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Article

Hypoxylonol F Isolated from Annulohypoxylon annulatum Improves Insulin Secretion by Regulating Pancreatic β-cell Metabolism

by
Dahae Lee
1,2,†,
Buyng Su Hwang
3,†,
Pilju Choi
4,†,
Taejung Kim
4,
Youngseok Kim
4,
Bong Geun Song
4,
Noriko Yamabe
2,
Gwi Seo Hwang
2,
Ki Sung Kang
2,* and
Jungyeob Ham
3,5,*
1
School of Pharmacy, Sungkyunkwan University, Suwon 16419, Korea
2
College of Korean Medicine, Gachon University, Seongnam 13120, Korea
3
Freshwater Bioresources Utilization Bureau, Nakdonggang National Institute of Biological Resources, Sangju 37242, Korea
4
Natural Products Research Institute, Korea Institute of Science and Technology, Gangneung 25451, Korea
5
Division of Bio-Medical Science and Technology, University of Science and Technology, Daejeon 34114, Korea
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Biomolecules 2019, 9(8), 335; https://doi.org/10.3390/biom9080335
Submission received: 22 June 2019 / Revised: 24 July 2019 / Accepted: 30 July 2019 / Published: 2 August 2019
(This article belongs to the Section Natural and Bio-derived Molecules)
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Abstract

Insulin plays a key role in glucose homeostasis and is hence used to treat hyperglycemia, the main characteristic of diabetes mellitus. Annulohypoxylon annulatum is an inedible ball-shaped wood-rotting fungus, and hypoxylon F is one of the major compounds of A. annulatum. The aim of this study is to evaluate the effects of hypoxylonol F isolated from A. annulatum on insulin secretion in INS-1 pancreatic β-cells and demonstrate the molecular mechanisms involved. Glucose-stimulated insulin secretion (GSIS) values were evaluated using a rat insulin ELISA kit. Moreover, the expression of proteins related to pancreatic β-cell metabolism and insulin secretion was evaluated using Western blotting. Hypoxylonol F isolated from A. annulatum was found to significantly enhance glucose-stimulated insulin secretion without inducing cytotoxicity. Additionally, hypoxylonol F enhanced insulin receptor substrate-2 (IRS-2) levels and activated the phosphatidylinositol 3-kinase/protein kinase B (PI3K/Akt) pathway. Interestingly, it also modulated the expression of peroxisome proliferator-activated receptor γ (PPARγ) and pancreatic and duodenal homeobox 1 (PDX-1). Our findings showed that A. annulatum and its bioactive compounds are capable of improving insulin secretion by pancreatic β-cells. This suggests that A. annulatum can be used as a therapeutic agent to treat diabetes.
Keywords: Annulohypoxylon annulatum; insulin; PI3K; Akt; PPARγ; PDX-1 Annulohypoxylon annulatum; insulin; PI3K; Akt; PPARγ; PDX-1
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MDPI and ACS Style

Lee, D.; Hwang, B.S.; Choi, P.; Kim, T.; Kim, Y.; Song, B.G.; Yamabe, N.; Hwang, G.S.; Kang, K.S.; Ham, J. Hypoxylonol F Isolated from Annulohypoxylon annulatum Improves Insulin Secretion by Regulating Pancreatic β-cell Metabolism. Biomolecules 2019, 9, 335. https://doi.org/10.3390/biom9080335

AMA Style

Lee D, Hwang BS, Choi P, Kim T, Kim Y, Song BG, Yamabe N, Hwang GS, Kang KS, Ham J. Hypoxylonol F Isolated from Annulohypoxylon annulatum Improves Insulin Secretion by Regulating Pancreatic β-cell Metabolism. Biomolecules. 2019; 9(8):335. https://doi.org/10.3390/biom9080335

Chicago/Turabian Style

Lee, Dahae, Buyng Su Hwang, Pilju Choi, Taejung Kim, Youngseok Kim, Bong Geun Song, Noriko Yamabe, Gwi Seo Hwang, Ki Sung Kang, and Jungyeob Ham. 2019. "Hypoxylonol F Isolated from Annulohypoxylon annulatum Improves Insulin Secretion by Regulating Pancreatic β-cell Metabolism" Biomolecules 9, no. 8: 335. https://doi.org/10.3390/biom9080335

APA Style

Lee, D., Hwang, B. S., Choi, P., Kim, T., Kim, Y., Song, B. G., Yamabe, N., Hwang, G. S., Kang, K. S., & Ham, J. (2019). Hypoxylonol F Isolated from Annulohypoxylon annulatum Improves Insulin Secretion by Regulating Pancreatic β-cell Metabolism. Biomolecules, 9(8), 335. https://doi.org/10.3390/biom9080335

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