Encapsulation of Cinnamic Acid on Plant-Based Proteins: Evaluation by HPLC, DSC and FTIR-ATR
Abstract
:1. Introduction
2. Results and Discussion
2.1. HPLC Analysis of Protein/Cinnamic Acid Complexes
2.2. DSC Analysis of Protein/Cinnamic Acid Complexes
2.3. FTIR-ATR Analysis of Protein/Cinnamic Acid Complexes
3. Materials and Methods
3.1. Materials
3.2. Formulation of Protein/Cinnamic Acid Complexes
3.3. Reverse-Phase High Performance Liquid Chromatography (RP-HPLC)
3.4. Analysis by Differential Scanning Calorimetry (DSC)
3.5. Recording of IR Spectra by Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR)
3.6. Statistical Analysis
4. Conclusions
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Amount of Protein Matrix | Protein Matrix | ||
---|---|---|---|
Pea | Almond | Pumpkin | |
1% | 35.82 ± 0.05 a | 30.37 ± 0.47 a | 41.15 ± 0.11 a |
2% | 34.95 ± 0.24 a | 30.49 ± 0.13 a | 34.13 ± 0.67 b |
5% | 32.99 ± 0.16 b | 23.63 ± 0.17 b | 29.05 ± 0.02 c |
10% | 30.69 ± 0.63 c | 20.67 ± 0.01 c | 25.58 ± 0.02 d |
Amount of Protein Matrix | Protein Matrix | ||
---|---|---|---|
Pea | Almond | Pumpkin | |
100% | 88.60 ± 0.47 a | 85.24 ± 0.07 d | 87.44 ± 0.27 a |
1% | 85.15 ± 0.39 c | 88.18 ± 0.08 a | 85.11 ± 0.25 c |
2% | 85.26 ± 0.44 c | 87.19 ± 0.20 b | 86.13 ± 0.47 b |
5% | 85.66 ± 0.38 c | 86.73 ± 0.12 b,c | 86.30 ± 0.34 b |
10% | 86.88 ± 0.33 b | 86.38 ± 0.36 c | 86.69 ± 0.39 b |
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Kopjar, M.; Buljeta, I.; Jelić, I.; Kelemen, V.; Šimunović, J.; Pichler, A. Encapsulation of Cinnamic Acid on Plant-Based Proteins: Evaluation by HPLC, DSC and FTIR-ATR. Plants 2021, 10, 2158. https://doi.org/10.3390/plants10102158
Kopjar M, Buljeta I, Jelić I, Kelemen V, Šimunović J, Pichler A. Encapsulation of Cinnamic Acid on Plant-Based Proteins: Evaluation by HPLC, DSC and FTIR-ATR. Plants. 2021; 10(10):2158. https://doi.org/10.3390/plants10102158
Chicago/Turabian StyleKopjar, Mirela, Ivana Buljeta, Ivana Jelić, Vanja Kelemen, Josip Šimunović, and Anita Pichler. 2021. "Encapsulation of Cinnamic Acid on Plant-Based Proteins: Evaluation by HPLC, DSC and FTIR-ATR" Plants 10, no. 10: 2158. https://doi.org/10.3390/plants10102158