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The Potential Involvement of an ATP-Dependent Potassium Channel-Opening Mechanism in the Smooth Muscle Relaxant Properties of Tamarix dioica Roxb.

1
Faculty of Pharmacy, Bahauddin Zakariya University, 60800 Multan, Pakistan
2
Department of Engineering and Management of Food and Tourism, Faculty of Tourism, Transilvania University, Brasov 500036, Romania
3
Department of Fundamental, Prophylactical and Clinical Disciplines, Faculty of Medicine, Transilvania University, Brasov 500019, Romania
4
Department of Surgical and Medical Specialities, Faculty of Medicine, Transilvania University, Brasov 500019, Romania
*
Author to whom correspondence should be addressed.
Biomolecules 2019, 9(11), 722; https://doi.org/10.3390/biom9110722
Received: 17 October 2019 / Revised: 3 November 2019 / Accepted: 6 November 2019 / Published: 10 November 2019
(This article belongs to the Section Natural and Bio-inspired Molecules)
Background: Tamarix dioica is traditionally used to manage various disorders related to smooth muscle in the gastrointestinal, respiratory, and cardiovascular systems. This study was planned to establish a pharmacological basis for the uses of Tamarix dioica in certain medical conditions related to the digestive, respiratory, and cardiovascular systems, and to explore the underlying mechanisms. Methods: A phytochemical study was performed by preliminary methods, followed by HPLC-DAD and spectrometric methods. In vivo evaluation of a crude hydromethanolic extract of T. dioica (TdCr) was done with a castor-oil-provoked diarrheal model in rats to determine its antidiarrheal effect. Ex vivo experiments were done by using isolated tissues to determine the effects on smooth and cardiac muscles and explore the possible mechanisms. Results: TdCr tested positive for flavonoids, saponins, phenols, and tannins as methanolic solvable constituents in a preliminary study. The maximum quantity of gallic acid equivalent (GAE), phenolic, and quercetin equivalent (QE) flavonoid content found was 146 ± 0.001 μg GAE/mg extract and 36.17 ± 2.35 μg QE/mg extract. Quantification based on HPLC-DAD (reverse phase) exposed the presence of rutin at the highest concentration, followed by catechin, gallic acid, myricetin, kaempferol, and apigenin in TdCr. In vivo experiments showed the significant antidiarrheal effect of TdCr (100, 200, and 400 mg/kg) in the diarrheal (castor-oil-provoked) model. Ex vivo experiments revealed spasmolytic, bronchodilatory, and vasorelaxant activities as well as partial cardiac depressant activity, which may be potentiated by a potassium channel opener mechanism, similar to that of cromakalim. The potassium channel (KATP channel)-opening activity was further confirmed by repeating the experiments in glibenclamide-pretreated tissues. Conclusions: In vivo and ex vivo studies of T. dioica provided evidence of the antidiarrheal, spasmolytic, bronchodilator, vasorelaxant, and partial cardiodepressant properties facilitated through the opening of the KATP channel.
Keywords: Tamarix dioica; HPLC-DAD; antidiarrheal; spasmolytic; bronchodilator; vasodilator; KATP channel opener Tamarix dioica; HPLC-DAD; antidiarrheal; spasmolytic; bronchodilator; vasodilator; KATP channel opener
MDPI and ACS Style

Imtiaz, S.M.; Aleem, A.; Saqib, F.; Ormenisan, A.N.; Neculau, A.E.; Anastasiu, C.V. The Potential Involvement of an ATP-Dependent Potassium Channel-Opening Mechanism in the Smooth Muscle Relaxant Properties of Tamarix dioica Roxb.. Biomolecules 2019, 9, 722.

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