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Open AccessArticle

Phenolic Composition Influences the Health-Promoting Potential of Bee-Pollen

1
University of Belgrade—Faculty of Chemistry P.O. Box 51, 11158 Belgrade, Serbia
2
Department of Food Chemistry, National Institute of Chemistry, Hajdrihova 19, SI-1000 Ljubljana, Slovenia
3
Institute for Biological Research “Siniša Stanković”—National Institute of Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia
4
BioSense Institute—Research Institute for Information Technologies in Biosystems, University of Novi Sad, 21000 Novi Sad, Serbia
*
Authors to whom correspondence should be addressed.
Biomolecules 2019, 9(12), 783; https://doi.org/10.3390/biom9120783
Received: 29 October 2019 / Revised: 15 November 2019 / Accepted: 17 November 2019 / Published: 26 November 2019
(This article belongs to the Special Issue Pollen as Food and Feed)
Information on compositional, nutritional and functional properties of bee-pollen, as a health-promoting food, is essential for defining its quality. Concerning the nutritional importance of phenolic compounds, the aim of this study was to determine the phenolic profile and antioxidant activity of twenty-four bee-pollen samples collected from different regions of Serbia. High-performance thin-layer chromatographic (HPTLC) fingerprinting was used for profiling of bee-pollen samples according to the botanical type. HPTLC hyphenated with image analysis and a pattern recognition technique confirmed the grouping of samples caused by the specific phenolic composition of pollens of different botanical origin. Flavonoid glycosides in bee-pollen samples were identified by applying ultra-high-performance liquid chromatography (UHPLC) coupled with linear ion trap-Orbitrap mass spectrometry (LTQ Orbitrap MS). Eight out of twenty-seven flavonol glycosides were identified in bee-pollen samples for the first time. All analyzed bee-pollen samples showed a high number of phenolic compounds which may have therapeutic potential. View Full-Text
Keywords: bee-pollen; HPTLC; UHPLC-MS/MS; phenolic profile; antioxidant activity bee-pollen; HPTLC; UHPLC-MS/MS; phenolic profile; antioxidant activity
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Mosić, M.; Trifković, J.; Vovk, I.; Gašić, U.; Tešić, Ž.; Šikoparija, B.; Milojković-Opsenica, D. Phenolic Composition Influences the Health-Promoting Potential of Bee-Pollen. Biomolecules 2019, 9, 783.

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