Next Article in Journal
Anti-Angiogenic Effect of Asperchalasine A Via Attenuation of VEGF Signaling
Next Article in Special Issue
Anacardium Plants: Chemical,Nutritional Composition and Biotechnological Applications
Previous Article in Journal
Insights on the Use of α-Lipoic Acid for Therapeutic Purposes
Previous Article in Special Issue
Euphorbia-Derived Natural Products with Potential for Use in Health Maintenance
Open AccessArticle

Phytochemical Composition, Antioxidant, and Antimicrobial Attributes of Different Solvent Extracts from Myrica esculenta Buch.-Ham. ex. D. Don Leaves

Research Scholar, I.K. Gujral Punjab Technical University, Kapurthala 144603, Punjab, India
Department of Pharmacology, Kota College of Pharmacy, Kota 325003, Rajasthan, India
Central Ayurveda Research Institute for Drug Development, CCRAS, Ministry of AYUSH, Government of India, Bidhannagar, Kolkata 700091, West Bengal, India
Laboratoire de Biologie des Ligneux et des Grandes Cultures, INRAUSC1328, Universitéd’Orléans, 45100 Orléans, France
Department of Pharmaceutical Chemistry and Analysis, Kota College of Pharmacy, Kota 325003, Rajasthan, India
Faculty of Medicine, University of Porto, 4200-319 Porto, Portugal
Institute for Research and Innovation in Health (i3S), University of Porto, 4200-135 Porto, Portugal
Department of Pharmacy, University of Kota, Kota 325003, Rajasthan, India
Authors to whom correspondence should be addressed.
Biomolecules 2019, 9(8), 357;
Received: 25 June 2019 / Revised: 3 August 2019 / Accepted: 5 August 2019 / Published: 9 August 2019
Background: Plant diversity is a basic source of food and medicine for local Himalayan communities. The current study was designed to assess the effect of different solvents (methanol, ethyl acetate, and water) on the phenolic profile, and the corresponding biological activity was studied. Methods: Antioxidant activity was investigated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2″-azino-bis(3-ethylbenzothiazoline-6-sulphonic) acid (ABTS) assay, while the antimicrobial activity was evaluated by disk diffusion method using various bacterial and fungal strains. Results: The outcomes demonstrated that methanol acted as the most effective solvent for polyphenols extraction, as strengthened by the liquid chromatography and mass spectroscopy (LC-MS) and fourier transform infrared spectroscopy (FTIR) analysis. M. esculenta methanol extract showed the highest DPPH and ABTS radical scavenger antioxidant activity with IC50 values of 39.29 μg/mL and 52.83 μg/mL, respectively, while the ethyl acetate and aqueous extracts revealed minimum antioxidant potential. Methanol extract also revealed higher phenolic content, 88.94 ± 0.24 mg of equivalent gallic acid (GAE)/g), measured by the Folin–Ciocalteu method, while the minimum content was recorded for aqueous extract (62.38 ± 0.14 GAE/g). The highest flavonoid content was observed for methanol extract, 67.44 ± 0.14 mg quercetin equivalent (QE)/g) measured by an aluminum chloride colorimetric method, while the lowest content was recorded for aqueous extract (35.77 ± 0.14 QE/g). Antimicrobial activity findings also reveal that the methanol extract led to a higher inhibition zone against bacterial and fungal strains. FTIR analysis reveals the presence of various functional groups, viz. alkenes, amines, carboxylic acids, amides, esters, alcohols, phenols, ketones, carboxylic acids, and aromatic compounds. This FTIR analysis could serve as a basis for the authentication of M. esculenta extracts for future industrial applications. Compounds identified by LC-MS analysis were gallic acid, myricanol, myricanone, epigallocatechin 3-O-gallate, β-sitosterol, quercetin, p-coumaric acid, palmitic acid, n-pentadecanol, n-octadecanol, stigmasterol, oleanolic acid, n-hexadecanol, cis-β-caryophyllene, lupeol, and myresculoside. Conclusion: This study suggests that the methanolic extract from M. esculenta leaves has strong antioxidant potential and could be a significant source of natural antioxidants and antimicrobials for functional foods formulation. View Full-Text
Keywords: Myrica esculenta; antioxidant; antimicrobial; phenolic composition Myrica esculenta; antioxidant; antimicrobial; phenolic composition
Show Figures

Figure 1

MDPI and ACS Style

Kabra, A.; Sharma, R.; Hano, C.; Kabra, R.; Martins, N.; Baghel, U.S. Phytochemical Composition, Antioxidant, and Antimicrobial Attributes of Different Solvent Extracts from Myrica esculenta Buch.-Ham. ex. D. Don Leaves. Biomolecules 2019, 9, 357.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Search more from Scilit
Back to TopTop