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Open AccessArticle

Combined Use of Deep Eutectic Solvents, Macroporous Resins, and Preparative Liquid Chromatography for the Isolation and Purification of Flavonoids and 20-Hydroxyecdysone from Chenopodium quinoa Willd.

by Jia Zeng 1,2,†, Xianchao Shang 1,2,†, Peng Zhang 1, Hongwei Wang 3,*, Yanlong Gu 4,* and Jia-Neng Tan 1,*
1
Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, Shandong 266101, China
2
Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100081, China
3
State Key Laboratory Cultivation Base, Shandong Provincial Key Laboratory of Ophthalmology, Shandong Eye Institute, Shandong First Medical University & Shandong Academy of Medical Sciences, Qingdao 266071, China
4
Key Laboratory of Energy Conversion and Storage, Ministry of Education, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Biomolecules 2019, 9(12), 776; https://doi.org/10.3390/biom9120776
Received: 16 September 2019 / Revised: 8 November 2019 / Accepted: 21 November 2019 / Published: 25 November 2019
(This article belongs to the Special Issue Phytochemical Omics in Medicinal Plants)
: Deep eutectic solvents (DESs) were used in combination with macroporous resins to isolate and purify flavonoids and 20-hydroxyecdysone from Chenopodium quinoa Willd by preparative high-performance liquid chromatography (HPLC). The extraction performances of six DESs and the adsorption/desorption performances of five resins (AB-8, D101, HPD 400, HPD 600, and NKA-9) were investigated using the total flavonoid and 20-hydroxyecdysone extraction yields as the evaluation criteria, and the best-performing DES (choline chloride/urea, DES-6) and macroporous resin (D101) were further employed for phytochemical extraction and DES removal, respectively. The purified extract was subjected to preparative HPLC, and the five collected fractions were purified in a successive round of preparative HPLC to isolate three flavonoids and 20-hydroxyecdysone, which were identified by spectroscopic techniques. The use of a DES in this study significantly facilitated the preparative-scale isolation and purification of polar phytochemicals from complex plant systems.
Keywords: DESs; preparative-scale purification; flavonoids; 20-hydroxyecdysone; Chenopodium quinoa DESs; preparative-scale purification; flavonoids; 20-hydroxyecdysone; Chenopodium quinoa
MDPI and ACS Style

Zeng, J.; Shang, X.; Zhang, P.; Wang, H.; Gu, Y.; Tan, J.-N. Combined Use of Deep Eutectic Solvents, Macroporous Resins, and Preparative Liquid Chromatography for the Isolation and Purification of Flavonoids and 20-Hydroxyecdysone from Chenopodium quinoa Willd.. Biomolecules 2019, 9, 776.

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