Overexpression of Cassava MeAnn2 Enhances the Salt and IAA Tolerance of Transgenic Arabidopsis
Abstract
:1. Introduction
2. Results
2.1. Cloning and Characterization of MeAnn2 in Cassava
2.2. Subcellular Localization of the MeAnn2 Protein
2.3. Analysis of MeAnn2 Expression in Cassava
2.4. Transformation of MeAnn2 in Arabidopsis
2.5. Effect of Different Ca2+ Concentrations on Arabidopsis Seedlings
2.6. Effects of NaCl Stress on Growth in MeAnn2-Overexpressing Arabidopsis
2.7. The Effects of IAA on MeAnn2 Overexpressing Arabidopsis
3. Discussion
4. Materials and Methods
4.1. Total RNA Extraction and PCR Amplification of the MeAnn2 Gene
4.2. MeAnn2 Gene Bioinformatics Analysis and Gene Expression
4.3. Subcellular Localization of MeAnn2:GFP
4.4. Genetic Transformation and Identification of MeAnn2 Overexpression Transgenic Lines
4.5. Transgenic Arabidopsis Stress Treatment
4.6. Quantitative RT-PCR Analysis of Stress Resistance-Related Genes
4.7. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Lin, X.; Li, R.; Zhou, Y.; Tang, F.; Wang, Y.; Lu, X.; Wang, S.; Yao, Y.; Liu, J.; Hu, X.; et al. Overexpression of Cassava MeAnn2 Enhances the Salt and IAA Tolerance of Transgenic Arabidopsis. Plants 2021, 10, 941. https://doi.org/10.3390/plants10050941
Lin X, Li R, Zhou Y, Tang F, Wang Y, Lu X, Wang S, Yao Y, Liu J, Hu X, et al. Overexpression of Cassava MeAnn2 Enhances the Salt and IAA Tolerance of Transgenic Arabidopsis. Plants. 2021; 10(5):941. https://doi.org/10.3390/plants10050941
Chicago/Turabian StyleLin, Xuejun, Ruimei Li, Yangjiao Zhou, Fenlian Tang, Yajie Wang, Xiaohua Lu, Shijia Wang, Yuan Yao, Jiao Liu, Xinwen Hu, and et al. 2021. "Overexpression of Cassava MeAnn2 Enhances the Salt and IAA Tolerance of Transgenic Arabidopsis" Plants 10, no. 5: 941. https://doi.org/10.3390/plants10050941