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Biomolecules
Volume 14
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10.3390/biom14010058
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Article

Aberrant Mannosylated and Highly Fucosylated Glycoepitopes of Prostatic Acid Phosphatase as Potential Ligands for Dendritic-Cell Specific ICAM-Grabbing Nonintegrin (DC-SIGN) in Human Seminal Plasma—A Step towards Explaining Idiopathic Infertility

by
Anna Kałuża
1,*,
Katarzyna Trzęsicka
2,
Damian Drzyzga
2 and
Mirosława Ferens-Sieczkowska
1
1
Department of Biochemistry and Immunochemistry, Division of Chemistry and Immunochemistry, Wroclaw Medical University, M. Skłodowskiej-Curie 48/50, 50-369 Wroclaw, Poland
2
INVICTA, Research and Development Center, Polna 64, 81-740 Sopot, Poland
*
Author to whom correspondence should be addressed.
Biomolecules 2024, 14(1), 58; https://doi.org/10.3390/biom14010058
Submission received: 23 November 2023 / Revised: 28 December 2023 / Accepted: 30 December 2023 / Published: 31 December 2023
(This article belongs to the Special Issue Protein Glycosylation and Human Diseases)
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Abstract

Semen prostatic acid phosphatase (PAP) has been proposed as an endogenous ligand for dendritic cell-specific ICAM-3-grabbing nonintegrin (DC-SIGN), which plays a critical immuno-modulating role in maintaining homeostasis in the female reproductive tracts. In the current study, we assumed that semen PAP bears a set of fucosylated and mannosylated glycans, which may mediate the efficient binding of PAP to DC-SIGN. To investigate this hypothesis, we developed ELISA assays using Galanthus nivalis and Lotus tetragonolobus lectins capable of binding mannose-containing glycans or LewisX and LewisY motifs, respectively. In our assay with Galanthus nivalis, we detected that the relative reactivity of PAP mannose-presenting glycans in the normozoospermic idiopathic group was significantly higher than in the asthenozoospermic, oligozoospermic and oligoasthenozoospermic groups. Simultaneously, we observed slight differences in the relative reactivities of PAP glycans with Lotus tetragonolobus lectin among groups of patients with abnormal semen parameters. Subsequently, we examined whether DC-SIGN interacts with seminal plasma PAP glycans, and we detected a significantly higher relative reactivity in the normozoospermic group compared to the oligozoospermic group. Finally, we concluded that the significantly aberrant abundance of mannosylated functional groups of PAP among patients with semen disorders can suggest that PAP may thereby be engaged in modulating the immune response and promoting a tolerogenic response to male antigens in the female reproductive system.
Keywords: prostatic acid phosphatase; DC-SIGN; dendritic-cell specific ICAM-grabbing nonintegrin; seminal plasma; idiopathic infertility prostatic acid phosphatase; DC-SIGN; dendritic-cell specific ICAM-grabbing nonintegrin; seminal plasma; idiopathic infertility
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MDPI and ACS Style

Kałuża, A.; Trzęsicka, K.; Drzyzga, D.; Ferens-Sieczkowska, M. Aberrant Mannosylated and Highly Fucosylated Glycoepitopes of Prostatic Acid Phosphatase as Potential Ligands for Dendritic-Cell Specific ICAM-Grabbing Nonintegrin (DC-SIGN) in Human Seminal Plasma—A Step towards Explaining Idiopathic Infertility. Biomolecules 2024, 14, 58. https://doi.org/10.3390/biom14010058

AMA Style

Kałuża A, Trzęsicka K, Drzyzga D, Ferens-Sieczkowska M. Aberrant Mannosylated and Highly Fucosylated Glycoepitopes of Prostatic Acid Phosphatase as Potential Ligands for Dendritic-Cell Specific ICAM-Grabbing Nonintegrin (DC-SIGN) in Human Seminal Plasma—A Step towards Explaining Idiopathic Infertility. Biomolecules. 2024; 14(1):58. https://doi.org/10.3390/biom14010058

Chicago/Turabian Style

Kałuża, Anna, Katarzyna Trzęsicka, Damian Drzyzga, and Mirosława Ferens-Sieczkowska. 2024. "Aberrant Mannosylated and Highly Fucosylated Glycoepitopes of Prostatic Acid Phosphatase as Potential Ligands for Dendritic-Cell Specific ICAM-Grabbing Nonintegrin (DC-SIGN) in Human Seminal Plasma—A Step towards Explaining Idiopathic Infertility" Biomolecules 14, no. 1: 58. https://doi.org/10.3390/biom14010058

APA Style

Kałuża, A., Trzęsicka, K., Drzyzga, D., & Ferens-Sieczkowska, M. (2024). Aberrant Mannosylated and Highly Fucosylated Glycoepitopes of Prostatic Acid Phosphatase as Potential Ligands for Dendritic-Cell Specific ICAM-Grabbing Nonintegrin (DC-SIGN) in Human Seminal Plasma—A Step towards Explaining Idiopathic Infertility. Biomolecules, 14(1), 58. https://doi.org/10.3390/biom14010058

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