Differential Sympathetic Activation of Adipose Tissues by Brain-Derived Neurotrophic Factor
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals and Experimental Design
2.2. Cannula Implantation
2.3. Unilateral Denervation
2.4. Cannula Placement Verification and icv Injection Procedure
2.5. Body Weight, Body Composition, and Food Intake
2.6. Plasma Lipolytic Products, Corticosterone, and Catecholamines
2.7. Catecholamine Extraction and Measurement
2.8. Determination of NETO
2.9. Analysis of Gene Expression Using Real-Time Quantitative PCR
2.10. Analysis of Protein Expression Using Western Blot
2.11. Statistical Analysis
3. Results
3.1. Cohort 1: Effects of BDNF on Whole-Animal Lipid Mobilization
3.1.1. Food Intake, Body Weight, and Body Composition
3.1.2. Circulating Factors
3.1.3. Expression of Lipolytic Proteins in Adipose Tissues
3.2. Cohort 2: Effects of BDNF on Adipose Lipolysis via Sympathetic Activation
3.2.1. Adipose Tissue NE Concentration
3.2.2. Adipose Tissue NETO
3.2.3. Adipose Tissue Gene Expression
3.2.4. Adipose Tissue Protein Expression
4. Discussion
4.1. Summary of Findings
4.2. Sympathetic Activity Indicated by NETO
4.3. Expression of Lipolytic Genes and Proteins
4.4. Potential Mechanisms of BDNF Action via SNS
4.5. Potential Sites of BDNF Action
4.6. Future Studies
4.7. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Parameter | Saline | BDNF |
---|---|---|
Glycerol (mg/L) | 4.9184 ± 0.6983 | 8.4047 ± 1.3931 * |
Free fatty acids (mmol/L) | 0.3381 ± 0.0197 | 0.4234 ± 0.0198 * |
Corticosterone (ng/mL) | 496.6319 ± 99.2159 | 403.2675 ± 116.7263 |
Norepinephrine (ng/mL) | 7.5759 ± 3.1380 | 9.2376 ± 2.3438 |
Epinephrine (ng/mL) | 51.5562 ± 22.5392 | 37.9994 ± 9.0009 |
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Zhu, Q.; Liu, X.; Glazier, B.J.; Krolick, K.N.; Yang, S.; He, J.; Lo, C.C.; Shi, H. Differential Sympathetic Activation of Adipose Tissues by Brain-Derived Neurotrophic Factor. Biomolecules 2019, 9, 452. https://doi.org/10.3390/biom9090452
Zhu Q, Liu X, Glazier BJ, Krolick KN, Yang S, He J, Lo CC, Shi H. Differential Sympathetic Activation of Adipose Tissues by Brain-Derived Neurotrophic Factor. Biomolecules. 2019; 9(9):452. https://doi.org/10.3390/biom9090452
Chicago/Turabian StyleZhu, Qi, Xian Liu, Bradley J. Glazier, Kristen N. Krolick, Shangyuwen Yang, Jingyan He, Chunmin C. Lo, and Haifei Shi. 2019. "Differential Sympathetic Activation of Adipose Tissues by Brain-Derived Neurotrophic Factor" Biomolecules 9, no. 9: 452. https://doi.org/10.3390/biom9090452