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Biomolecules 2018, 8(3), 68; https://doi.org/10.3390/biom8030068

Cytotoxicity of Different Concentrations of Three Root Canal Sealers on Human Mesenchymal Stem Cells

1
Department of Restorative Dental Science, College of Dentistry, King Saud University, Riyadh 11611, Saudi Arabia
2
College of Dentistry, King Saud University, P.O. Box 85676, Riyadh 11611, Saudi Arabia
3
Stem Cell Unit, Department of Anatomy, College of Medicine, King Saud University, Riyadh 11461, Saudi Arabia
4
Department of Periodontics, Saveetha Dental College and Hospitals, Saveetha University, Poonamallee High Road, Chennai 600077, India
*
Author to whom correspondence should be addressed.
Received: 12 July 2018 / Revised: 27 July 2018 / Accepted: 30 July 2018 / Published: 1 August 2018
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Abstract

This study assessed the dose-dependent effect on the cytotoxicity of BioRoot RCS (BR) and Endosequence BC (BC) sealers in human bone marrow mesenchymal stem cells (hMSCs) compared to those of the AH Plus sealer. Cells were exposed to different dilutions of extracts from freshly prepared sealers (1:2, 1:8, 1:32). Unexposed cells acted as the negative control. Cytotoxicity was evaluated by an alamar blue assay. Cell morphology was analyzed by using scanning electron microscopy after exposure to the different sealers’ extracts. Statistical analysis was performed using a one-way analysis of variance and the Bonferroni post hoc test (p < 0.05). The cytotoxicities of BC and BR were less than that of AH Plus. In the presence of 1:2 BR, the cell proliferation was significantly lower than the control. At 1:8 and 1:32 concentrations, both the tricalcium silicate sealers led to similar cellular proliferation. Cells in BC and BR sealers’ extracts spread better than those in AH Plus extract. View Full-Text
Keywords: AH Plus; BioRoot RCS; cytotoxicity; Endosequence BC; mesenchymal stem cells AH Plus; BioRoot RCS; cytotoxicity; Endosequence BC; mesenchymal stem cells
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Alsubait, S.A.; Al Ajlan, R.; Mitwalli, H.; Aburaisi, N.; Mahmood, A.; Muthurangan, M.; Almadhri, R.; Alfayez, M.; Anil, S. Cytotoxicity of Different Concentrations of Three Root Canal Sealers on Human Mesenchymal Stem Cells. Biomolecules 2018, 8, 68.

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