Next Article in Journal
Identification of a Novel Antibacterial Function of Mammalian Calreticulin
Previous Article in Journal
Endogenous Ribonucleases: Therapeutic Targeting of the Transcriptome Through Oligonucleotide-Triggered RNA Inactivation
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Biomolecules
Volume 15
Issue 7
10.3390/biom15070964
biomolecules-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Lipid Profile Characterization of Human Micro-Fragmented Adipose Tissue via Untargeted Lipidomics

by
Camillo Morano
1,*,†,
Michele Dei Cas
1,2,†,
Giulio Alessandri
2,3,
Valentina Coccè
2,
Francesca Paino
2,*,
Monica Bignotto
4,
Luisa Doneda
2,
Carlo Tremolada
3,
Augusto Pessina
2 and
Rita Paroni
1,2
1
Clinical Biochemistry and Mass Spectrometry Laboratory, Department of Health Sciences, University of Milan, 20122 Milan, Italy
2
CRC StaMeTec, Department of Biomedical, Surgical and Dental Sciences, University of Milan, 20133 Milan, Italy
3
Image Regenerative Clinic, 20122 Milan, Italy
4
Liver and Gastroenterology Unit, Department of Health Sciences, University of Milan, 20122 Milan, Italy
*
Authors to whom correspondence should be addressed.
†
The first two authors contributed equally to this work.
Biomolecules 2025, 15(7), 964; https://doi.org/10.3390/biom15070964
Submission received: 18 April 2025 / Revised: 27 June 2025 / Accepted: 2 July 2025 / Published: 4 July 2025
(This article belongs to the Section Lipids)
Browse Figures
Versions Notes

Abstract

Mesenchymal stem cells (MSCs) exhibit low immunogenicity, multipotency, and are abundantly present in adipose tissue, making this tissue an easily accessible resource for regenerative medicine. Different commercial procedures have been developed to micro-fragment the adipose tissue aspirate from patients before its reinjection. We explored a commercial device which mechanically micro-fragments human lipoaspirate (LA) resulting in a homogeneous micro-fragmentation of fat tissue (MFAT). This device has been successfully employed in several clinical applications involving autologous adipose tissue transplantation. Here, we compare the untargeted/targeted lipidomic profile of LA and MFAT looking for differences in terms of qualitative modifications occurring during the handling of the original LA material. In MFAT, different lipid subclasses such as diacylglycerols, triacylglycerols, phospholipids, and sphingolipids are more represented than in LA. In addition, via targeted fatty acids analysis, we found a lower abundance of monounsaturated fatty acids in MFAT. The biological implications of these findings must be better investigated to contribute to a better understanding of the clinical efficacy of MFAT and for its potential use as a scaffold for drug delivery applications.
Keywords: micro-fragmented adipose tissue; fat tissue; lipidomics; drug delivery; mass spectrometry micro-fragmented adipose tissue; fat tissue; lipidomics; drug delivery; mass spectrometry

Share and Cite

MDPI and ACS Style

Morano, C.; Dei Cas, M.; Alessandri, G.; Coccè, V.; Paino, F.; Bignotto, M.; Doneda, L.; Tremolada, C.; Pessina, A.; Paroni, R. Lipid Profile Characterization of Human Micro-Fragmented Adipose Tissue via Untargeted Lipidomics. Biomolecules 2025, 15, 964. https://doi.org/10.3390/biom15070964

AMA Style

Morano C, Dei Cas M, Alessandri G, Coccè V, Paino F, Bignotto M, Doneda L, Tremolada C, Pessina A, Paroni R. Lipid Profile Characterization of Human Micro-Fragmented Adipose Tissue via Untargeted Lipidomics. Biomolecules. 2025; 15(7):964. https://doi.org/10.3390/biom15070964

Chicago/Turabian Style

Morano, Camillo, Michele Dei Cas, Giulio Alessandri, Valentina Coccè, Francesca Paino, Monica Bignotto, Luisa Doneda, Carlo Tremolada, Augusto Pessina, and Rita Paroni. 2025. "Lipid Profile Characterization of Human Micro-Fragmented Adipose Tissue via Untargeted Lipidomics" Biomolecules 15, no. 7: 964. https://doi.org/10.3390/biom15070964

APA Style

Morano, C., Dei Cas, M., Alessandri, G., Coccè, V., Paino, F., Bignotto, M., Doneda, L., Tremolada, C., Pessina, A., & Paroni, R. (2025). Lipid Profile Characterization of Human Micro-Fragmented Adipose Tissue via Untargeted Lipidomics. Biomolecules, 15(7), 964. https://doi.org/10.3390/biom15070964

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop