Honokiol Enhances TRAIL-Mediated Apoptosis through STAMBPL1-Induced Survivin and c-FLIP Degradation
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Culture and Transfection
2.2. Reagents, Antibodies, siRNAs, and Plasmids
2.3. FACS Analysis
2.4. Western Blotting
2.5. DNA Fragmentation and DEVDase Activity Assay for Detection of Apoptosis
2.6. Reverse Transcription Polymerase Chain Reaction (RT-PCR) and Quantitative PCR (qPCR)
2.7. Detection of DR5 Expression on Cell Surface
2.8. Deubiquitination Assay
2.9. Immunoprecipitation
2.10. Statistical Analysis
3. Results
3.1. Honokiol Sensitizes Cancer Cells to TRAIL-Mediated Apoptosis, but Not Normal Cells
3.2. Upregulation of DR5 by Honokiol Is Not Involved in Enhancement of TRAIL Sensitivity
3.3. Downregulation of Survivin and c-FLIP Is Associated with Honokiol Plus TRAIL-Induced Apoptosis
3.4. Honokiol Induces Survivin and c-FLIP Degradation through Activation of Ubiquitin-Proteasome System
3.5. STAMBPL1 Can Regulate Survivin and c-FLIP Stability
3.6. STAMBPL1 Interacts and Induces Deubiquitination of Survivin and c-FLIP
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Woo, S.M.; Seo, S.U.; Kubatka, P.; Min, K.-j.; Kwon, T.K. Honokiol Enhances TRAIL-Mediated Apoptosis through STAMBPL1-Induced Survivin and c-FLIP Degradation. Biomolecules 2019, 9, 838. https://doi.org/10.3390/biom9120838
Woo SM, Seo SU, Kubatka P, Min K-j, Kwon TK. Honokiol Enhances TRAIL-Mediated Apoptosis through STAMBPL1-Induced Survivin and c-FLIP Degradation. Biomolecules. 2019; 9(12):838. https://doi.org/10.3390/biom9120838
Chicago/Turabian StyleWoo, Seon Min, Seung Un Seo, Peter Kubatka, Kyoung-jin Min, and Taeg Kyu Kwon. 2019. "Honokiol Enhances TRAIL-Mediated Apoptosis through STAMBPL1-Induced Survivin and c-FLIP Degradation" Biomolecules 9, no. 12: 838. https://doi.org/10.3390/biom9120838