Participation of the miR-22-HDAC4-DLCO Axis in Patients with COPD by Tobacco and Biomass
Abstract
:1. Introduction
2. Material and Methods
2.1. Study Population
2.2. Pulmonary Function Testing
2.3. Blood Samples
2.4. Isolation of Serum microRNA
2.5. Differential Expression of miRNA-22 in Serum by PCR Arrays
2.6. Validation of Samples by TaqMan RT-qPCR
2.7. Protein Quantification
2.8. Statistical Analysis
3. Results
3.1. Characteristics of Women in the Study
3.2. miRNA-22 was Upregulated in COPD by Biomass Related to COPD by Smoking
3.3. Serum HDAC4 is Higher in COPD by Biomass that COPD by Smoking
3.4. HDAC4 Positively Correlated with DLCOsb %P in COPD
4. Discussion
5. Conclusions
6. Patents
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Variable | COPD-TS | COPD-BS |
Characteristics | ||
Age (years) | 66.57 ± 6.37 | 73.27 ± 8.69* |
Height (cm) | 155.31 ± 7.57 | 146.52 ± 5.69* |
Weight (Kg) | 59.52 ± 12.13 | 56.25 ± 10.82 |
BMI (Kg/m2) | 24.79 ± 5.78 | 26.33 ± 5.04 |
Exposure Characteristics | ||
Pack-years of smoking | 36.62 ± 23.1 | 0 |
Hour-years of biomass smoke exposure | 0 | 366.88 ± 219.3 |
Physiological characteristics | ||
FEV1%P | 39.85 ± 5.36 | 39.92 ± 6.57 |
FEV1/FVC ratio | 45.63 ± 11.41 | 45.23 ± 10.08 |
DLCOsb %P | 50.96 ± 11.88 | 78.45 ± 20.47* |
6MWT (m) | 286.47 ± 174.23 | 318.91 ± 102.99 |
GOLD grades | Case numbers (%) | |
III | 16 (64) | 20 (80) |
IV | 9 (36) | 5 (20) |
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Velasco-Torres, Y.; Ruiz, V.; Montaño, M.; Pérez-Padilla, R.; Falfán-Valencia, R.; Pérez-Ramos, J.; Pérez-Bautista, O.; Ramos, C. Participation of the miR-22-HDAC4-DLCO Axis in Patients with COPD by Tobacco and Biomass. Biomolecules 2019, 9, 837. https://doi.org/10.3390/biom9120837
Velasco-Torres Y, Ruiz V, Montaño M, Pérez-Padilla R, Falfán-Valencia R, Pérez-Ramos J, Pérez-Bautista O, Ramos C. Participation of the miR-22-HDAC4-DLCO Axis in Patients with COPD by Tobacco and Biomass. Biomolecules. 2019; 9(12):837. https://doi.org/10.3390/biom9120837
Chicago/Turabian StyleVelasco-Torres, Yadira, Víctor Ruiz, Martha Montaño, Rogelio Pérez-Padilla, Ramcés Falfán-Valencia, Julia Pérez-Ramos, Oliver Pérez-Bautista, and Carlos Ramos. 2019. "Participation of the miR-22-HDAC4-DLCO Axis in Patients with COPD by Tobacco and Biomass" Biomolecules 9, no. 12: 837. https://doi.org/10.3390/biom9120837
APA StyleVelasco-Torres, Y., Ruiz, V., Montaño, M., Pérez-Padilla, R., Falfán-Valencia, R., Pérez-Ramos, J., Pérez-Bautista, O., & Ramos, C. (2019). Participation of the miR-22-HDAC4-DLCO Axis in Patients with COPD by Tobacco and Biomass. Biomolecules, 9(12), 837. https://doi.org/10.3390/biom9120837