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Volume 11
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10.3390/biom11060820
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Article

RT-QuIC Using C-Terminally Truncated α-Synuclein Forms Detects Differences in Seeding Propensity of Different Brain Regions from Synucleinopathies

by
Ilaria Poggiolini
1,
Daniel Erskine
2,
Nishant N. Vaikath
1,
Janarthanan Ponraj
3,
Said Mansour
3,
Christopher M. Morris
4 and
Omar M. A. El-Agnaf
1,*
1
Neurological Disorder Research Center, Qatar Biomedical Research Institute (QBRI), Hamad Bin Khalifa University (HBKU), Qatar Foundation, Doha 974, Qatar
2
Translational and Clinical Research Institute, Newcastle University, Newcastle upon Tyne NE2 4AA, UK
3
Core Labs, Qatar Environment and Energy Research Institute (QEERI), Hamad Bin Khalifa University (HBKU), Qatar Foundation, Doha 974, Qatar
4
Newcastle Brain Tissue Resource, Translational and Clinical Research Institute, Campus for Ageing and Vitality, Newcastle University, Newcastle upon Tyne NE4 5PL, UK
*
Author to whom correspondence should be addressed.
Biomolecules 2021, 11(6), 820; https://doi.org/10.3390/biom11060820
Submission received: 13 April 2021 / Revised: 22 May 2021 / Accepted: 25 May 2021 / Published: 31 May 2021
(This article belongs to the Special Issue α-Synuclein Amyloids & Parkinson’s Disease: On Growth, Spread, and Neurodegeneration)
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Abstract

Aggregated α-synuclein (αSyn) protein is a core pathological feature of Parkinson’s disease (PD) and dementia with Lewy bodies (DLB). Both PD and DLB demonstrate the presence of diverse intracellular α-synuclein (αSyn) species, including C-terminally truncated αSyn (C-αSyn), although it is unknown how C-αSyn species contribute to disease progression. Using recombinant C-αSyn and PD and DLB brain lysates as seeds in the real-time quaking-induced conversion (RT-QuIC) assay, we explored how C-αSyn may be involved in disease stratification. Comparing the seeding activity of aqueous-soluble fractions to detergent-soluble fractions, and using αSyn 1-130 as substrate for the RT-QuIC assay, the temporal cortex seeds differentiated PD and DLB from healthy controls. In contrast to the temporal cortex, where PD and DLB could not be distinguished, αSyn 1-130 seeded by the detergent-soluble fractions from the PD frontal cortex demonstrated greater seeding efficiency compared to the DLB frontal cortex. Moreover, proteinase K-resistant (PKres) fragments from the RT-QuIC end products using C-αSyn 1-130 or C-αSyn 1-115 were more obvious in the frontal cortex compared to the temporal cortex. Morphological examinations of RT-QuIC end products showed differences in the size of the fibrils between C-αSyn 1-130 and C-αSyn 1-115, in agreement with the RT-QuIC results. These data show that C-αSyn species can distinguish PD from DLB and suggest diversity in αSyn species across these synucleinopathies, which could play a role in disease progression.
Keywords: synucleinopathies; Parkinson’s disease; dementia with Lewy bodies; RT-QuIC; α-synuclein; C-terminally truncated αSyn synucleinopathies; Parkinson’s disease; dementia with Lewy bodies; RT-QuIC; α-synuclein; C-terminally truncated αSyn

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MDPI and ACS Style

Poggiolini, I.; Erskine, D.; Vaikath, N.N.; Ponraj, J.; Mansour, S.; Morris, C.M.; El-Agnaf, O.M.A. RT-QuIC Using C-Terminally Truncated α-Synuclein Forms Detects Differences in Seeding Propensity of Different Brain Regions from Synucleinopathies. Biomolecules 2021, 11, 820. https://doi.org/10.3390/biom11060820

AMA Style

Poggiolini I, Erskine D, Vaikath NN, Ponraj J, Mansour S, Morris CM, El-Agnaf OMA. RT-QuIC Using C-Terminally Truncated α-Synuclein Forms Detects Differences in Seeding Propensity of Different Brain Regions from Synucleinopathies. Biomolecules. 2021; 11(6):820. https://doi.org/10.3390/biom11060820

Chicago/Turabian Style

Poggiolini, Ilaria, Daniel Erskine, Nishant N. Vaikath, Janarthanan Ponraj, Said Mansour, Christopher M. Morris, and Omar M. A. El-Agnaf. 2021. "RT-QuIC Using C-Terminally Truncated α-Synuclein Forms Detects Differences in Seeding Propensity of Different Brain Regions from Synucleinopathies" Biomolecules 11, no. 6: 820. https://doi.org/10.3390/biom11060820

APA Style

Poggiolini, I., Erskine, D., Vaikath, N. N., Ponraj, J., Mansour, S., Morris, C. M., & El-Agnaf, O. M. A. (2021). RT-QuIC Using C-Terminally Truncated α-Synuclein Forms Detects Differences in Seeding Propensity of Different Brain Regions from Synucleinopathies. Biomolecules, 11(6), 820. https://doi.org/10.3390/biom11060820

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