48 h. This is the first study exhibiting the expression profiles of the AvPR-1 gene under different stresses in oat.
Keywords: environmental stress; Avena sativa; bioinformatic analysis; gene expression; pathogenesis-related proteins; phytohormones environmental stress; Avena sativa; bioinformatic analysis; gene expression; pathogenesis-related proteins; phytohormones

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MDPI and ACS Style

AlHudaib, K.A.; Alanazi, N.A.; Ghorbel, M.; El-Ganainy, S.M.; Brini, F. Isolation and Characterization of a Novel Pathogenesis-Related Protein-1 Gene (AvPR-1) with Induced Expression in Oat (Avena sativa L.) during Abiotic and Hormonal Stresses. Plants 2022, 11, 2284. https://doi.org/10.3390/plants11172284

AMA Style

AlHudaib KA, Alanazi NA, Ghorbel M, El-Ganainy SM, Brini F. Isolation and Characterization of a Novel Pathogenesis-Related Protein-1 Gene (AvPR-1) with Induced Expression in Oat (Avena sativa L.) during Abiotic and Hormonal Stresses. Plants. 2022; 11(17):2284. https://doi.org/10.3390/plants11172284

Chicago/Turabian Style

AlHudaib, Khalid A., Naimah Asid Alanazi, Mouna Ghorbel, Sherif Mohamed El-Ganainy, and Faiçal Brini. 2022. "Isolation and Characterization of a Novel Pathogenesis-Related Protein-1 Gene (AvPR-1) with Induced Expression in Oat (Avena sativa L.) during Abiotic and Hormonal Stresses" Plants 11, no. 17: 2284. https://doi.org/10.3390/plants11172284

APA Style

AlHudaib, K. A., Alanazi, N. A., Ghorbel, M., El-Ganainy, S. M., & Brini, F. (2022). Isolation and Characterization of a Novel Pathogenesis-Related Protein-1 Gene (AvPR-1) with Induced Expression in Oat (Avena sativa L.) during Abiotic and Hormonal Stresses. Plants, 11(17), 2284. https://doi.org/10.3390/plants11172284

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