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Open AccessArticle

Unravelling the Skin Secretion Peptides of the Gliding Leaf Frog, Agalychnis spurrelli (Hylidae)

1
Natural Drug Discovery Group, School of Pharmacy, Queen’s University, Belfast 97 Lisburn Road, Belfast BT9 7BL, Northern Ireland, UK
2
Biomolecules Discovery Group, Laboratory of Molecular Biology and Biochemistry, Universidad Regional Amazónica Ikiam, km 7 ½ vía Muyuna, Tena 150150, Ecuador
3
Laboratorio de Investigación en Citogenética y Biomoléculas de Anfibios (LICBA), Centro de Investigación para la Salud en América Latina (CISeAL), Pontificia Universidad Católica del Ecuador, Av 12 de Octubre 1076 y Roca, Quito 170150, Ecuador
*
Author to whom correspondence should be addressed.
Academic Editor: Hamilton Cabrals
Biomolecules 2019, 9(11), 667; https://doi.org/10.3390/biom9110667
Received: 18 September 2019 / Revised: 14 October 2019 / Accepted: 16 October 2019 / Published: 30 October 2019
(This article belongs to the Special Issue Peptides: Molecular and Biotechnological Aspects)
Frog skin secretions contain medically-valuable molecules, which are useful for the discovery of new biopharmaceuticals. The peptide profile of the skin secretion of Agalychnis spurrelli has not been investigated; therefore, the structural and biological characterization of its compounds signify an inestimable opportunity to acquire new biologically-active chemical scaffolds. In this work, skin secretion from this amphibian was analysed by molecular cloning and tandem mass spectrometry. Although the extent of this work was not exhaustive, eleven skin secretion peptides belonging to five peptide families were identified. Among these, we report the occurrence of two phyllokinins, and one medusin-SP which were previously reported in other related species. In addition, eight novel peptides were identified, including four dermaseptins, DRS-SP2 to DRS-SP5, one phylloseptin-SP1, and three orphan peptides. Phylloseptin-SP1 and dermaseptins-SP2 were identified in HPLC fractions based on their molecular masses determined by MALDI-TOF MS. Among the antimicrobial peptides, dermaseptin-SP2 was the most potent, inhibiting Escherichia coli, Staphylococcus aureus, and ORSA with a minimum inhibitory concentration (MIC) of 2.68 μM, and Candida albicans with an MIC of 10.71 μM, without haemolytic effects. The peptides described in this study represent but a superficial glance at the considerable structural diversity of bioactive peptides produced in the skin secretion of A. spurrelli. View Full-Text
Keywords: dermaseptins; phylloseptins; frog skin secretion; peptidomics; tandem mass spectrometry; Agalychnis spurrelli dermaseptins; phylloseptins; frog skin secretion; peptidomics; tandem mass spectrometry; Agalychnis spurrelli
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Proaño-Bolaños, C.; Blasco-Zúñiga, A.; Almeida, J.R.; Wang, L.; Llumiquinga, M.A.; Rivera, M.; Zhou, M.; Chen, T.; Shaw, C. Unravelling the Skin Secretion Peptides of the Gliding Leaf Frog, Agalychnis spurrelli (Hylidae). Biomolecules 2019, 9, 667.

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