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Biomolecules 2019, 9(4), 135; https://doi.org/10.3390/biom9040135

LcNAC13 Physically Interacts with LcR1MYB1 to Coregulate Anthocyanin Biosynthesis-Related Genes during Litchi Fruit Ripening

1
Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China
2
Key Laboratory of Post-Harvest Handling of Fruits, Ministry of Agriculture, Guangzhou 510650, China
3
University of Chinese Academy of Sciences, Chinese Academy of Sciences, Beijing 100049, China
4
School of Biomedical Engineering, Wenzhou Medical University, Wenzhou 325027, China
5
Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China
*
Author to whom correspondence should be addressed.
Received: 15 February 2019 / Revised: 29 March 2019 / Accepted: 2 April 2019 / Published: 4 April 2019
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Abstract

Anthocyanin accumulation is crucial for the development of quality for most fruit. The mechanism underlying the regulation of anthocyanin biosynthesis by transcription factors in litchi fruit remains largely unknown. In this study, we isolated one NAC (NAM, ATAF1/2 and CUC2) TF gene, LcNAC13. Expression of LcNAC13 was upregulated as ripening proceeded, followed by the accumulation of anthocyanins. Electrophoretic mobility shift assay (EMSA) and transient expression assay showed that LcNAC13 could negatively regulate the expression of anthocyanin biosynthesis-related genes, including LcCHS1/2, LcCHI, LcF3H, LcF3’H, LcDFR, and LcMYB1. Furthermore, LcR1MYB1, as one R1-MYB type MYB, was identified to physically interact with LcNAC13 and reverse the effect of LcNAC13. Taken together, these results suggested that LcNAC13 and LcR1MYB1 may act together to antagonistically regulate anthocyanin biosynthesis during litchi fruit ripening, which helps to provide new insights into the regulatory networks of anthocyanin biosynthesis. View Full-Text
Keywords: litchi; anthocyanin biosynthesis; NAC; R1MYB; transcriptional regulation litchi; anthocyanin biosynthesis; NAC; R1MYB; transcriptional regulation
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Jiang, G.; Li, Z.; Song, Y.; Zhu, H.; Lin, S.; Huang, R.; Jiang, Y.; Duan, X. LcNAC13 Physically Interacts with LcR1MYB1 to Coregulate Anthocyanin Biosynthesis-Related Genes during Litchi Fruit Ripening. Biomolecules 2019, 9, 135.

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