Identification and Sequence Analysis of a Novel Ilarvirus Infecting Sweet Cherry
Abstract
:1. Introduction
2. Results
2.1. Analysis of the HTS Data
2.2. Genome Structure and Encoded Proteins of the New Ilarvirus
2.3. Nucleotide/Amino Acid Comparisons and Phylogenetic Analysis
2.4. Presence of PrVI in Prunus spp.
3. Discussion
4. Materials and Methods
4.1. High throughput Sequencing and Bioinformatics Analysis
4.2. Sanger Sequencing of the Novel Ilarvirus Genome
4.3. Sequence Analysis and Phylogenetic Reconstructions
4.4. Mini-Scale Survey for the Presence of PrVI in Greek Orchards
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Conflicts of Interest
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Prunus spp. | Collection Date | Geographic County | Total Number of Samples | Number of Samples Positive to PrVI | Accession Numbers |
---|---|---|---|---|---|
P. avium | 2009 | Imathia | 12 | 1 | MW591551 |
2014 | MW591550 | ||||
Imathia | 10 | 2 | |||
MW591552 | |||||
2019 | Imathia | 31 | 1 | MW591554 | |
2020 | Imathia | 15 | 0 | - 1 | |
P. persica | 2013 | Arta | 12 | 0 | - |
2014 | Imathia | 11 | 0 | - | |
2019 | Imathia | 6 | 1 | MW591553 | |
2020 | Imathia | 29 | 0 | - | |
P. domestica | 2020 | Imathia | 12 | 0 | - |
SUM | 138 | 5 |
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Orfanidou, C.G.; Xing, F.; Zhou, J.; Li, S.; Katis, N.I.; Maliogka, V.I. Identification and Sequence Analysis of a Novel Ilarvirus Infecting Sweet Cherry. Plants 2021, 10, 514. https://doi.org/10.3390/plants10030514
Orfanidou CG, Xing F, Zhou J, Li S, Katis NI, Maliogka VI. Identification and Sequence Analysis of a Novel Ilarvirus Infecting Sweet Cherry. Plants. 2021; 10(3):514. https://doi.org/10.3390/plants10030514
Chicago/Turabian StyleOrfanidou, Chrysoula G., Fei Xing, Jun Zhou, Shifang Li, Nikolaos I. Katis, and Varvara I. Maliogka. 2021. "Identification and Sequence Analysis of a Novel Ilarvirus Infecting Sweet Cherry" Plants 10, no. 3: 514. https://doi.org/10.3390/plants10030514